Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
Abstract Background Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were ga...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-021-02919-5 |
| _version_ | 1819065506465841152 |
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| author | Julia Stadler Julia Ade Walter Hermanns Mathias Ritzmann Sarah Wentzel Katharina Hoelzle Ludwig E. Hoelzle |
| author_facet | Julia Stadler Julia Ade Walter Hermanns Mathias Ritzmann Sarah Wentzel Katharina Hoelzle Ludwig E. Hoelzle |
| author_sort | Julia Stadler |
| collection | DOAJ |
| description | Abstract Background Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were gained by the use of new molecular techniques. However, details on M. suis induced lesions as well as the distribution of M. suis in different organs are still lacking. Therefore, seven splenectomised pigs were experimentally infected and clinical and laboratory investigations as well as a detailed histopathological examination were performed. Detection and quantification of M. suis DNA in blood and various tissue samples was done using a quantitative real-time PCR. Results During the course of experimental infection, periodically occurring signs of infectious anaemia of pigs including severe icteroanaemia, fever, apathy and anorexia were observed. In addition, dermatological manifestations such as haemorrhagic diathesis presenting as petechiae occurred. The most important haematological alterations were normochromic, normocytic anaemia, hypoglycaemia as well as increased bilirubin and urea concentrations. Necropsy revealed predominant evidence of haemolysis with consecutive anaemia, as well as disseminated intravascular coagulation. M. suis was found in all investigated tissues with the highest copy numbers found in the kidneys. In Giemsa stained sections M. suis was only detected red blood cell (RBC)-associated. Conclusion In the present study, no RBC independent sequestration of M. suis was detected in organs of experimentally infected pigs. Pathological findings are most likely resulting from haemolysis, consecutive anaemia as well as from disseminated intravascular coagulation and subsequent organ impairments. |
| first_indexed | 2024-12-21T15:47:33Z |
| format | Article |
| id | doaj.art-59b215732f1849ee99e1dbd1a2155f7c |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T15:47:33Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-59b215732f1849ee99e1dbd1a2155f7c2022-12-21T18:58:19ZengBMCBMC Veterinary Research1746-61482021-06-0117111010.1186/s12917-021-02919-5Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigsJulia Stadler0Julia Ade1Walter Hermanns2Mathias Ritzmann3Sarah Wentzel4Katharina Hoelzle5Ludwig E. Hoelzle6Clinic for Swine, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University MunichInstitute of Animal Science, University of HohenheimInstitute of Veterinary Pathology, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University MunichClinic for Swine, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University MunichClinic for Swine, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University MunichInstitute of Animal Science, University of HohenheimInstitute of Animal Science, University of HohenheimAbstract Background Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were gained by the use of new molecular techniques. However, details on M. suis induced lesions as well as the distribution of M. suis in different organs are still lacking. Therefore, seven splenectomised pigs were experimentally infected and clinical and laboratory investigations as well as a detailed histopathological examination were performed. Detection and quantification of M. suis DNA in blood and various tissue samples was done using a quantitative real-time PCR. Results During the course of experimental infection, periodically occurring signs of infectious anaemia of pigs including severe icteroanaemia, fever, apathy and anorexia were observed. In addition, dermatological manifestations such as haemorrhagic diathesis presenting as petechiae occurred. The most important haematological alterations were normochromic, normocytic anaemia, hypoglycaemia as well as increased bilirubin and urea concentrations. Necropsy revealed predominant evidence of haemolysis with consecutive anaemia, as well as disseminated intravascular coagulation. M. suis was found in all investigated tissues with the highest copy numbers found in the kidneys. In Giemsa stained sections M. suis was only detected red blood cell (RBC)-associated. Conclusion In the present study, no RBC independent sequestration of M. suis was detected in organs of experimentally infected pigs. Pathological findings are most likely resulting from haemolysis, consecutive anaemia as well as from disseminated intravascular coagulation and subsequent organ impairments.https://doi.org/10.1186/s12917-021-02919-5Haemotrophic mycoplasmasMycoplasma suisHistopathological findingsTissue sequestration |
| spellingShingle | Julia Stadler Julia Ade Walter Hermanns Mathias Ritzmann Sarah Wentzel Katharina Hoelzle Ludwig E. Hoelzle Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs BMC Veterinary Research Haemotrophic mycoplasmas Mycoplasma suis Histopathological findings Tissue sequestration |
| title | Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs |
| title_full | Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs |
| title_fullStr | Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs |
| title_full_unstemmed | Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs |
| title_short | Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs |
| title_sort | clinical haematological and pathomorphological findings in mycoplasma suis infected pigs |
| topic | Haemotrophic mycoplasmas Mycoplasma suis Histopathological findings Tissue sequestration |
| url | https://doi.org/10.1186/s12917-021-02919-5 |
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