PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells.
Mechanically stressed vascular smooth muscle cells (VSMCs) have potential roles in the development of vascular complications. However, the underlying mechanisms are unclear. Using VSMCs cultured from rat thoracic aorta explants, we investigated the effects of mechanical stretch (MS) on the cellular...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0265191 |
| _version_ | 1818030004450099200 |
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| author | Ji On Kim Seung Eun Baek Eun Yeong Jeon Jong Min Choi Eun Jeong Jang Chi Dae Kim |
| author_facet | Ji On Kim Seung Eun Baek Eun Yeong Jeon Jong Min Choi Eun Jeong Jang Chi Dae Kim |
| author_sort | Ji On Kim |
| collection | DOAJ |
| description | Mechanically stressed vascular smooth muscle cells (VSMCs) have potential roles in the development of vascular complications. However, the underlying mechanisms are unclear. Using VSMCs cultured from rat thoracic aorta explants, we investigated the effects of mechanical stretch (MS) on the cellular secretion of high mobility group box 1 (HMGB1), a major damage-associated molecular pattern that mediates vascular complications in stressed vasculature. Enzyme-linked immunosorbent assay (ELISA) demonstrated an increase in the secretion of HMGB1 in VSMCs stimulated with MS (0-3% strain, 60 cycles/min), and this secretion was markedly and time-dependently increased at 3% MS. The increased secretion of HMGB1 at 3% MS was accompanied by an increased cytosolic translocation of nuclear HMGB1; the acetylated and phosphorylated forms of this protein were significantly increased. Among various inhibitors of membrane receptors mediating mechanical signals, AG1295 (a platelet-derived growth factor receptor (PDGFR) inhibitor) attenuated MS-induced HMGB1 secretion. Inhibitors of other receptors, including epidermal growth factor, insulin-like growth factor, and fibroblast growth factor receptors, did not inhibit this secretion. Additionally, MS-induced HMGB1 secretion was markedly attenuated in PDGFR-β-deficient cells but not in cells transfected with PDGFR-α siRNA. Likewise, PDGF-DD, but not PDGF-AA, directly increased HMGB1 secretion in VSMCs, indicating a pivotal role of PDGFR-β signaling in the secretion of this protein in VSMCs. Thus, targeting PDGFR-β-mediated secretion of HMGB1 in VSMCs might be a promising therapeutic strategy for vascular complications associated with hypertension. |
| first_indexed | 2024-12-10T05:28:41Z |
| format | Article |
| id | doaj.art-599076981aee4c6a91573f44efd1ee94 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:28:41Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-599076981aee4c6a91573f44efd1ee942022-12-22T02:00:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173e026519110.1371/journal.pone.0265191PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells.Ji On KimSeung Eun BaekEun Yeong JeonJong Min ChoiEun Jeong JangChi Dae KimMechanically stressed vascular smooth muscle cells (VSMCs) have potential roles in the development of vascular complications. However, the underlying mechanisms are unclear. Using VSMCs cultured from rat thoracic aorta explants, we investigated the effects of mechanical stretch (MS) on the cellular secretion of high mobility group box 1 (HMGB1), a major damage-associated molecular pattern that mediates vascular complications in stressed vasculature. Enzyme-linked immunosorbent assay (ELISA) demonstrated an increase in the secretion of HMGB1 in VSMCs stimulated with MS (0-3% strain, 60 cycles/min), and this secretion was markedly and time-dependently increased at 3% MS. The increased secretion of HMGB1 at 3% MS was accompanied by an increased cytosolic translocation of nuclear HMGB1; the acetylated and phosphorylated forms of this protein were significantly increased. Among various inhibitors of membrane receptors mediating mechanical signals, AG1295 (a platelet-derived growth factor receptor (PDGFR) inhibitor) attenuated MS-induced HMGB1 secretion. Inhibitors of other receptors, including epidermal growth factor, insulin-like growth factor, and fibroblast growth factor receptors, did not inhibit this secretion. Additionally, MS-induced HMGB1 secretion was markedly attenuated in PDGFR-β-deficient cells but not in cells transfected with PDGFR-α siRNA. Likewise, PDGF-DD, but not PDGF-AA, directly increased HMGB1 secretion in VSMCs, indicating a pivotal role of PDGFR-β signaling in the secretion of this protein in VSMCs. Thus, targeting PDGFR-β-mediated secretion of HMGB1 in VSMCs might be a promising therapeutic strategy for vascular complications associated with hypertension.https://doi.org/10.1371/journal.pone.0265191 |
| spellingShingle | Ji On Kim Seung Eun Baek Eun Yeong Jeon Jong Min Choi Eun Jeong Jang Chi Dae Kim PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells. PLoS ONE |
| title | PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells. |
| title_full | PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells. |
| title_fullStr | PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells. |
| title_full_unstemmed | PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells. |
| title_short | PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells. |
| title_sort | pdgfr β signaling mediates hmgb1 release in mechanically stressed vascular smooth muscle cells |
| url | https://doi.org/10.1371/journal.pone.0265191 |
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