Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro
Callus cultures from cotyledon and leaf explants of a Spanish cultivar of melon (Amarillo Oro) were tested for growth and morphogenic capacity on several culture media with different concentrations of IAA (indole-3-acetic acid) in combination with 1.0 mg.L-1 BA (6-benzylaminopurine) or 6.0 mg.L-1 KI...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2006-01-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100003 |
| _version_ | 1818304758508683264 |
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| author | Fernanda Vidigal Duarte Souza Begoña Garcia-Sogo Antonio da Silva Souza Amparo Pérez San-Juán Vicente Moreno |
| author_facet | Fernanda Vidigal Duarte Souza Begoña Garcia-Sogo Antonio da Silva Souza Amparo Pérez San-Juán Vicente Moreno |
| author_sort | Fernanda Vidigal Duarte Souza |
| collection | DOAJ |
| description | Callus cultures from cotyledon and leaf explants of a Spanish cultivar of melon (Amarillo Oro) were tested for growth and morphogenic capacity on several culture media with different concentrations of IAA (indole-3-acetic acid) in combination with 1.0 mg.L-1 BA (6-benzylaminopurine) or 6.0 mg.L-1 KIN (kinetin). The best results were achieved with cotyledon explants. The leaf explants presented low bud formation capacity. Variability of organogenic response on cotyledons of different age (7, 5, 3 and 1-day-old) was evaluated. The age of explant had a significant influence on bud induction. Cotyledon explants from 7-day-old seedlings showed higher organogenic index and development of shoots when cultured onto MS medium supplemented with 1.5 mg.L-1 of IAA and 1.0 mg.L-1 of BA. The effect of cut type of cotyledonary explants on organogenic response was also investigated. Explants cut transversally showed the best results. The addition of copper sulfate in the culture medium promoted a qualitative improvement of the regenerated shoots.<br>Explantes de cotilédones e folhas do cultivar de melão Amarillo Oro foram cultivados para avaliação do potencial morfogenético em diferentes meios de cultura com diversas concentrações de ácido indolacético (AIA), em combinação com 1,0 mg.L-1 benziladenina (BA) e 6,0 mg.L-1 de cinetina (CIN). Os melhores resultados foram obtidos com explantes de cotilédones, sendo que explantes de folhas mostraram uma capacidade baixa na indução de gemas. A variação na resposta organogênica em cotilédones de diferentes idades fisiológicas (1, 3, 5 e 7 dias após a germinação) mostraram que os melhores resultados foram obtidos com cotilédones de sete dias cultivados no meio MS suplementado com 1,5 mg.L-1 de AIA e 1,0 mg.L-1 de BA. O efeito do tipo de corte na resposta organogênica de explantes cotiledonares foi também avaliado. Os melhores resultados foram obtidos com explantes cortados transversalmente. Adição suplementar de sulfato de cobre ao meio de cultura resultou em uma melhora na qualidade da dos brotos regenerados. |
| first_indexed | 2024-12-13T06:15:47Z |
| format | Article |
| id | doaj.art-599b1ff1b9a040c2917673e2abd5a67a |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-13T06:15:47Z |
| publishDate | 2006-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-599b1ff1b9a040c2917673e2abd5a67a2022-12-21T23:56:58ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242006-01-01491212710.1590/S1516-89132006000100003Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo OroFernanda Vidigal Duarte SouzaBegoña Garcia-SogoAntonio da Silva SouzaAmparo Pérez San-JuánVicente MorenoCallus cultures from cotyledon and leaf explants of a Spanish cultivar of melon (Amarillo Oro) were tested for growth and morphogenic capacity on several culture media with different concentrations of IAA (indole-3-acetic acid) in combination with 1.0 mg.L-1 BA (6-benzylaminopurine) or 6.0 mg.L-1 KIN (kinetin). The best results were achieved with cotyledon explants. The leaf explants presented low bud formation capacity. Variability of organogenic response on cotyledons of different age (7, 5, 3 and 1-day-old) was evaluated. The age of explant had a significant influence on bud induction. Cotyledon explants from 7-day-old seedlings showed higher organogenic index and development of shoots when cultured onto MS medium supplemented with 1.5 mg.L-1 of IAA and 1.0 mg.L-1 of BA. The effect of cut type of cotyledonary explants on organogenic response was also investigated. Explants cut transversally showed the best results. The addition of copper sulfate in the culture medium promoted a qualitative improvement of the regenerated shoots.<br>Explantes de cotilédones e folhas do cultivar de melão Amarillo Oro foram cultivados para avaliação do potencial morfogenético em diferentes meios de cultura com diversas concentrações de ácido indolacético (AIA), em combinação com 1,0 mg.L-1 benziladenina (BA) e 6,0 mg.L-1 de cinetina (CIN). Os melhores resultados foram obtidos com explantes de cotilédones, sendo que explantes de folhas mostraram uma capacidade baixa na indução de gemas. A variação na resposta organogênica em cotilédones de diferentes idades fisiológicas (1, 3, 5 e 7 dias após a germinação) mostraram que os melhores resultados foram obtidos com cotilédones de sete dias cultivados no meio MS suplementado com 1,5 mg.L-1 de AIA e 1,0 mg.L-1 de BA. O efeito do tipo de corte na resposta organogênica de explantes cotiledonares foi também avaliado. Os melhores resultados foram obtidos com explantes cortados transversalmente. Adição suplementar de sulfato de cobre ao meio de cultura resultou em uma melhora na qualidade da dos brotos regenerados.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100003Organogenesisplant regenerationleaf explantcotyledon explantcucurbitaceae |
| spellingShingle | Fernanda Vidigal Duarte Souza Begoña Garcia-Sogo Antonio da Silva Souza Amparo Pérez San-Juán Vicente Moreno Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro Brazilian Archives of Biology and Technology Organogenesis plant regeneration leaf explant cotyledon explant cucurbitaceae |
| title | Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro |
| title_full | Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro |
| title_fullStr | Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro |
| title_full_unstemmed | Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro |
| title_short | Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro |
| title_sort | morphogenetic response of cotyledon and leaf explants of melon cucumis melo l cv amarillo oro |
| topic | Organogenesis plant regeneration leaf explant cotyledon explant cucurbitaceae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100003 |
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