OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes
A novel gas chromatography method was developed using automatic injections to identify and quantify the amount of residual solvents or analytes in samples of fluorine-18 and carbon-11 radiopharmaceuticals. This approach evaluates seven analytes in less than 5 versus 13 min of acquisition time. The m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/11/1623 |
| _version_ | 1797458038348578816 |
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| author | Camry Vonyae’ A’Keen Jakub Mroz Simon Kunta Joseph Jairo Baquero Melchor V. Cantorias Patrick Carberry |
| author_facet | Camry Vonyae’ A’Keen Jakub Mroz Simon Kunta Joseph Jairo Baquero Melchor V. Cantorias Patrick Carberry |
| author_sort | Camry Vonyae’ A’Keen |
| collection | DOAJ |
| description | A novel gas chromatography method was developed using automatic injections to identify and quantify the amount of residual solvents or analytes in samples of fluorine-18 and carbon-11 radiopharmaceuticals. This approach evaluates seven analytes in less than 5 versus 13 min of acquisition time. The method additionally includes a 3 min bakeout to aid in the removal and carry-over of higher-boiling impurities. Chromatographic parameters such as column temperature, hold time, column pressure, flow rate, and split ratios were adjusted and optimized to analyze radioactive drug samples containing analytes which include methanol, ethanol, acetone, acetonitrile, triethylamine, <i>N</i>,<i>N</i>-dimethylformamide, and dimethyl sulfoxide. The relative standard deviation for each solvent was determined to be no greater than 1.6%. The method limit of detection (LOD) and limit of quantification (LOQ) were between 0.053 and 0.163 and 0.000 (5.791 × 10<sup>−6</sup>) and 0.520 mg/mL, respectively. This GC technique, using flame ionization detection (FID), was validated and is currently employed for the routine quality control of all approved IND and RDRC PET radiopharmaceuticals at our center. |
| first_indexed | 2024-03-09T16:32:15Z |
| format | Article |
| id | doaj.art-599eaded7a634e5499481305307ff800 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T16:32:15Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-599eaded7a634e5499481305307ff8002023-11-24T15:00:37ZengMDPI AGPharmaceuticals1424-82472023-11-011611162310.3390/ph16111623OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 MinutesCamry Vonyae’ A’Keen0Jakub Mroz1Simon Kunta Joseph2Jairo Baquero3Melchor V. Cantorias4Patrick Carberry5Department of Radiology, New York University Grossman School of Medicine, 660 First Avenue, Room 240, New York, NY 10016, USADepartment of Radiology, New York University Grossman School of Medicine, 660 First Avenue, Room 240, New York, NY 10016, USADepartment of Radiology, New York University Grossman School of Medicine, 660 First Avenue, Room 240, New York, NY 10016, USADepartment of Radiology, New York University Grossman School of Medicine, 660 First Avenue, Room 240, New York, NY 10016, USADepartment of Radiology, New York University Grossman School of Medicine, 660 First Avenue, Room 240, New York, NY 10016, USADepartment of Radiology, New York University Grossman School of Medicine, 660 First Avenue, Room 240, New York, NY 10016, USAA novel gas chromatography method was developed using automatic injections to identify and quantify the amount of residual solvents or analytes in samples of fluorine-18 and carbon-11 radiopharmaceuticals. This approach evaluates seven analytes in less than 5 versus 13 min of acquisition time. The method additionally includes a 3 min bakeout to aid in the removal and carry-over of higher-boiling impurities. Chromatographic parameters such as column temperature, hold time, column pressure, flow rate, and split ratios were adjusted and optimized to analyze radioactive drug samples containing analytes which include methanol, ethanol, acetone, acetonitrile, triethylamine, <i>N</i>,<i>N</i>-dimethylformamide, and dimethyl sulfoxide. The relative standard deviation for each solvent was determined to be no greater than 1.6%. The method limit of detection (LOD) and limit of quantification (LOQ) were between 0.053 and 0.163 and 0.000 (5.791 × 10<sup>−6</sup>) and 0.520 mg/mL, respectively. This GC technique, using flame ionization detection (FID), was validated and is currently employed for the routine quality control of all approved IND and RDRC PET radiopharmaceuticals at our center.https://www.mdpi.com/1424-8247/16/11/1623gas chromatographyanalytespositron emission tomographyradiotracerquality controlcarbon-11 |
| spellingShingle | Camry Vonyae’ A’Keen Jakub Mroz Simon Kunta Joseph Jairo Baquero Melchor V. Cantorias Patrick Carberry OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes Pharmaceuticals gas chromatography analytes positron emission tomography radiotracer quality control carbon-11 |
| title | OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes |
| title_full | OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes |
| title_fullStr | OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes |
| title_full_unstemmed | OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes |
| title_short | OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes |
| title_sort | omni gas chromatograph captures seven common pet radiotracer analytes in under 5 minutes |
| topic | gas chromatography analytes positron emission tomography radiotracer quality control carbon-11 |
| url | https://www.mdpi.com/1424-8247/16/11/1623 |
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