Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report
ABSTRACT Leishmaniasis is a parasitic disease of worldwide spread. It is caused by protozoa of the genus Leishmania and is transmitted to animals and humans through the bite of sand flies. In Brazil, leishmaniasis is one of the zoonoses of major importance and expansion. The objective of this work i...
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| Format: | Article |
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Universidade Federal de Minas Gerais
2020-07-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000300744&tlng=en |
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| author | L. Dalla Rosa A.G. Soares A. Marcili J.D.S. Diaz P. Wolkmer D.M. Bassuino P.G.J. Schaefer K. Henrich C.K. Rossato |
| author_facet | L. Dalla Rosa A.G. Soares A. Marcili J.D.S. Diaz P. Wolkmer D.M. Bassuino P.G.J. Schaefer K. Henrich C.K. Rossato |
| author_sort | L. Dalla Rosa |
| collection | DOAJ |
| description | ABSTRACT Leishmaniasis is a parasitic disease of worldwide spread. It is caused by protozoa of the genus Leishmania and is transmitted to animals and humans through the bite of sand flies. In Brazil, leishmaniasis is one of the zoonoses of major importance and expansion. The objective of this work is to describe the clinical, pathological, immunohistochemical and molecular findings of cutaneous leishmaniasis by Leishmania enriettii in guinea pig (Cavia porcellus). Three animals had nodular and alopecia lesions on the muzzle, ears and ulcerated lesions on the distal extremities of the pelvic limbs. The males (2) also had diffuse thickening of the scrotal skin. Samples of the ulcerated cutaneous lesions were evaluated by cytology which were observed as amastigote forms of Leishmania. One of the animals was euthanized and necropsied. Histopathology showed abundant dermal infiltrate of macrophages, plasma cells, lymphocytes and multinucleated giant cells. Numerous macrophages contained parasitoid vacuoles with amastigote forms, evidenced by immunohistochemical examination. The molecular characterization based on the SSUrDNA gene identified the species as L. enrietti. The diagnosis of cutaneous leishmaniasis in these cases was based on pathological findings and confirmed by immunohistochemistry, PCR and sequencing. |
| first_indexed | 2024-04-11T17:14:41Z |
| format | Article |
| id | doaj.art-59a39b3cf6554a5bb7d324cec1fd2aba |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-04-11T17:14:41Z |
| publishDate | 2020-07-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-59a39b3cf6554a5bb7d324cec1fd2aba2022-12-22T04:12:45ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622020-07-0172374474810.1590/1678-4162-11459Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case reportL. Dalla Rosahttps://orcid.org/0000-0002-5047-0014A.G. Soareshttps://orcid.org/0000-0002-8250-0329A. Marcilihttps://orcid.org/0000-0002-0478-6771J.D.S. Diazhttps://orcid.org/0000-0002-6530-2419P. Wolkmerhttps://orcid.org/0000-0001-6474-0102D.M. Bassuinohttps://orcid.org/0000-0002-7724-3639P.G.J. Schaeferhttps://orcid.org/0000-0002-4395-0933K. Henrichhttps://orcid.org/0000-0002-5864-025XC.K. Rossatohttps://orcid.org/0000-0002-8401-2759ABSTRACT Leishmaniasis is a parasitic disease of worldwide spread. It is caused by protozoa of the genus Leishmania and is transmitted to animals and humans through the bite of sand flies. In Brazil, leishmaniasis is one of the zoonoses of major importance and expansion. The objective of this work is to describe the clinical, pathological, immunohistochemical and molecular findings of cutaneous leishmaniasis by Leishmania enriettii in guinea pig (Cavia porcellus). Three animals had nodular and alopecia lesions on the muzzle, ears and ulcerated lesions on the distal extremities of the pelvic limbs. The males (2) also had diffuse thickening of the scrotal skin. Samples of the ulcerated cutaneous lesions were evaluated by cytology which were observed as amastigote forms of Leishmania. One of the animals was euthanized and necropsied. Histopathology showed abundant dermal infiltrate of macrophages, plasma cells, lymphocytes and multinucleated giant cells. Numerous macrophages contained parasitoid vacuoles with amastigote forms, evidenced by immunohistochemical examination. The molecular characterization based on the SSUrDNA gene identified the species as L. enrietti. The diagnosis of cutaneous leishmaniasis in these cases was based on pathological findings and confirmed by immunohistochemistry, PCR and sequencing.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000300744&tlng=enLeishmania enriettiiprotozoanzoonosis |
| spellingShingle | L. Dalla Rosa A.G. Soares A. Marcili J.D.S. Diaz P. Wolkmer D.M. Bassuino P.G.J. Schaefer K. Henrich C.K. Rossato Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report Arquivo Brasileiro de Medicina Veterinária e Zootecnia Leishmania enriettii protozoan zoonosis |
| title | Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report |
| title_full | Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report |
| title_fullStr | Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report |
| title_full_unstemmed | Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report |
| title_short | Cutaneous leishmaniasis in Cavia porcellus (guinea pig): case report |
| title_sort | cutaneous leishmaniasis in cavia porcellus guinea pig case report |
| topic | Leishmania enriettii protozoan zoonosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000300744&tlng=en |
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