Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model
Abstract Background and aim Deleterious cutaneous tissue damages could result from exposure to thermal trauma, which could be ameliorated structurally and functionally through therapy via the most multipotent progenitor bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to induce burns a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-021-02365-y |
| _version_ | 1818832806055247872 |
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| author | Doaa Ramadan I. Abdel-Gawad Walaa A. Moselhy Rasha Rashad Ahmed Hessah Mohammed Al-Muzafar Kamal Adel Amin Maha Mohamed Amin El-Shaymaa El-Nahass Khaled Abbas Helmy Abdou |
| author_facet | Doaa Ramadan I. Abdel-Gawad Walaa A. Moselhy Rasha Rashad Ahmed Hessah Mohammed Al-Muzafar Kamal Adel Amin Maha Mohamed Amin El-Shaymaa El-Nahass Khaled Abbas Helmy Abdou |
| author_sort | Doaa Ramadan I. Abdel-Gawad |
| collection | DOAJ |
| description | Abstract Background and aim Deleterious cutaneous tissue damages could result from exposure to thermal trauma, which could be ameliorated structurally and functionally through therapy via the most multipotent progenitor bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to induce burns and examine the effect of BM-MSCs during a short and long period of therapy. Material and methods Ninety albino rats were divided into three groups: group I (control); group II (burn model), the animals were exposed to the preheated aluminum bar at 100°C for 15 s; and group III (the burned animals subcutaneously injected with BM-MSCs (2×106 cells/ ml)); they were clinically observed and sacrificed at different short and long time intervals, and skin samples were collected for histopathological and immunohistochemical examination and analysis of different wound healing mediators via quantitative polymerase chain reaction (qPCR). Results Subcutaneous injection of BM-MSCs resulted in the decrease of the wound contraction rate; the wound having a pinpoint appearance and regular arrangement of the epidermal layer with thin stratum corneum; decrease in the area percentages of ADAMs10 expression; significant downregulation of transforming growth factor-β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), metalloproteinase-9 (MMP-9), and microRNA-21; and marked upregulation of heat shock protein-90α (HSP-90α) especially in late stages. Conclusion BM-MSCs exhibited a powerful healing property through regulating the mediators of wound healing and restoring the normal skin structures, reducing the scar formation and the wound size. |
| first_indexed | 2024-12-19T02:08:52Z |
| format | Article |
| id | doaj.art-59790aedc54945c19c25021fad05016e |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-19T02:08:52Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-59790aedc54945c19c25021fad05016e2022-12-21T20:40:52ZengBMCStem Cell Research & Therapy1757-65122021-05-0112111610.1186/s13287-021-02365-yTherapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn modelDoaa Ramadan I. Abdel-Gawad0Walaa A. Moselhy1Rasha Rashad Ahmed2Hessah Mohammed Al-Muzafar3Kamal Adel Amin4Maha Mohamed Amin5El-Shaymaa El-Nahass6Khaled Abbas Helmy Abdou7Toxicology and Forensic Medicine Department, Faculty of Veterinary Medicine, Beni-Suef UniversityToxicology and Forensic Medicine Department, Faculty of Veterinary Medicine, Beni-Suef UniversityZoology Department, Faculty of Science, Beni-Suef UniversityDepartment of Chemistry, College of Science, Basic & Applied Scientific Research Center, Imam Abdulrahman Bin Faisal UniversityDepartment of Chemistry, College of Science, Basic & Applied Scientific Research Center, Imam Abdulrahman Bin Faisal UniversityPathology Department, Faculty of Medicine, Mansoura UniversityVeterinary Pathology Department, Faculty of Veterinary Medicine, Beni-Suef UniversityToxicology and Forensic Medicine Department, Faculty of Veterinary Medicine, Beni-Suef UniversityAbstract Background and aim Deleterious cutaneous tissue damages could result from exposure to thermal trauma, which could be ameliorated structurally and functionally through therapy via the most multipotent progenitor bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to induce burns and examine the effect of BM-MSCs during a short and long period of therapy. Material and methods Ninety albino rats were divided into three groups: group I (control); group II (burn model), the animals were exposed to the preheated aluminum bar at 100°C for 15 s; and group III (the burned animals subcutaneously injected with BM-MSCs (2×106 cells/ ml)); they were clinically observed and sacrificed at different short and long time intervals, and skin samples were collected for histopathological and immunohistochemical examination and analysis of different wound healing mediators via quantitative polymerase chain reaction (qPCR). Results Subcutaneous injection of BM-MSCs resulted in the decrease of the wound contraction rate; the wound having a pinpoint appearance and regular arrangement of the epidermal layer with thin stratum corneum; decrease in the area percentages of ADAMs10 expression; significant downregulation of transforming growth factor-β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), metalloproteinase-9 (MMP-9), and microRNA-21; and marked upregulation of heat shock protein-90α (HSP-90α) especially in late stages. Conclusion BM-MSCs exhibited a powerful healing property through regulating the mediators of wound healing and restoring the normal skin structures, reducing the scar formation and the wound size.https://doi.org/10.1186/s13287-021-02365-yBM-MSCsBurnHistopathologicalImmunohistochemicalqPCR |
| spellingShingle | Doaa Ramadan I. Abdel-Gawad Walaa A. Moselhy Rasha Rashad Ahmed Hessah Mohammed Al-Muzafar Kamal Adel Amin Maha Mohamed Amin El-Shaymaa El-Nahass Khaled Abbas Helmy Abdou Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model Stem Cell Research & Therapy BM-MSCs Burn Histopathological Immunohistochemical qPCR |
| title | Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model |
| title_full | Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model |
| title_fullStr | Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model |
| title_full_unstemmed | Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model |
| title_short | Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model |
| title_sort | therapeutic effect of mesenchymal stem cells on histopathological immunohistochemical and molecular analysis in second grade burn model |
| topic | BM-MSCs Burn Histopathological Immunohistochemical qPCR |
| url | https://doi.org/10.1186/s13287-021-02365-y |
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