Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique
<p>Abstract</p> <p>Background</p> <p>Bovine tuberculosis (BTB), caused by <it>Mycobacterium bovis</it>, has remained a major source of concern to public health officials in Zambia. Previous investigations have used traditional epidemiological methods that ar...
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| Format: | Article |
| Language: | English |
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BMC
2009-07-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/144 |
| _version_ | 1818485127710244864 |
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| author | Tryland Morten Muma John Shamputa Isdore Rigouts Leen Munyeme Musso Skjerve Eystein Djønne Berit |
| author_facet | Tryland Morten Muma John Shamputa Isdore Rigouts Leen Munyeme Musso Skjerve Eystein Djønne Berit |
| author_sort | Tryland Morten |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bovine tuberculosis (BTB), caused by <it>Mycobacterium bovis</it>, has remained a major source of concern to public health officials in Zambia. Previous investigations have used traditional epidemiological methods that are unable to identify the causative agent and from which dynamics of disease dispersion is difficult to discern. The objective of this study was to isolate, characterize and determine the genetic diversity and relatedness of <it>M. bovis </it>from major cattle rearing districts in Zambia by spoligotyping. A total of 695 carcasses were examined and 98 tissues had gross post-mortem lesions compatible with BTB.</p> <p>Results</p> <p>Forty-two out of the ninety-eight suspected tissues examined had culture properties characteristic of mycobacteria from which 31 isolates yielded interpretable spoligotypes. This technique showed good discriminatory power (HGDI = 0.98), revealing 10 different spoligotype patterns. Twenty-seven isolates belonged to one cluster with more than 95% similarity and inside the cluster, one predominant spoligotype was found in 20 (64.5%) of the isolates tested. The highest number of spoligotypes was observed among samples from Namwala district. Spoligotypes from 26 (83.9%) of the isolates belonged to five spoligotypes that have been reported before while the remaining 5 (16.1%) isolates had unique spoligotypes that are being reported for the first time; these have been assigned numbers SB1763 to SB1767. Five of the 6 districts had the predominant spoligotype (SB0120).</p> <p>Conclusion</p> <p>The study has described the dispersion patterns of <it>M. bovis </it>in Zambian cattle for the first time and has identified 5 spoligotype patterns specific to Zambia. The observation of an overlap in the spoligotype pattern SB0120 in 5 of the 6 districts suggests the probability of sharing a common source of infection.</p> |
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| format | Article |
| id | doaj.art-59a7180f6f7f4008bcd0e1fe16ecd919 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T16:04:21Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-59a7180f6f7f4008bcd0e1fe16ecd9192022-12-22T01:42:17ZengBMCBMC Microbiology1471-21802009-07-019114410.1186/1471-2180-9-144Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing techniqueTryland MortenMuma JohnShamputa IsdoreRigouts LeenMunyeme MussoSkjerve EysteinDjønne Berit<p>Abstract</p> <p>Background</p> <p>Bovine tuberculosis (BTB), caused by <it>Mycobacterium bovis</it>, has remained a major source of concern to public health officials in Zambia. Previous investigations have used traditional epidemiological methods that are unable to identify the causative agent and from which dynamics of disease dispersion is difficult to discern. The objective of this study was to isolate, characterize and determine the genetic diversity and relatedness of <it>M. bovis </it>from major cattle rearing districts in Zambia by spoligotyping. A total of 695 carcasses were examined and 98 tissues had gross post-mortem lesions compatible with BTB.</p> <p>Results</p> <p>Forty-two out of the ninety-eight suspected tissues examined had culture properties characteristic of mycobacteria from which 31 isolates yielded interpretable spoligotypes. This technique showed good discriminatory power (HGDI = 0.98), revealing 10 different spoligotype patterns. Twenty-seven isolates belonged to one cluster with more than 95% similarity and inside the cluster, one predominant spoligotype was found in 20 (64.5%) of the isolates tested. The highest number of spoligotypes was observed among samples from Namwala district. Spoligotypes from 26 (83.9%) of the isolates belonged to five spoligotypes that have been reported before while the remaining 5 (16.1%) isolates had unique spoligotypes that are being reported for the first time; these have been assigned numbers SB1763 to SB1767. Five of the 6 districts had the predominant spoligotype (SB0120).</p> <p>Conclusion</p> <p>The study has described the dispersion patterns of <it>M. bovis </it>in Zambian cattle for the first time and has identified 5 spoligotype patterns specific to Zambia. The observation of an overlap in the spoligotype pattern SB0120 in 5 of the 6 districts suggests the probability of sharing a common source of infection.</p>http://www.biomedcentral.com/1471-2180/9/144 |
| spellingShingle | Tryland Morten Muma John Shamputa Isdore Rigouts Leen Munyeme Musso Skjerve Eystein Djønne Berit Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique BMC Microbiology |
| title | Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique |
| title_full | Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique |
| title_fullStr | Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique |
| title_full_unstemmed | Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique |
| title_short | Isolation and characterization of <it>Mycobacterium bovis </it>strains from indigenous Zambian cattle using Spacer oligonucleotide typing technique |
| title_sort | isolation and characterization of it mycobacterium bovis it strains from indigenous zambian cattle using spacer oligonucleotide typing technique |
| url | http://www.biomedcentral.com/1471-2180/9/144 |
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