“Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing
Prussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the con...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/6/193 |
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| author | Galina Z. Gayda Olha M. Demkiv Yanna Gurianov Roman Ya. Serkiz Halyna M. Klepach Mykhailo V. Gonchar Marina Nisnevitch |
| author_facet | Galina Z. Gayda Olha M. Demkiv Yanna Gurianov Roman Ya. Serkiz Halyna M. Klepach Mykhailo V. Gonchar Marina Nisnevitch |
| author_sort | Galina Z. Gayda |
| collection | DOAJ |
| description | Prussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the construction of biosensors and biofuel cells. The “green” synthesis of PO-like PBAs using oxido-reductase flavocytochrome <i>b</i><sub>2</sub> is described in this study. When immobilized on graphite electrodes (GEs), the obtained green-synthesized PBAs or hexacyanoferrates (gHCFs) of transition and noble metals produced amperometric signals in response to H<sub>2</sub>O<sub>2</sub>. HCFs of copper, iron, palladium and other metals were synthesized and characterized by structure, size, catalytic properties and electro-mediator activities. The gCuHCF, as the most effective PO mimetic with a flower-like micro/nano superstructure, was used as an H<sub>2</sub>O<sub>2</sub>-sensitive platform for the development of a glucose oxidase (GO)-based biosensor. The GO/gCuHCF/GE biosensor exhibited high sensitivity (710 A M<sup>−1</sup>m<sup>−2</sup>), a broad linear range and good selectivity when tested on real samples of fruit juices. We propose that the gCuHCF and other gHCFs synthesized via enzymes may be used as artificial POs in amperometric oxidase-based (bio)sensors. |
| first_indexed | 2024-03-10T10:32:10Z |
| format | Article |
| id | doaj.art-59882a0f85904a8f954a4f210dd4e571 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T10:32:10Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-59882a0f85904a8f954a4f210dd4e5712023-11-21T23:35:07ZengMDPI AGBiosensors2079-63742021-06-0111619310.3390/bios11060193“Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and BiosensingGalina Z. Gayda0Olha M. Demkiv1Yanna Gurianov2Roman Ya. Serkiz3Halyna M. Klepach4Mykhailo V. Gonchar5Marina Nisnevitch6Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, UkraineInstitute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, UkraineDepartment of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, IsraelInstitute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, UkraineFaculty of Biology and Natural Sciences, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, UkraineInstitute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, UkraineDepartment of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, IsraelPrussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the construction of biosensors and biofuel cells. The “green” synthesis of PO-like PBAs using oxido-reductase flavocytochrome <i>b</i><sub>2</sub> is described in this study. When immobilized on graphite electrodes (GEs), the obtained green-synthesized PBAs or hexacyanoferrates (gHCFs) of transition and noble metals produced amperometric signals in response to H<sub>2</sub>O<sub>2</sub>. HCFs of copper, iron, palladium and other metals were synthesized and characterized by structure, size, catalytic properties and electro-mediator activities. The gCuHCF, as the most effective PO mimetic with a flower-like micro/nano superstructure, was used as an H<sub>2</sub>O<sub>2</sub>-sensitive platform for the development of a glucose oxidase (GO)-based biosensor. The GO/gCuHCF/GE biosensor exhibited high sensitivity (710 A M<sup>−1</sup>m<sup>−2</sup>), a broad linear range and good selectivity when tested on real samples of fruit juices. We propose that the gCuHCF and other gHCFs synthesized via enzymes may be used as artificial POs in amperometric oxidase-based (bio)sensors.https://www.mdpi.com/2079-6374/11/6/193artificial enzymesgreen synthesishexacyanoferrates of transition and noble metalsperoxidase mimeticamperometric (bio)sensorglucose oxidase |
| spellingShingle | Galina Z. Gayda Olha M. Demkiv Yanna Gurianov Roman Ya. Serkiz Halyna M. Klepach Mykhailo V. Gonchar Marina Nisnevitch “Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing Biosensors artificial enzymes green synthesis hexacyanoferrates of transition and noble metals peroxidase mimetic amperometric (bio)sensor glucose oxidase |
| title | “Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing |
| title_full | “Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing |
| title_fullStr | “Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing |
| title_full_unstemmed | “Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing |
| title_short | “Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing |
| title_sort | green prussian blue analogues as peroxidase mimetics for amperometric sensing and biosensing |
| topic | artificial enzymes green synthesis hexacyanoferrates of transition and noble metals peroxidase mimetic amperometric (bio)sensor glucose oxidase |
| url | https://www.mdpi.com/2079-6374/11/6/193 |
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