Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus)
Background and Aim: Paramphistomiasis is common in tropical countries such as Indonesia and affects livestock and various endemic wild animals such as Sumatran elephants. However, the specific species of paramphistomoid worm that causes paramphistomiasis are rarely reported. The study aims at identi...
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Veterinary World
2019-08-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.12/August-2019/24.pdf |
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| author | Lintang Winantya Firdausy Rahmania Prahardani Lu'lu' Sahara Wusahaningtyas Soedarmanto Indarjulianto Muhammad Wahyu Muhammad Tauhid Nursalim Wisnu Nurcahyo |
| author_facet | Lintang Winantya Firdausy Rahmania Prahardani Lu'lu' Sahara Wusahaningtyas Soedarmanto Indarjulianto Muhammad Wahyu Muhammad Tauhid Nursalim Wisnu Nurcahyo |
| author_sort | Lintang Winantya Firdausy |
| collection | DOAJ |
| description | Background and Aim: Paramphistomiasis is common in tropical countries such as Indonesia and affects livestock and various endemic wild animals such as Sumatran elephants. However, the specific species of paramphistomoid worm that causes paramphistomiasis are rarely reported. The study aims at identifying paramphistomoid worm that infects Sumatran elephants.
Materials and Methods: Flukes were collected from the feces of five semi-captive Sumatran elephants that lived at Tegal Yoso Elephant Response Unit in Way Kambas National Park, in 2018, after treatment of oxyclozanide 1 g at the dose of approximately 5-8 mg/kg of body weight. Eight paramphistomoid worms were flattened and stained in Semichon's carmine for morphological identification, and five other worms were used for molecular identification at second internal transcribed spacer (ITS-2) of ribosomal deoxyribonucleic acid sequence.
Results: Forty-five flukes were collected from five Sumatran elephants in Lampung, Indonesia. Eight paramphistomoid worms were morphologically identified as Pfenderius heterocaeca> and five isolates did not show any variation in ITS-2. Phylogenetic analysis showed that there was a close genetic relationship between our sample and Chiorchis fabaceus that had a family similar to the samples.
Conclusion: Based on the morphological and molecular characteristics, the paramphistomoids found in Sumatran elephant on Way Kambas National Park are P. heterocaeca. |
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| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T17:19:02Z |
| publishDate | 2019-08-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-597995a536f9480f91cb08e2a044bbc52022-12-21T22:23:13ZengVeterinary WorldVeterinary World0972-89882231-09162019-08-011281341134510.14202/vetworld.2019.1341-1345Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus)Lintang Winantya Firdausy0Rahmania Prahardani1Lu'lu' Sahara Wusahaningtyas2Soedarmanto Indarjulianto3Muhammad Wahyu4Muhammad Tauhid Nursalim 5Wisnu Nurcahyo 6Veterinary Science Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Veterinary Science Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Veterinary Science Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Veterinary Society for Sumatran Wildlife Conservation, Medan, Indonesia.Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Background and Aim: Paramphistomiasis is common in tropical countries such as Indonesia and affects livestock and various endemic wild animals such as Sumatran elephants. However, the specific species of paramphistomoid worm that causes paramphistomiasis are rarely reported. The study aims at identifying paramphistomoid worm that infects Sumatran elephants. Materials and Methods: Flukes were collected from the feces of five semi-captive Sumatran elephants that lived at Tegal Yoso Elephant Response Unit in Way Kambas National Park, in 2018, after treatment of oxyclozanide 1 g at the dose of approximately 5-8 mg/kg of body weight. Eight paramphistomoid worms were flattened and stained in Semichon's carmine for morphological identification, and five other worms were used for molecular identification at second internal transcribed spacer (ITS-2) of ribosomal deoxyribonucleic acid sequence. Results: Forty-five flukes were collected from five Sumatran elephants in Lampung, Indonesia. Eight paramphistomoid worms were morphologically identified as Pfenderius heterocaeca> and five isolates did not show any variation in ITS-2. Phylogenetic analysis showed that there was a close genetic relationship between our sample and Chiorchis fabaceus that had a family similar to the samples. Conclusion: Based on the morphological and molecular characteristics, the paramphistomoids found in Sumatran elephant on Way Kambas National Park are P. heterocaeca.http://www.veterinaryworld.org/Vol.12/August-2019/24.pdfinternal transcribed spacer-2paramphistomiasisPfenderius spp.Sumatran elephant |
| spellingShingle | Lintang Winantya Firdausy Rahmania Prahardani Lu'lu' Sahara Wusahaningtyas Soedarmanto Indarjulianto Muhammad Wahyu Muhammad Tauhid Nursalim Wisnu Nurcahyo Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus) Veterinary World internal transcribed spacer-2 paramphistomiasis Pfenderius spp. Sumatran elephant |
| title | Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus) |
| title_full | Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus) |
| title_fullStr | Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus) |
| title_full_unstemmed | Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus) |
| title_short | Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus) |
| title_sort | morphological and molecular identification of pfenderius heterocaeca trematode paramphistomoidea from sumatran elephant elephas maximus sumatranus |
| topic | internal transcribed spacer-2 paramphistomiasis Pfenderius spp. Sumatran elephant |
| url | http://www.veterinaryworld.org/Vol.12/August-2019/24.pdf |
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