A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (<A HREF="http://biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html</A>), working with the Arizo...

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Bibliographic Details
Main Authors: Coen M Adema, Mei-Zhong Luo, Ben Hanelt, Lynn A Hertel, Jennifer J Marshall, Si-Ming Zhang, Randall J DeJong, Hye-Ran Kim, David Kudrna, Rod A Wing, Cari Soderlund, Matty Knight, Fred A Lewis, Roberta Lima Caldeira, Liana K Jannotti-Passos, Omar dos Santos Carvalho, Eric S Loker
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2006-10-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
genomics
gene discovery
fingerprinting
schistosomiasis
medical malacology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900027
Description
Summary:To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (<A HREF="http://biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html</A>), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.
ISSN:0074-0276
1678-8060

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