Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA
Deletions within genes coding for subunits of the transcription coactivator SAGA caused strong genome-wide defects in transcription and SAGA-mediated chromatin modifications. In contrast, rapid SAGA depletion produced only modest transcription defects at 13% of protein-coding genes – genes that are...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/50109 |
| _version_ | 1828374501333663744 |
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| author | Rafal Donczew Linda Warfield Derek Pacheco Ariel Erijman Steven Hahn |
| author_facet | Rafal Donczew Linda Warfield Derek Pacheco Ariel Erijman Steven Hahn |
| author_sort | Rafal Donczew |
| collection | DOAJ |
| description | Deletions within genes coding for subunits of the transcription coactivator SAGA caused strong genome-wide defects in transcription and SAGA-mediated chromatin modifications. In contrast, rapid SAGA depletion produced only modest transcription defects at 13% of protein-coding genes – genes that are generally more sensitive to rapid TFIID depletion. However, transcription of these ‘coactivator-redundant’ genes is strongly affected by rapid depletion of both factors, showing the overlapping functions of TFIID and SAGA at this gene set. We suggest that this overlapping function is linked to TBP-DNA recruitment. The remaining 87% of expressed genes that we term ‘TFIID-dependent’ are highly sensitive to rapid TFIID depletion and insensitive to rapid SAGA depletion. Genome-wide mapping of SAGA and TFIID found binding of both factors at many genes independent of gene class. Promoter analysis suggests that the distinction between the gene classes is due to multiple components rather than any single regulatory factor or promoter sequence motif. |
| first_indexed | 2024-04-14T07:33:54Z |
| format | Article |
| id | doaj.art-5998dd751ff940d4915119fce8299ea6 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:33:54Z |
| publishDate | 2020-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5998dd751ff940d4915119fce8299ea62022-12-22T02:05:44ZengeLife Sciences Publications LtdeLife2050-084X2020-01-01910.7554/eLife.50109Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGARafal Donczew0https://orcid.org/0000-0001-9729-4153Linda Warfield1Derek Pacheco2Ariel Erijman3Steven Hahn4https://orcid.org/0000-0001-7240-2533Fred Hutchinson Cancer Research Center, Seattle, United StatesFred Hutchinson Cancer Research Center, Seattle, United StatesFred Hutchinson Cancer Research Center, Seattle, United StatesFred Hutchinson Cancer Research Center, Seattle, United StatesFred Hutchinson Cancer Research Center, Seattle, United StatesDeletions within genes coding for subunits of the transcription coactivator SAGA caused strong genome-wide defects in transcription and SAGA-mediated chromatin modifications. In contrast, rapid SAGA depletion produced only modest transcription defects at 13% of protein-coding genes – genes that are generally more sensitive to rapid TFIID depletion. However, transcription of these ‘coactivator-redundant’ genes is strongly affected by rapid depletion of both factors, showing the overlapping functions of TFIID and SAGA at this gene set. We suggest that this overlapping function is linked to TBP-DNA recruitment. The remaining 87% of expressed genes that we term ‘TFIID-dependent’ are highly sensitive to rapid TFIID depletion and insensitive to rapid SAGA depletion. Genome-wide mapping of SAGA and TFIID found binding of both factors at many genes independent of gene class. Promoter analysis suggests that the distinction between the gene classes is due to multiple components rather than any single regulatory factor or promoter sequence motif.https://elifesciences.org/articles/50109transcriptioncoactivatorTFIIDSAGAchromatin |
| spellingShingle | Rafal Donczew Linda Warfield Derek Pacheco Ariel Erijman Steven Hahn Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA eLife transcription coactivator TFIID SAGA chromatin |
| title | Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA |
| title_full | Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA |
| title_fullStr | Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA |
| title_full_unstemmed | Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA |
| title_short | Two roles for the yeast transcription coactivator SAGA and a set of genes redundantly regulated by TFIID and SAGA |
| title_sort | two roles for the yeast transcription coactivator saga and a set of genes redundantly regulated by tfiid and saga |
| topic | transcription coactivator TFIID SAGA chromatin |
| url | https://elifesciences.org/articles/50109 |
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