Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit
Background: The aim of this study was to isolate, characterize, and investigate the efficacy of specific phages against multidrug resistant (MDR) Acinetobacter baumannii to use them as an appropriate and effective method to replace antibiotic treatment, or be used as an antiseptic for the hospital s...
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Isfahan University of Medical Sciences
2019-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/11342 |
| _version_ | 1797720552299823104 |
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| author | Behnam Sisakhtpour Vajihe Karbasizadeh Saeedeh Ebrahimi Nafiseh Sadat Hosseini Arezoo Mirzaee Mehdi Shabani Shabani Reyhaneh Jafari Sharareh Moghim |
| author_facet | Behnam Sisakhtpour Vajihe Karbasizadeh Saeedeh Ebrahimi Nafiseh Sadat Hosseini Arezoo Mirzaee Mehdi Shabani Shabani Reyhaneh Jafari Sharareh Moghim |
| author_sort | Behnam Sisakhtpour |
| collection | DOAJ |
| description | Background: The aim of this study was to isolate, characterize, and investigate the efficacy of specific phages against multidrug resistant (MDR) Acinetobacter baumannii to use them as an appropriate and effective method to replace antibiotic treatment, or be used as an antiseptic for the hospital surfaces.
Methods: In a cross-sectional study in a period of two years (2017-2019), 48 suspected Acinetobacter baumannii strains were isolated from patients admitted to the intensive care unit (ICU) in Alzahra hospital, Isfahan, Iran. First, the isolates were identified by phenotypic method, and confirmed with blaOXA-51 gene amplification. Then, the antibiotic sensitivity of isolates was assessed using disk diffusion method, and the isolation of phage from water sources was carried out. Finally, the sensitivity of Acinetobacter baumannii isolates, and different hosts to phage were investigated, and One-Step Growth Curve was detected.
Findings: All Acinetobacter baumannii isolates were confirmed, and the pattern of antibiotic resistance percentage was 99% to ciprofloxacin, 96% to ceftazidime, 97% to cefipime, 82% to amikacin, and 100% to imipenem. The specific phage was isolated from the sewage, 27 isolates of Acinetobacter bumannii were sensitive to phage, and other different hosts were not affected by the phages. Finally, after 30 minutes in the latent phase, phage was exploded during a period of 40 minutes.
Conclusion: According to the results of the effectiveness of phage in disturbing Acinetobacter baumanni, and considering the increasing rate of the resistance of it to most of the antibiotics and the difficulty of treating its infections, phage therapy can be used as a therapeutic approach for this infection. |
| first_indexed | 2024-03-12T09:21:10Z |
| format | Article |
| id | doaj.art-59822576d8944e59b26f93a59c6e101c |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:21:10Z |
| publishDate | 2019-08-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-59822576d8944e59b26f93a59c6e101c2023-09-02T14:27:36ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2019-08-013752856457110.22122/jims.v37i528.113423426Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care UnitBehnam Sisakhtpour0Vajihe Karbasizadeh1Saeedeh Ebrahimi2Nafiseh Sadat Hosseini3Arezoo Mirzaee4Mehdi Shabani Shabani5Reyhaneh Jafari6Sharareh Moghim7MSc Student, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranMSc Student, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranPhD Candidate, Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranPhD Candidate, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, IranAssociate Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: The aim of this study was to isolate, characterize, and investigate the efficacy of specific phages against multidrug resistant (MDR) Acinetobacter baumannii to use them as an appropriate and effective method to replace antibiotic treatment, or be used as an antiseptic for the hospital surfaces. Methods: In a cross-sectional study in a period of two years (2017-2019), 48 suspected Acinetobacter baumannii strains were isolated from patients admitted to the intensive care unit (ICU) in Alzahra hospital, Isfahan, Iran. First, the isolates were identified by phenotypic method, and confirmed with blaOXA-51 gene amplification. Then, the antibiotic sensitivity of isolates was assessed using disk diffusion method, and the isolation of phage from water sources was carried out. Finally, the sensitivity of Acinetobacter baumannii isolates, and different hosts to phage were investigated, and One-Step Growth Curve was detected. Findings: All Acinetobacter baumannii isolates were confirmed, and the pattern of antibiotic resistance percentage was 99% to ciprofloxacin, 96% to ceftazidime, 97% to cefipime, 82% to amikacin, and 100% to imipenem. The specific phage was isolated from the sewage, 27 isolates of Acinetobacter bumannii were sensitive to phage, and other different hosts were not affected by the phages. Finally, after 30 minutes in the latent phase, phage was exploded during a period of 40 minutes. Conclusion: According to the results of the effectiveness of phage in disturbing Acinetobacter baumanni, and considering the increasing rate of the resistance of it to most of the antibiotics and the difficulty of treating its infections, phage therapy can be used as a therapeutic approach for this infection.http://jims.mui.ac.ir/index.php/jims/article/view/11342Acinetobacter baumanniiMultidrug resistanceBacteriophagesPhage therapy |
| spellingShingle | Behnam Sisakhtpour Vajihe Karbasizadeh Saeedeh Ebrahimi Nafiseh Sadat Hosseini Arezoo Mirzaee Mehdi Shabani Shabani Reyhaneh Jafari Sharareh Moghim Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit مجله دانشکده پزشکی اصفهان Acinetobacter baumannii Multidrug resistance Bacteriophages Phage therapy |
| title | Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit |
| title_full | Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit |
| title_fullStr | Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit |
| title_full_unstemmed | Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit |
| title_short | Investigation of the Characteristics of Multidrug-Resistant (MDR) Acinetobacter Baumannii Lytic Phage Isolated from the Intensive Care Unit |
| title_sort | investigation of the characteristics of multidrug resistant mdr acinetobacter baumannii lytic phage isolated from the intensive care unit |
| topic | Acinetobacter baumannii Multidrug resistance Bacteriophages Phage therapy |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/11342 |
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