Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery
Glycerol organosolv (GO) pretreatment has been revealed to be potent in selectively deconstructing the lignocellulosic biomass and effectively enhancing its enzymatic hydrolysis, but the conventional solid washing and GO lignin extraction processes frequently consume large amounts of water, resultin...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2023-01-01
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| Series: | JPhys Energy |
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| Online Access: | https://doi.org/10.1088/2515-7655/acacc7 |
| _version_ | 1797844597032878080 |
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| author | Guojie Song Chihe Sun Yun Hu Chen Wang Changlei Xia Maobing Tu Ezhen Zhang Pau-Loke Show Fubao Sun |
| author_facet | Guojie Song Chihe Sun Yun Hu Chen Wang Changlei Xia Maobing Tu Ezhen Zhang Pau-Loke Show Fubao Sun |
| author_sort | Guojie Song |
| collection | DOAJ |
| description | Glycerol organosolv (GO) pretreatment has been revealed to be potent in selectively deconstructing the lignocellulosic biomass and effectively enhancing its enzymatic hydrolysis, but the conventional solid washing and GO lignin extraction processes frequently consume large amounts of water, resulting additionally in difficulty recycling the glycerol. In this study, an anhydrous two-step organosolv pretreatment process was explored, followed by the membrane ultrafiltration of glycerol lignin. The results showed that the solid washing of the residual glycerol after the atmospheric glycerol organosolv (AGO) pretreatment was necessary for the subsequent operation of high-solid enzymatic hydrolysis. Washing with ethanol was desirable as an alternative to water as only a low glycerol content of 5.2% resided in the substrate. Membrane ultrafiltration was helpful in extracting the AGO lignin from the pretreatment liquor, in which a high lignin extraction of 81.5% was made with a regenerated cellulose membrane (cut-off for 1 kDa) under selected ultrafiltration conditions. With the characterization of membrane-extracted lignin, it was observed for the first time that the AGO lignin has a well-preserved structure of G/S type. Moreover, the lignin was enriched with reactive groups, i.e. β -O-4′ linkages and aliphatic hydroxyl groups, which was very likely due to the glycerol grafting onto the lignin via α -etherification reaction. The two-step organosolv pretreatment process allowed 86% of glycerol and 92% of the ethanol recovery with ∼78% of distillation energy savings, which was applicable for extraction of organosolv lignin and recycling use of organic solvents. |
| first_indexed | 2024-04-09T17:25:53Z |
| format | Article |
| id | doaj.art-597b5412f9054477bb58b66a8888fb68 |
| institution | Directory Open Access Journal |
| issn | 2515-7655 |
| language | English |
| last_indexed | 2024-04-09T17:25:53Z |
| publishDate | 2023-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | JPhys Energy |
| spelling | doaj.art-597b5412f9054477bb58b66a8888fb682023-04-18T13:50:32ZengIOP PublishingJPhys Energy2515-76552023-01-015101401510.1088/2515-7655/acacc7Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recoveryGuojie Song0Chihe Sun1Yun Hu2Chen Wang3Changlei Xia4Maobing Tu5Ezhen Zhang6Pau-Loke Show7Fubao Sun8https://orcid.org/0000-0002-1208-469XKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122, People’s Republic of ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122, People’s Republic of ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122, People’s Republic of ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122, People’s Republic of ChinaJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Materials Science and Engineering, Nanjing Forestry University , Nanjing, Jiangsu 210037, People’s Republic of ChinaDepartment of Chemical and Environmental Engineering, University of Cincinnati , Cincinnati, OH 45221, United States of AmericaInstitute of Agro-Products Processing Science and Technology, Guangxi Academy of Agricultural Sciences , Nanning 530007, People’s Republic of ChinaDepartment of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus , Jalan Broga Semenyih, Semenyih, Selangor Darul Ehsan 43500, MalaysiaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122, People’s Republic of ChinaGlycerol organosolv (GO) pretreatment has been revealed to be potent in selectively deconstructing the lignocellulosic biomass and effectively enhancing its enzymatic hydrolysis, but the conventional solid washing and GO lignin extraction processes frequently consume large amounts of water, resulting additionally in difficulty recycling the glycerol. In this study, an anhydrous two-step organosolv pretreatment process was explored, followed by the membrane ultrafiltration of glycerol lignin. The results showed that the solid washing of the residual glycerol after the atmospheric glycerol organosolv (AGO) pretreatment was necessary for the subsequent operation of high-solid enzymatic hydrolysis. Washing with ethanol was desirable as an alternative to water as only a low glycerol content of 5.2% resided in the substrate. Membrane ultrafiltration was helpful in extracting the AGO lignin from the pretreatment liquor, in which a high lignin extraction of 81.5% was made with a regenerated cellulose membrane (cut-off for 1 kDa) under selected ultrafiltration conditions. With the characterization of membrane-extracted lignin, it was observed for the first time that the AGO lignin has a well-preserved structure of G/S type. Moreover, the lignin was enriched with reactive groups, i.e. β -O-4′ linkages and aliphatic hydroxyl groups, which was very likely due to the glycerol grafting onto the lignin via α -etherification reaction. The two-step organosolv pretreatment process allowed 86% of glycerol and 92% of the ethanol recovery with ∼78% of distillation energy savings, which was applicable for extraction of organosolv lignin and recycling use of organic solvents.https://doi.org/10.1088/2515-7655/acacc7two-step organosolv pretreatmentsugarcane bagasselignin fractionationmembrane ultrafiltrationsolvent recoverygrafting modification |
| spellingShingle | Guojie Song Chihe Sun Yun Hu Chen Wang Changlei Xia Maobing Tu Ezhen Zhang Pau-Loke Show Fubao Sun Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery JPhys Energy two-step organosolv pretreatment sugarcane bagasse lignin fractionation membrane ultrafiltration solvent recovery grafting modification |
| title | Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery |
| title_full | Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery |
| title_fullStr | Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery |
| title_full_unstemmed | Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery |
| title_short | Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery |
| title_sort | construction of anhydrous two step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane extraction and solvent recovery |
| topic | two-step organosolv pretreatment sugarcane bagasse lignin fractionation membrane ultrafiltration solvent recovery grafting modification |
| url | https://doi.org/10.1088/2515-7655/acacc7 |
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