Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment
Isoflurane is commonly used in pediatric population, but its mechanism of action in cognition is unclear. Aquaporin 4 (AQP4) regulates water content in blood, brain, and cerebrospinal fluid. Various studies have provided evidence for the role of AQP4 in synaptic plasticity and neurocognition. In th...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2019-08-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4116 |
| _version_ | 1797260882863980544 |
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| author | Serdar Demirgan Onat Akyol Zeynep Temel Aslıhan Şengelen Murat Pekmez Recep Demirgan Mehmet Salih Sevdi Kerem Erkalp Ayşin Selcan |
| author_facet | Serdar Demirgan Onat Akyol Zeynep Temel Aslıhan Şengelen Murat Pekmez Recep Demirgan Mehmet Salih Sevdi Kerem Erkalp Ayşin Selcan |
| author_sort | Serdar Demirgan |
| collection | DOAJ |
| description |
Isoflurane is commonly used in pediatric population, but its mechanism of action in cognition is unclear. Aquaporin 4 (AQP4) regulates water content in blood, brain, and cerebrospinal fluid. Various studies have provided evidence for the role of AQP4 in synaptic plasticity and neurocognition. In this study, we aimed to determine whether a prolonged exposure to isoflurane in infant rats is associated with cognition and what effect this exposure has on AQP4 expression. Ten-day-old [postnatal day (P) 10] Wistar albino rats were randomly allocated to isoflurane group (n = 32; 1.5% isoflurane in 50% oxygen for 6 hours) or control group (n = 32; only 50% oxygen for 6 hours). Acute (P11) and long-term (P33) effects of 6-hour anesthetic isoflurane exposure on AQP4 expression were analyzed in whole brains of P11 and P33 rats by RT-qPCR and Western blot. Spatial learning and memory were assessed on P28 to P33 days by Morris Water Maze (MWM) test. The analysis revealed that isoflurane increased acutely both mRNA (~4.5 fold) and protein (~90%) levels of AQP4 in P11 rats compared with control group. The increasing levels of AQP4 in P11 were not observed in P33 rats. Also, no statistically significant change between isoflurane and control groups was observed in the latency to find the platform during MWM training and probe trial. Our results indicate that a single exposure to isoflurane anesthesia does not influence cognition in infant rats. In this case, acutely increased AQP4 after isoflurane anesthesia may have a protective role in neurocognition.
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| first_indexed | 2024-04-24T23:32:23Z |
| format | Article |
| id | doaj.art-59b49b8a3a0a40dc92f99cba52dbcb14 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:23Z |
| publishDate | 2019-08-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-59b49b8a3a0a40dc92f99cba52dbcb142024-03-15T14:30:13ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2019-08-0119310.17305/bjbms.2019.4116Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairmentSerdar Demirgan0https://orcid.org/0000-0001-8129-5004Onat Akyol1https://orcid.org/0000-0003-1316-7160Zeynep Temel2https://orcid.org/0000-0001-8563-5635Aslıhan Şengelen3https://orcid.org/0000-0003-2512-319XMurat Pekmez4https://orcid.org/0000-0002-6150-8372Recep Demirgan5https://orcid.org/0000-0002-2653-4815Mehmet Salih Sevdi6Kerem Erkalp7Ayşin Selcan8T.C. Health Ministry, Health Sciences University, Bagcilar Training and Research Hospital, Anesthesiology and Reanimation Clinic; Department of Molecular Biology and Genetics, Institute of Graduate Studies in Sciences, Istanbul University, Istanbul, TurkeyT.C. Health Ministry, Health Sciences University, Bagcilar Training and Research Hospital, Anesthesiology and Reanimation Clinic, Istanbul, TurkeyDepartment of Neuroscience Institute of Health Sciences, Istanbul Medipol University, Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Institute of Graduate Studies in Sciences, Istanbul University, Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Institute of Graduate Studies in Sciences, Istanbul University, Istanbul, TurkeyT.C. Health Ministry, Health Sciences University, Bagcilar Training and Research Hospital, Anesthesiology and Reanimation Clinic, Istanbul, TurkeyT.C. Health Ministry, Health Sciences University, Bagcilar Training and Research Hospital, Anesthesiology and Reanimation Clinic, Istanbul, TurkeyT.C. Health Ministry, Health Sciences University, Bagcilar Training and Research Hospital, Anesthesiology and Reanimation Clinic, Istanbul, Turkey Isoflurane is commonly used in pediatric population, but its mechanism of action in cognition is unclear. Aquaporin 4 (AQP4) regulates water content in blood, brain, and cerebrospinal fluid. Various studies have provided evidence for the role of AQP4 in synaptic plasticity and neurocognition. In this study, we aimed to determine whether a prolonged exposure to isoflurane in infant rats is associated with cognition and what effect this exposure has on AQP4 expression. Ten-day-old [postnatal day (P) 10] Wistar albino rats were randomly allocated to isoflurane group (n = 32; 1.5% isoflurane in 50% oxygen for 6 hours) or control group (n = 32; only 50% oxygen for 6 hours). Acute (P11) and long-term (P33) effects of 6-hour anesthetic isoflurane exposure on AQP4 expression were analyzed in whole brains of P11 and P33 rats by RT-qPCR and Western blot. Spatial learning and memory were assessed on P28 to P33 days by Morris Water Maze (MWM) test. The analysis revealed that isoflurane increased acutely both mRNA (~4.5 fold) and protein (~90%) levels of AQP4 in P11 rats compared with control group. The increasing levels of AQP4 in P11 were not observed in P33 rats. Also, no statistically significant change between isoflurane and control groups was observed in the latency to find the platform during MWM training and probe trial. Our results indicate that a single exposure to isoflurane anesthesia does not influence cognition in infant rats. In this case, acutely increased AQP4 after isoflurane anesthesia may have a protective role in neurocognition. https://www.bjbms.org/ojs/index.php/bjbms/article/view/4116Anesthesiaaquaporin 4 (AQP4)neurocognitioninfant ratisofluranepediatric anesthesia |
| spellingShingle | Serdar Demirgan Onat Akyol Zeynep Temel Aslıhan Şengelen Murat Pekmez Recep Demirgan Mehmet Salih Sevdi Kerem Erkalp Ayşin Selcan Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment Biomolecules & Biomedicine Anesthesia aquaporin 4 (AQP4) neurocognition infant rat isoflurane pediatric anesthesia |
| title | Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment |
| title_full | Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment |
| title_fullStr | Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment |
| title_full_unstemmed | Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment |
| title_short | Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment |
| title_sort | isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment |
| topic | Anesthesia aquaporin 4 (AQP4) neurocognition infant rat isoflurane pediatric anesthesia |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4116 |
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