Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis
Reference methods for antifungal susceptibility testing of yeasts have been developed by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). These methods are intended to test the main pathogenic yeasts that cause invasive...
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| Format: | Article |
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MDPI AG
2017-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/3/3/37 |
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| author | Andrea Peano Mario Pasquetti Paolo Tizzani Elisa Chiavassa Jacques Guillot Elizabeth Johnson |
| author_facet | Andrea Peano Mario Pasquetti Paolo Tizzani Elisa Chiavassa Jacques Guillot Elizabeth Johnson |
| author_sort | Andrea Peano |
| collection | DOAJ |
| description | Reference methods for antifungal susceptibility testing of yeasts have been developed by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). These methods are intended to test the main pathogenic yeasts that cause invasive infections, namely Candida spp. and Cryptococcus neoformans, while testing other yeast species introduces several additional problems in standardization not addressed by these reference procedures. As a consequence, a number of procedures have been employed in the literature to test the antifungal susceptibility of Malassezia pachydermatis. This has resulted in conflicting results. The aim of the present study is to review the procedures and the technical parameters (growth media, inoculum preparation, temperature and length of incubation, method of reading) employed for susceptibility testing of M. pachydermatis, and when possible, to propose recommendations for or against their use. Such information may be useful for the future development of a reference assay. |
| first_indexed | 2024-12-21T08:57:58Z |
| format | Article |
| id | doaj.art-59a4ba03f30c45a08f0757d7428c5aff |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-21T08:57:58Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-59a4ba03f30c45a08f0757d7428c5aff2022-12-21T19:09:30ZengMDPI AGJournal of Fungi2309-608X2017-07-01333710.3390/jof3030037jof3030037Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatisAndrea Peano0Mario Pasquetti1Paolo Tizzani2Elisa Chiavassa3Jacques Guillot4Elizabeth Johnson5Dipartimento diScienze Veterinarie, Università di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDipartimento diScienze Veterinarie, Università di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDipartimento diScienze Veterinarie, Università di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDipartimento diScienze Veterinarie, Università di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyEcole nationale Vétérinaire d’Alfort, Dynamyc Research Group, EnvA, Université, Maisons-Alfort 94700, FrancePublic Health England Mycology Reference Laboratory, Kingsdown, Bristol BS2 8EL, UKReference methods for antifungal susceptibility testing of yeasts have been developed by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). These methods are intended to test the main pathogenic yeasts that cause invasive infections, namely Candida spp. and Cryptococcus neoformans, while testing other yeast species introduces several additional problems in standardization not addressed by these reference procedures. As a consequence, a number of procedures have been employed in the literature to test the antifungal susceptibility of Malassezia pachydermatis. This has resulted in conflicting results. The aim of the present study is to review the procedures and the technical parameters (growth media, inoculum preparation, temperature and length of incubation, method of reading) employed for susceptibility testing of M. pachydermatis, and when possible, to propose recommendations for or against their use. Such information may be useful for the future development of a reference assay.https://www.mdpi.com/2309-608X/3/3/37Malassezia pachydermatissusceptibility testingbroth microdilutiondisk diffusionminimum inhibitory concentration (MIC) |
| spellingShingle | Andrea Peano Mario Pasquetti Paolo Tizzani Elisa Chiavassa Jacques Guillot Elizabeth Johnson Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis Journal of Fungi Malassezia pachydermatis susceptibility testing broth microdilution disk diffusion minimum inhibitory concentration (MIC) |
| title | Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis |
| title_full | Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis |
| title_fullStr | Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis |
| title_full_unstemmed | Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis |
| title_short | Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis |
| title_sort | methodological issues in antifungal susceptibility testing of malassezia pachydermatis |
| topic | Malassezia pachydermatis susceptibility testing broth microdilution disk diffusion minimum inhibitory concentration (MIC) |
| url | https://www.mdpi.com/2309-608X/3/3/37 |
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