Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms
Chronic wasting disease (CWD) is a prion disease of North American cervids. The transmission of CWD to endangered cervid species is of concern for captive breeding programs. Trans-species transmission could occur via direct contact with infected wild deer, or via prion contaminated fomites. Variatio...
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Frontiers Media S.A.
2022-10-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcosc.2022.1007100/full |
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| author | Tolulope I.N. Perrin-Stowe Tolulope I.N. Perrin-Stowe Yasuko Ishida Dolores M. Reed Emily E. Terrill Oliver A. Ryder Oliver A. Ryder Jan E. Novakofski Jan E. Novakofski Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Budhan S. Pukazhenthi Alfred L. Roca Alfred L. Roca Alfred L. Roca Alfred L. Roca Alfred L. Roca |
| author_facet | Tolulope I.N. Perrin-Stowe Tolulope I.N. Perrin-Stowe Yasuko Ishida Dolores M. Reed Emily E. Terrill Oliver A. Ryder Oliver A. Ryder Jan E. Novakofski Jan E. Novakofski Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Budhan S. Pukazhenthi Alfred L. Roca Alfred L. Roca Alfred L. Roca Alfred L. Roca Alfred L. Roca |
| author_sort | Tolulope I.N. Perrin-Stowe |
| collection | DOAJ |
| description | Chronic wasting disease (CWD) is a prion disease of North American cervids. The transmission of CWD to endangered cervid species is of concern for captive breeding programs. Trans-species transmission could occur via direct contact with infected wild deer, or via prion contaminated fomites. Variation in the prion protein gene, PRNP, is associated with differences in CWD susceptibility among cervids. We therefore sequenced PRNP in 36 endangered Eld’s deer (Rucervus eldii thamin), detecting five synonymous and two non-synonymous SNPs. Three haplotypes were inferred, suggesting that genetic management in captive breeding programs has been effective at maintaining PRNP diversity. The haplotypes encoded two PrP protein variants. The more common Eld’s deer PrP variant encodes methionine at codon 208 and glutamine at codon 226. Because this protein variant is identical to a common PrP variant in white-tailed deer and mule deer and is especially common in white-tailed deer positive for CWD, we recommend reducing the frequency of this variant in the breeding stock, while implementing strict management practices to avoid exposure to wild North American cervids. The frequency of the other PrP variant, which differs from variants present in these North American cervids, was low. It has the potential to reduce susceptibility to CWD and thus could be increased in frequency. While PRNP haplotype frequencies should be shifted, genetic diversity should be maintained. Ultimately protein diversity may be protective should CWD infect the species, and trans-species polymorphisms are suggestive of past balancing selection and a potential fitness advantage for PRNP diversity. |
| first_indexed | 2024-04-11T10:05:05Z |
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| language | English |
| last_indexed | 2024-04-11T10:05:05Z |
| publishDate | 2022-10-01 |
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| series | Frontiers in Conservation Science |
| spelling | doaj.art-59b74ee9a3124d3b82a0a1e9673ed6b72022-12-22T04:30:15ZengFrontiers Media S.A.Frontiers in Conservation Science2673-611X2022-10-01310.3389/fcosc.2022.10071001007100Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphismsTolulope I.N. Perrin-Stowe0Tolulope I.N. Perrin-Stowe1Yasuko Ishida2Dolores M. Reed3Emily E. Terrill4Oliver A. Ryder5Oliver A. Ryder6Jan E. Novakofski7Jan E. Novakofski8Nohra E. Mateus-Pinilla9Nohra E. Mateus-Pinilla10Nohra E. Mateus-Pinilla11Nohra E. Mateus-Pinilla12Budhan S. Pukazhenthi13Alfred L. Roca14Alfred L. Roca15Alfred L. Roca16Alfred L. Roca17Alfred L. Roca18Program in Ecology, Evolution, and Conservation Biology, School of Integrative Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesSchool of Integrative Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesDepartment of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesCenter for Species Survival, Smithsonian’s National Zoo and Conservation Biology Institute, Front Royal, VA, United StatesSchool of Integrative Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesSan Diego Zoo Institute for Conservation Research, Escondido, CA, United StatesDepartment of Evolution, Behavior, and Ecology, Division of Biology, University of California San Diego, La Jolla, CA, United StatesDepartment of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesIllinois Natural History Survey-Prairie Research Institute, Champaign, IL, United StatesDepartment of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesIllinois Natural History Survey-Prairie Research Institute, Champaign, IL, United StatesDepartment of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesDepartment of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesCenter for Species Survival, Smithsonian’s National Zoo and Conservation Biology Institute, Front Royal, VA, United StatesProgram in Ecology, Evolution, and Conservation Biology, School of Integrative Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesDepartment of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesIllinois Natural History Survey-Prairie Research Institute, Champaign, IL, United StatesDepartment of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United States0Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesChronic wasting disease (CWD) is a prion disease of North American cervids. The transmission of CWD to endangered cervid species is of concern for captive breeding programs. Trans-species transmission could occur via direct contact with infected wild deer, or via prion contaminated fomites. Variation in the prion protein gene, PRNP, is associated with differences in CWD susceptibility among cervids. We therefore sequenced PRNP in 36 endangered Eld’s deer (Rucervus eldii thamin), detecting five synonymous and two non-synonymous SNPs. Three haplotypes were inferred, suggesting that genetic management in captive breeding programs has been effective at maintaining PRNP diversity. The haplotypes encoded two PrP protein variants. The more common Eld’s deer PrP variant encodes methionine at codon 208 and glutamine at codon 226. Because this protein variant is identical to a common PrP variant in white-tailed deer and mule deer and is especially common in white-tailed deer positive for CWD, we recommend reducing the frequency of this variant in the breeding stock, while implementing strict management practices to avoid exposure to wild North American cervids. The frequency of the other PrP variant, which differs from variants present in these North American cervids, was low. It has the potential to reduce susceptibility to CWD and thus could be increased in frequency. While PRNP haplotype frequencies should be shifted, genetic diversity should be maintained. Ultimately protein diversity may be protective should CWD infect the species, and trans-species polymorphisms are suggestive of past balancing selection and a potential fitness advantage for PRNP diversity.https://www.frontiersin.org/articles/10.3389/fcosc.2022.1007100/fullbrow-antlered deercervidsprionthamintransmissible spongiform encephalopathy |
| spellingShingle | Tolulope I.N. Perrin-Stowe Tolulope I.N. Perrin-Stowe Yasuko Ishida Dolores M. Reed Emily E. Terrill Oliver A. Ryder Oliver A. Ryder Jan E. Novakofski Jan E. Novakofski Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Nohra E. Mateus-Pinilla Budhan S. Pukazhenthi Alfred L. Roca Alfred L. Roca Alfred L. Roca Alfred L. Roca Alfred L. Roca Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms Frontiers in Conservation Science brow-antlered deer cervids prion thamin transmissible spongiform encephalopathy |
| title | Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms |
| title_full | Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms |
| title_fullStr | Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms |
| title_full_unstemmed | Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms |
| title_short | Extrapolating the susceptibility of Eld’s deer (Rucervus eldii thamin) to chronic wasting disease from prion protein gene (PRNP) polymorphisms |
| title_sort | extrapolating the susceptibility of eld s deer rucervus eldii thamin to chronic wasting disease from prion protein gene prnp polymorphisms |
| topic | brow-antlered deer cervids prion thamin transmissible spongiform encephalopathy |
| url | https://www.frontiersin.org/articles/10.3389/fcosc.2022.1007100/full |
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