Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile
Summary: Nitric oxide (NO) is a gasotransmitter required in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show that NO is accumulated in developi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724004194 |
| _version_ | 1827284842707943424 |
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| author | Inmaculada Sánchez-Vicente Pablo Albertos Carlos Sanz Brecht Wybouw Bert De Rybel Juan C. Begara-Morales Mounira Chaki Capilla Mata-Pérez Juan B. Barroso Oscar Lorenzo |
| author_facet | Inmaculada Sánchez-Vicente Pablo Albertos Carlos Sanz Brecht Wybouw Bert De Rybel Juan C. Begara-Morales Mounira Chaki Capilla Mata-Pérez Juan B. Barroso Oscar Lorenzo |
| author_sort | Inmaculada Sánchez-Vicente |
| collection | DOAJ |
| description | Summary: Nitric oxide (NO) is a gasotransmitter required in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show that NO is accumulated in developing embryos and regulates the fatty acid profile through the stabilization of the basic/leucine zipper transcription factor bZIP67. NO and nitro-linolenic acid target and accumulate bZIP67 to induce the downstream expression of FAD3 desaturase, which is misregulated in a non-nitrosylable version of the protein. Moreover, the post-translational modification of bZIP67 is reversible by the trans-denitrosylation activity of peroxiredoxin IIE and defines a feedback mechanism for bZIP67 redox regulation. These findings provide a molecular framework to control the seed fatty acid profile caused by NO, and evidence of the in vivo functionality of nitro-fatty acids during plant developmental signaling. |
| first_indexed | 2024-04-24T10:04:09Z |
| format | Article |
| id | doaj.art-597d71a51bd04d7bb3e3cdb2e6f5833f |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-24T10:04:09Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-597d71a51bd04d7bb3e3cdb2e6f5833f2024-04-13T04:21:13ZengElsevierCell Reports2211-12472024-04-01434114091Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profileInmaculada Sánchez-Vicente0Pablo Albertos1Carlos Sanz2Brecht Wybouw3Bert De Rybel4Juan C. Begara-Morales5Mounira Chaki6Capilla Mata-Pérez7Juan B. Barroso8Oscar Lorenzo9Department of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, SpainDepartment of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, Spain; Corresponding authorDepartment Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa-CSIC, Campus Universidad Pablo de Olavide, Ctra Utrera km 1, 41013 Sevilla, SpainGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, 9052 Ghent, BelgiumGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, 9052 Ghent, BelgiumDepartment of Experimental Biology, Facultad de Ciencias Experimentales, Campus Universitario “Las Lagunillas” s/n, Universidad de Jaén, E-23071 Jaén, SpainDepartment of Experimental Biology, Facultad de Ciencias Experimentales, Campus Universitario “Las Lagunillas” s/n, Universidad de Jaén, E-23071 Jaén, SpainDepartment of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, SpainDepartment of Experimental Biology, Facultad de Ciencias Experimentales, Campus Universitario “Las Lagunillas” s/n, Universidad de Jaén, E-23071 Jaén, SpainDepartment of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, Spain; Corresponding authorSummary: Nitric oxide (NO) is a gasotransmitter required in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show that NO is accumulated in developing embryos and regulates the fatty acid profile through the stabilization of the basic/leucine zipper transcription factor bZIP67. NO and nitro-linolenic acid target and accumulate bZIP67 to induce the downstream expression of FAD3 desaturase, which is misregulated in a non-nitrosylable version of the protein. Moreover, the post-translational modification of bZIP67 is reversible by the trans-denitrosylation activity of peroxiredoxin IIE and defines a feedback mechanism for bZIP67 redox regulation. These findings provide a molecular framework to control the seed fatty acid profile caused by NO, and evidence of the in vivo functionality of nitro-fatty acids during plant developmental signaling.http://www.sciencedirect.com/science/article/pii/S2211124724004194CP: Plants |
| spellingShingle | Inmaculada Sánchez-Vicente Pablo Albertos Carlos Sanz Brecht Wybouw Bert De Rybel Juan C. Begara-Morales Mounira Chaki Capilla Mata-Pérez Juan B. Barroso Oscar Lorenzo Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile Cell Reports CP: Plants |
| title | Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile |
| title_full | Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile |
| title_fullStr | Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile |
| title_full_unstemmed | Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile |
| title_short | Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile |
| title_sort | reversible s nitrosylation of bzip67 by peroxiredoxin iie activity and nitro fatty acids regulates the plant lipid profile |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724004194 |
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