Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts
Objective: To screen the cytotoxic effects of some marine sponges extracts on HeLa and PC12 cells. Methods: Five marine sponges including Ircinia echinata (I. echinata), Dysidea avara, Axinella sinoxea, Haliclona tubifera and Haliclona violacea were collected from the Persian Gulf (Hengam Island). T...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-11-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115001860 |
| _version_ | 1811310596045406208 |
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| author | Davood Mahdian Milad Iranshahy Abolfazl Shakeri Azar Hoseini Hoda Yavari Melika Nazemi Mehrdad Iranshahi |
| author_facet | Davood Mahdian Milad Iranshahy Abolfazl Shakeri Azar Hoseini Hoda Yavari Melika Nazemi Mehrdad Iranshahi |
| author_sort | Davood Mahdian |
| collection | DOAJ |
| description | Objective: To screen the cytotoxic effects of some marine sponges extracts on HeLa and PC12 cells.
Methods: Five marine sponges including Ircinia echinata (I. echinata), Dysidea avara, Axinella sinoxea, Haliclona tubifera and Haliclona violacea were collected from the Persian Gulf (Hengam Island). The cytotoxic effect of these sponges was evaluated by using MTT assay. The metabolic high performance liquid chromatography fingerprint of I. echinata was also carried out at two wavelengths (254 and 280 nm).
Results: Among the sponges tested in this study, the extracts of I. echinata and Dysidea avara possessed the cytotoxic effect on HeLa and PC12 cells. The obtained fractions from high performance liquid chromatography were evaluated for their cytotoxic properties against the cell lines. The isolated fractions did not show significant cytotoxic properties.
Conclusions: I. echinata could be considered as a potential extract for chemotherapy. Further investigation is needed to determine the accuracy of mechanism. |
| first_indexed | 2024-04-13T10:02:59Z |
| format | Article |
| id | doaj.art-59a201ed37c942488369c93e532fdb76 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-04-13T10:02:59Z |
| publishDate | 2015-11-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-59a201ed37c942488369c93e532fdb762022-12-22T02:51:12ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-11-0151189690110.1016/j.apjtb.2015.07.020Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extractsDavood Mahdian0Milad Iranshahy1Abolfazl Shakeri2Azar Hoseini3Hoda Yavari4Melika Nazemi5Mehrdad Iranshahi6Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranPharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranPersian Gulf and Oman Sea Ecological Research Organization, Iranian Fisheries Research Organization, Bandar Abbas, IranBiotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranObjective: To screen the cytotoxic effects of some marine sponges extracts on HeLa and PC12 cells. Methods: Five marine sponges including Ircinia echinata (I. echinata), Dysidea avara, Axinella sinoxea, Haliclona tubifera and Haliclona violacea were collected from the Persian Gulf (Hengam Island). The cytotoxic effect of these sponges was evaluated by using MTT assay. The metabolic high performance liquid chromatography fingerprint of I. echinata was also carried out at two wavelengths (254 and 280 nm). Results: Among the sponges tested in this study, the extracts of I. echinata and Dysidea avara possessed the cytotoxic effect on HeLa and PC12 cells. The obtained fractions from high performance liquid chromatography were evaluated for their cytotoxic properties against the cell lines. The isolated fractions did not show significant cytotoxic properties. Conclusions: I. echinata could be considered as a potential extract for chemotherapy. Further investigation is needed to determine the accuracy of mechanism.http://www.sciencedirect.com/science/article/pii/S2221169115001860Marine spongesCytotoxicityHeLaPC12MTT test |
| spellingShingle | Davood Mahdian Milad Iranshahy Abolfazl Shakeri Azar Hoseini Hoda Yavari Melika Nazemi Mehrdad Iranshahi Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts Asian Pacific Journal of Tropical Biomedicine Marine sponges Cytotoxicity HeLa PC12 MTT test |
| title | Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts |
| title_full | Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts |
| title_fullStr | Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts |
| title_full_unstemmed | Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts |
| title_short | Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts |
| title_sort | cytotoxicity evaluation of extracts and fractions of five marine sponges from the persian gulf and hplc fingerprint analysis of cytotoxic extracts |
| topic | Marine sponges Cytotoxicity HeLa PC12 MTT test |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115001860 |
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