Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms
Actinobacillus pleuropneumoniae, the etiological agent of porcine pleuropneumonia, represents one of the most important health problems in the swine industry worldwide and it is included in the porcine respiratory disease complex. One of the bacterial survival strategies is biofilm formation, which...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2018.00184/full |
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| author | Flor Y. Ramírez-Castillo Abraham Loera-Muro Nicy D. Vargas-Padilla Adriana C. Moreno-Flores Francisco J. Avelar-González Josée Harel Mario Jacques Ricardo Oropeza Carolina C. Barajas-García Alma L. Guerrero-Barrera |
| author_facet | Flor Y. Ramírez-Castillo Abraham Loera-Muro Nicy D. Vargas-Padilla Adriana C. Moreno-Flores Francisco J. Avelar-González Josée Harel Mario Jacques Ricardo Oropeza Carolina C. Barajas-García Alma L. Guerrero-Barrera |
| author_sort | Flor Y. Ramírez-Castillo |
| collection | DOAJ |
| description | Actinobacillus pleuropneumoniae, the etiological agent of porcine pleuropneumonia, represents one of the most important health problems in the swine industry worldwide and it is included in the porcine respiratory disease complex. One of the bacterial survival strategies is biofilm formation, which are bacterial communities embedded in an extracellular matrix that could be attached to a living or an inert surface. Until recently, A. pleuropneumoniae was considered to be an obligate pathogen. However, recent studies have shown that A. pleuropneumoniae is present in farm drinking water. In this study, the drinking water microbial communities of Aguascalientes (Mexico) swine farms were analyzed, where the most frequent isolated bacterium was Escherichia coli. Biofilm formation was tested in vitro; producing E. coli biofilms under optimal growth conditions; subsequently, A. pleuropneumoniae serotype 1 (strains 4074 and 719) was incorporated to these biofilms. Interaction between both bacteria was evidenced, producing an increase in biofilm formation. Extracellular matrix composition of two-species biofilms was also characterized using fluorescent markers and enzyme treatments. In conclusion, results confirm that A. pleuropneumoniae is capable of integrates into biofilms formed by environmental bacteria, indicative of a possible survival strategy in the environment and a mechanism for disease dispersion. |
| first_indexed | 2024-12-12T03:44:02Z |
| format | Article |
| id | doaj.art-5976a3b39e5c49498fc00d3802f36fc1 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-12T03:44:02Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-5976a3b39e5c49498fc00d3802f36fc12022-12-22T00:39:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692018-08-01510.3389/fvets.2018.00184378660Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine FarmsFlor Y. Ramírez-Castillo0Abraham Loera-Muro1Nicy D. Vargas-Padilla2Adriana C. Moreno-Flores3Francisco J. Avelar-González4Josée Harel5Mario Jacques6Ricardo Oropeza7Carolina C. Barajas-García8Alma L. Guerrero-Barrera9Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, MexicoCONACYT, Centro de Investigaciones Biológicas del Noreste (CIBNOR), La Paz, MexicoDepartamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, MexicoDepartamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, MexicoDepartamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, MexicoGroupe de Recherche sur la Maladies Infectieuses en Production Animale (GREMIP), Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, QC, CanadaGroupe de Recherche sur la Maladies Infectieuses en Production Animale (GREMIP), Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, QC, CanadaDepartamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, MexicoDepartamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, MexicoDepartamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, MexicoActinobacillus pleuropneumoniae, the etiological agent of porcine pleuropneumonia, represents one of the most important health problems in the swine industry worldwide and it is included in the porcine respiratory disease complex. One of the bacterial survival strategies is biofilm formation, which are bacterial communities embedded in an extracellular matrix that could be attached to a living or an inert surface. Until recently, A. pleuropneumoniae was considered to be an obligate pathogen. However, recent studies have shown that A. pleuropneumoniae is present in farm drinking water. In this study, the drinking water microbial communities of Aguascalientes (Mexico) swine farms were analyzed, where the most frequent isolated bacterium was Escherichia coli. Biofilm formation was tested in vitro; producing E. coli biofilms under optimal growth conditions; subsequently, A. pleuropneumoniae serotype 1 (strains 4074 and 719) was incorporated to these biofilms. Interaction between both bacteria was evidenced, producing an increase in biofilm formation. Extracellular matrix composition of two-species biofilms was also characterized using fluorescent markers and enzyme treatments. In conclusion, results confirm that A. pleuropneumoniae is capable of integrates into biofilms formed by environmental bacteria, indicative of a possible survival strategy in the environment and a mechanism for disease dispersion.https://www.frontiersin.org/article/10.3389/fvets.2018.00184/fullActinobacillus pleuropneumoniaeEscherichia colirespiratory pathogenpleuropneumoniabiofilmsdrinking water |
| spellingShingle | Flor Y. Ramírez-Castillo Abraham Loera-Muro Nicy D. Vargas-Padilla Adriana C. Moreno-Flores Francisco J. Avelar-González Josée Harel Mario Jacques Ricardo Oropeza Carolina C. Barajas-García Alma L. Guerrero-Barrera Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms Frontiers in Veterinary Science Actinobacillus pleuropneumoniae Escherichia coli respiratory pathogen pleuropneumonia biofilms drinking water |
| title | Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms |
| title_full | Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms |
| title_fullStr | Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms |
| title_full_unstemmed | Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms |
| title_short | Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms |
| title_sort | incorporation of actinobacillus pleuropneumoniae in preformed biofilms by escherichia coli isolated from drinking water of swine farms |
| topic | Actinobacillus pleuropneumoniae Escherichia coli respiratory pathogen pleuropneumonia biofilms drinking water |
| url | https://www.frontiersin.org/article/10.3389/fvets.2018.00184/full |
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