Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter
We herein describe a cascade enzymatic reaction (CER)-based IgE detection method utilizing a personal glucose meter (PGM), which relies on alkaline phosphatase (ALP) activity that regulates the amount of adenosine triphosphate (ATP). The amount of sandwich assay complex is determined according to th...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/19/6396 |
| _version_ | 1797515775913754624 |
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| author | Hyogu Han Junhyun Park Jun Ki Ahn |
| author_facet | Hyogu Han Junhyun Park Jun Ki Ahn |
| author_sort | Hyogu Han |
| collection | DOAJ |
| description | We herein describe a cascade enzymatic reaction (CER)-based IgE detection method utilizing a personal glucose meter (PGM), which relies on alkaline phosphatase (ALP) activity that regulates the amount of adenosine triphosphate (ATP). The amount of sandwich assay complex is determined according to the presence or absence of the target IgE. Additionally, the ALP in the sandwich assay catalyzes the dephosphorylation of ATP, a substrate of CER, which results in the changes in glucose level. By employing this principle, IgE was reliably detected at a concentration as low as ca. 29.6 ng/mL with high specificity toward various proteins. Importantly, the limit of detection (LOD) of this portable PGM-based approach was comparable to currently commercialized ELISA kit without expensive and bulky analysis equipment as well as complexed washing step. Finally, the diagnostic capability of this method was also successfully verified by reliably detecting IgE present in a real human serum sample with an excellent recovery ratio within 100 ± 6%. |
| first_indexed | 2024-03-10T06:52:03Z |
| format | Article |
| id | doaj.art-5989fb3e3a4644fa92c5652f6f082f39 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T06:52:03Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-5989fb3e3a4644fa92c5652f6f082f392023-11-22T16:45:26ZengMDPI AGSensors1424-82202021-09-012119639610.3390/s21196396Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose MeterHyogu Han0Junhyun Park1Jun Ki Ahn2Material & Component Convergence R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, KoreaMaterial & Component Convergence R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, KoreaMaterial & Component Convergence R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, KoreaWe herein describe a cascade enzymatic reaction (CER)-based IgE detection method utilizing a personal glucose meter (PGM), which relies on alkaline phosphatase (ALP) activity that regulates the amount of adenosine triphosphate (ATP). The amount of sandwich assay complex is determined according to the presence or absence of the target IgE. Additionally, the ALP in the sandwich assay catalyzes the dephosphorylation of ATP, a substrate of CER, which results in the changes in glucose level. By employing this principle, IgE was reliably detected at a concentration as low as ca. 29.6 ng/mL with high specificity toward various proteins. Importantly, the limit of detection (LOD) of this portable PGM-based approach was comparable to currently commercialized ELISA kit without expensive and bulky analysis equipment as well as complexed washing step. Finally, the diagnostic capability of this method was also successfully verified by reliably detecting IgE present in a real human serum sample with an excellent recovery ratio within 100 ± 6%.https://www.mdpi.com/1424-8220/21/19/6396immunoglobulin Ealkaline phosphatasecascade enzymatic reactionpersonal glucose meterbiosensor |
| spellingShingle | Hyogu Han Junhyun Park Jun Ki Ahn Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter Sensors immunoglobulin E alkaline phosphatase cascade enzymatic reaction personal glucose meter biosensor |
| title | Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter |
| title_full | Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter |
| title_fullStr | Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter |
| title_full_unstemmed | Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter |
| title_short | Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter |
| title_sort | immunoglobulin e detection method based on cascade enzymatic reaction utilizing portable personal glucose meter |
| topic | immunoglobulin E alkaline phosphatase cascade enzymatic reaction personal glucose meter biosensor |
| url | https://www.mdpi.com/1424-8220/21/19/6396 |
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