Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents
The intrinsic steady-state and time-resolved fluorescence of <i>Leishmania major</i> pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | Physchem |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-7167/2/2/10 |
| Summary: | The intrinsic steady-state and time-resolved fluorescence of <i>Leishmania major</i> pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals from these local fluorometric reporters contain molecular-level information on the conformational landscape of this protein and on its interaction with a nanomolar pteridinic inhibitor. |
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| ISSN: | 2673-7167 |