The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i>
Sublethal doses of insecticides have many impacts on pest control and agroecosystems. Insects that survive a sublethal dose of insecticide could adapt their physiological and behavioral functions and resist this environmental stress, which contributes to the challenge of pest management. In this stu...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9388 |
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| author | Xueting Qu Sijia Wang Guangze Lin Mingshan Li Jie Shen Dan Wang |
| author_facet | Xueting Qu Sijia Wang Guangze Lin Mingshan Li Jie Shen Dan Wang |
| author_sort | Xueting Qu |
| collection | DOAJ |
| description | Sublethal doses of insecticides have many impacts on pest control and agroecosystems. Insects that survive a sublethal dose of insecticide could adapt their physiological and behavioral functions and resist this environmental stress, which contributes to the challenge of pest management. In this study, the sublethal effects of thiamethoxam on gene expression were measured through RNA sequencing in the melon aphid <i>Aphis gossypii</i>. Genes regulating energy production were downregulated, while genes related to neural function were upregulated. To further address the function of genes related to neurotransmission, RNA interference (RNAi) was implemented by transdermal delivery of dsRNA targeting <i>synapsin</i> (<i>syn</i>), a gene regulating presynaptic vesicle clustering. The gene expression of <i>synapsin</i> was knocked down and the mortality of aphids was increased significantly over the duration of the assay. Co-delivery of syn-dsRNA and thiamethoxam reversed the upregulation of <i>synapsin</i> caused by low-dose thiamethoxam and resulted in lethality to melon aphids, suggesting that the decreased presynaptic function may contribute to this synergistic lethal effect. In addition, the nanocarrier star polycation, which could bind both dsRNA and thiamethoxam, greatly improved the efficacy of lethality. These results increase our knowledge of the gene regulation induced by sublethal exposure to neonicotinoids and indicated that <i>synapsin</i> could be a potential RNAi target for resistance management of the melon aphid. |
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| issn | 1661-6596 1422-0067 |
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| spelling | doaj.art-59b6f7ff0d3e4f5ab1a754dd6468f6c92023-11-30T21:36:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316938810.3390/ijms23169388The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i>Xueting Qu0Sijia Wang1Guangze Lin2Mingshan Li3Jie Shen4Dan Wang5Department of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaDepartment of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaDepartment of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaDepartment of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaDepartment of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaDepartment of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaSublethal doses of insecticides have many impacts on pest control and agroecosystems. Insects that survive a sublethal dose of insecticide could adapt their physiological and behavioral functions and resist this environmental stress, which contributes to the challenge of pest management. In this study, the sublethal effects of thiamethoxam on gene expression were measured through RNA sequencing in the melon aphid <i>Aphis gossypii</i>. Genes regulating energy production were downregulated, while genes related to neural function were upregulated. To further address the function of genes related to neurotransmission, RNA interference (RNAi) was implemented by transdermal delivery of dsRNA targeting <i>synapsin</i> (<i>syn</i>), a gene regulating presynaptic vesicle clustering. The gene expression of <i>synapsin</i> was knocked down and the mortality of aphids was increased significantly over the duration of the assay. Co-delivery of syn-dsRNA and thiamethoxam reversed the upregulation of <i>synapsin</i> caused by low-dose thiamethoxam and resulted in lethality to melon aphids, suggesting that the decreased presynaptic function may contribute to this synergistic lethal effect. In addition, the nanocarrier star polycation, which could bind both dsRNA and thiamethoxam, greatly improved the efficacy of lethality. These results increase our knowledge of the gene regulation induced by sublethal exposure to neonicotinoids and indicated that <i>synapsin</i> could be a potential RNAi target for resistance management of the melon aphid.https://www.mdpi.com/1422-0067/23/16/9388RNA interferenceneonicotinoid<i>synapsin</i>pest controlnanocarrier |
| spellingShingle | Xueting Qu Sijia Wang Guangze Lin Mingshan Li Jie Shen Dan Wang The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i> International Journal of Molecular Sciences RNA interference neonicotinoid <i>synapsin</i> pest control nanocarrier |
| title | The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i> |
| title_full | The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i> |
| title_fullStr | The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i> |
| title_full_unstemmed | The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i> |
| title_short | The Synergistic Effect of Thiamethoxam and Synapsin dsRNA Targets Neurotransmission to Induce Mortality in <i>Aphis gossypii</i> |
| title_sort | synergistic effect of thiamethoxam and synapsin dsrna targets neurotransmission to induce mortality in i aphis gossypii i |
| topic | RNA interference neonicotinoid <i>synapsin</i> pest control nanocarrier |
| url | https://www.mdpi.com/1422-0067/23/16/9388 |
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