A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (<A HREF="http://biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html</A>), working with the Arizo...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2006-10-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900027 |
| _version_ | 1797758436302127104 |
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| author | Coen M Adema Mei-Zhong Luo Ben Hanelt Lynn A Hertel Jennifer J Marshall Si-Ming Zhang Randall J DeJong Hye-Ran Kim David Kudrna Rod A Wing Cari Soderlund Matty Knight Fred A Lewis Roberta Lima Caldeira Liana K Jannotti-Passos Omar dos Santos Carvalho Eric S Loker |
| author_facet | Coen M Adema Mei-Zhong Luo Ben Hanelt Lynn A Hertel Jennifer J Marshall Si-Ming Zhang Randall J DeJong Hye-Ran Kim David Kudrna Rod A Wing Cari Soderlund Matty Knight Fred A Lewis Roberta Lima Caldeira Liana K Jannotti-Passos Omar dos Santos Carvalho Eric S Loker |
| author_sort | Coen M Adema |
| collection | DOAJ |
| description | To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (<A HREF="http://biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html</A>), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI. |
| first_indexed | 2024-03-12T18:29:57Z |
| format | Article |
| id | doaj.art-59b9345bb707435cbdaa8bc6ca13390c |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:29:57Z |
| publishDate | 2006-10-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-59b9345bb707435cbdaa8bc6ca13390c2023-08-02T08:19:26ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602006-10-0110116717710.1590/S0074-02762006000900027A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoniCoen M AdemaMei-Zhong LuoBen HaneltLynn A HertelJennifer J MarshallSi-Ming ZhangRandall J DeJongHye-Ran KimDavid KudrnaRod A WingCari SoderlundMatty KnightFred A LewisRoberta Lima CaldeiraLiana K Jannotti-PassosOmar dos Santos CarvalhoEric S LokerTo provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (<A HREF="http://biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html</A>), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900027genomicsgene discoveryfingerprintingschistosomiasismedical malacology |
| spellingShingle | Coen M Adema Mei-Zhong Luo Ben Hanelt Lynn A Hertel Jennifer J Marshall Si-Ming Zhang Randall J DeJong Hye-Ran Kim David Kudrna Rod A Wing Cari Soderlund Matty Knight Fred A Lewis Roberta Lima Caldeira Liana K Jannotti-Passos Omar dos Santos Carvalho Eric S Loker A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni Memorias do Instituto Oswaldo Cruz genomics gene discovery fingerprinting schistosomiasis medical malacology |
| title | A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_full | A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_fullStr | A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_full_unstemmed | A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_short | A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_sort | bacterial artificial chromosome library for biomphalaria glabrata intermediate snail host of schistosoma mansoni |
| topic | genomics gene discovery fingerprinting schistosomiasis medical malacology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900027 |
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