Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation
Background: In patients with hepatocellular carcinoma (HCC), a complete clearance of circulating tumor cells (CTCs) early after liver transplantation (LT) or surgical resection (LR) could prevent tumor recurrence. Methods: prospective pilot study including patients with HCC who underwent LR or LT fr...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/10/2476 |
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| author | Víctor Amado Sandra González-Rubio Javier Zamora Rafael Alejandre María Lola Espejo-Cruz Clara Linares Marina Sánchez-Frías Gema García-Jurado José Luis Montero Rubén Ciria Manuel Rodríguez-Perálvarez Gustavo Ferrín Manuel De la Mata |
| author_facet | Víctor Amado Sandra González-Rubio Javier Zamora Rafael Alejandre María Lola Espejo-Cruz Clara Linares Marina Sánchez-Frías Gema García-Jurado José Luis Montero Rubén Ciria Manuel Rodríguez-Perálvarez Gustavo Ferrín Manuel De la Mata |
| author_sort | Víctor Amado |
| collection | DOAJ |
| description | Background: In patients with hepatocellular carcinoma (HCC), a complete clearance of circulating tumor cells (CTCs) early after liver transplantation (LT) or surgical resection (LR) could prevent tumor recurrence. Methods: prospective pilot study including patients with HCC who underwent LR or LT from September 2017 to May 2020. Enumeration of CTCs was performed in peripheral blood samples (7 mL) using the Isoflux<sup>®</sup> system (Fluxion Biosciences) immediately before surgery, at post-operative day 5 and at day 30. A clinically relevant number of CTCs was defined as >30 CTCs/sample. Results: 41 HCC patients were included (mean age 58.7 ± 6.3; 82.9% male). LR was performed in 10 patients (24.4%) and 31 patients (75.6%) underwent LT. The main etiology of liver disease was chronic hepatitis C (31.7%). Patients undergoing LR and LT were similar in terms of preoperative CTC count (<i>p</i> = 0.99), but clearance of CTCs within the first month was more pronounced in the LT group. Clusters of CTCs at baseline were associated with incomplete clearance of CTCs at day 30 (54.2% vs. 11.8%, <i>p</i> = 0.005), which in turn impacted negatively on survival (<i>p</i> = 0.038). Conclusion: Incomplete clearance of CTCs after surgery could be a surrogate marker of HCC aggressiveness. |
| first_indexed | 2024-03-10T11:16:06Z |
| format | Article |
| id | doaj.art-59afbde8af894feb83a30467de170e37 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T11:16:06Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-59afbde8af894feb83a30467de170e372023-11-21T20:26:04ZengMDPI AGCancers2072-66942021-05-011310247610.3390/cancers13102476Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver TransplantationVíctor Amado0Sandra González-Rubio1Javier Zamora2Rafael Alejandre3María Lola Espejo-Cruz4Clara Linares5Marina Sánchez-Frías6Gema García-Jurado7José Luis Montero8Rubén Ciria9Manuel Rodríguez-Perálvarez10Gustavo Ferrín11Manuel De la Mata12Department of Hepatology and Liver Transplantation, Reina Sofía University Hospital, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, 14004 Córdoba, SpainDepartment of Hepatology and Liver Transplantation, Reina Sofía University Hospital, 14004 Córdoba, SpainDepartment of Hepatology and Liver Transplantation, Reina Sofía University Hospital, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, 14004 Córdoba, SpainPathology Department, Reina Sofía University Hospital, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, 14004 Córdoba, SpainDepartment of Hepatology and Liver Transplantation, Reina Sofía University Hospital, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, 14004 Córdoba, SpainDepartment of Hepatology and Liver Transplantation, Reina Sofía University Hospital, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, 14004 Córdoba, SpainDepartment of Hepatology and Liver Transplantation, Reina Sofía University Hospital, 14004 Córdoba, SpainBackground: In patients with hepatocellular carcinoma (HCC), a complete clearance of circulating tumor cells (CTCs) early after liver transplantation (LT) or surgical resection (LR) could prevent tumor recurrence. Methods: prospective pilot study including patients with HCC who underwent LR or LT from September 2017 to May 2020. Enumeration of CTCs was performed in peripheral blood samples (7 mL) using the Isoflux<sup>®</sup> system (Fluxion Biosciences) immediately before surgery, at post-operative day 5 and at day 30. A clinically relevant number of CTCs was defined as >30 CTCs/sample. Results: 41 HCC patients were included (mean age 58.7 ± 6.3; 82.9% male). LR was performed in 10 patients (24.4%) and 31 patients (75.6%) underwent LT. The main etiology of liver disease was chronic hepatitis C (31.7%). Patients undergoing LR and LT were similar in terms of preoperative CTC count (<i>p</i> = 0.99), but clearance of CTCs within the first month was more pronounced in the LT group. Clusters of CTCs at baseline were associated with incomplete clearance of CTCs at day 30 (54.2% vs. 11.8%, <i>p</i> = 0.005), which in turn impacted negatively on survival (<i>p</i> = 0.038). Conclusion: Incomplete clearance of CTCs after surgery could be a surrogate marker of HCC aggressiveness.https://www.mdpi.com/2072-6694/13/10/2476hepatocellular carcinomaliver transplantationliver cancerliquid biopsycirculating tumor cell |
| spellingShingle | Víctor Amado Sandra González-Rubio Javier Zamora Rafael Alejandre María Lola Espejo-Cruz Clara Linares Marina Sánchez-Frías Gema García-Jurado José Luis Montero Rubén Ciria Manuel Rodríguez-Perálvarez Gustavo Ferrín Manuel De la Mata Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation Cancers hepatocellular carcinoma liver transplantation liver cancer liquid biopsy circulating tumor cell |
| title | Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation |
| title_full | Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation |
| title_fullStr | Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation |
| title_full_unstemmed | Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation |
| title_short | Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation |
| title_sort | clearance of circulating tumor cells in patients with hepatocellular carcinoma undergoing surgical resection or liver transplantation |
| topic | hepatocellular carcinoma liver transplantation liver cancer liquid biopsy circulating tumor cell |
| url | https://www.mdpi.com/2072-6694/13/10/2476 |
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