Screening of rapd primer for teak (Tectona grandis)
Identification of DNA polymorphisms in teak is important. It is a first step to determine the presence of genetic varia-tion in teak. The information of genetic variation is needed for teak breeding development. RAPD is one of method which can be used for identification of DNA polymorphism. This stu...
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| Format: | Article |
| Language: | English |
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Indonesian Biological Society
2016-04-01
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| Series: | Journal of Biological Researches |
| Subjects: | |
| Online Access: | http://berkalahayati.org/journal/10f760a8 |
| _version_ | 1819032520232009728 |
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| author | Imas Cintamulya |
| author_facet | Imas Cintamulya |
| author_sort | Imas Cintamulya |
| collection | DOAJ |
| description | Identification of DNA polymorphisms in teak is important. It is a first step to determine the presence of genetic varia-tion in teak. The information of genetic variation is needed for teak breeding development. RAPD is one of method which can be used for identification of DNA polymorphism. This study aim to get the RAPD primer which can detect the DNA polymorphism in teak. Benefits of this study are provide information about primer which can detect the DNA polymorphism in teak, DNA polymorphism data can be used for genetic variation analysis which needed for teak breeding development. The primers which used in this study shown the DNA polymorphism in teak. The primer are OPF6 (5'-GGGAATTCGG-3 '), OPF8 (5'-GGGATATCGC-3'), and OPF11 (5'-TTGGTACCCC-3 '). The highest DNA poly-morphism is shown in DNA which amplified with OPF-8 primer. Keywords: RAPD, Primer, Polymorphism, DNA, Tectona grandis |
| first_indexed | 2024-12-21T07:03:15Z |
| format | Article |
| id | doaj.art-5984e6e492c14602a0568f1cf1d0e8d3 |
| institution | Directory Open Access Journal |
| issn | 0852-6834 0852-6834 |
| language | English |
| last_indexed | 2024-12-21T07:03:15Z |
| publishDate | 2016-04-01 |
| publisher | Indonesian Biological Society |
| record_format | Article |
| series | Journal of Biological Researches |
| spelling | doaj.art-5984e6e492c14602a0568f1cf1d0e8d32022-12-21T19:12:10ZengIndonesian Biological SocietyJournal of Biological Researches0852-68340852-68342016-04-01211465010.23869/bphjbr.21.1.20152Screening of rapd primer for teak (Tectona grandis)Imas Cintamulya0University of PGRI RonggolaweIdentification of DNA polymorphisms in teak is important. It is a first step to determine the presence of genetic varia-tion in teak. The information of genetic variation is needed for teak breeding development. RAPD is one of method which can be used for identification of DNA polymorphism. This study aim to get the RAPD primer which can detect the DNA polymorphism in teak. Benefits of this study are provide information about primer which can detect the DNA polymorphism in teak, DNA polymorphism data can be used for genetic variation analysis which needed for teak breeding development. The primers which used in this study shown the DNA polymorphism in teak. The primer are OPF6 (5'-GGGAATTCGG-3 '), OPF8 (5'-GGGATATCGC-3'), and OPF11 (5'-TTGGTACCCC-3 '). The highest DNA poly-morphism is shown in DNA which amplified with OPF-8 primer. Keywords: RAPD, Primer, Polymorphism, DNA, Tectona grandishttp://berkalahayati.org/journal/10f760a8molecular biologyplant diversity |
| spellingShingle | Imas Cintamulya Screening of rapd primer for teak (Tectona grandis) Journal of Biological Researches molecular biology plant diversity |
| title | Screening of rapd primer for teak (Tectona grandis) |
| title_full | Screening of rapd primer for teak (Tectona grandis) |
| title_fullStr | Screening of rapd primer for teak (Tectona grandis) |
| title_full_unstemmed | Screening of rapd primer for teak (Tectona grandis) |
| title_short | Screening of rapd primer for teak (Tectona grandis) |
| title_sort | screening of rapd primer for teak tectona grandis |
| topic | molecular biology plant diversity |
| url | http://berkalahayati.org/journal/10f760a8 |
| work_keys_str_mv | AT imascintamulya screeningofrapdprimerforteaktectonagrandis |