Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories

In this work, multivariate detection limits (MDL) estimator was obtained based on the micro-electro-mechanical systems–near infrared (MEMS–NIR) technology coupled with two sampling accessories to assess the detection capability of four quality parameters (glycyrrhizic acid, liquiritin, liquiritigeni...

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Main Authors: Zhisheng Wu, Xinyuan Shi, Na Zhao, Yanling Pei, Manfei Xu, Luwei Zhou, Yang Li, Yanjiang Qiao
Format: Article
Language:English
Published: World Scientific Publishing 2015-09-01
Series:Journal of Innovative Optical Health Sciences
Subjects:
Online Access:http://www.worldscientific.com/doi/pdf/10.1142/S1793545815500091
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author Zhisheng Wu
Xinyuan Shi
Na Zhao
Yanling Pei
Manfei Xu
Luwei Zhou
Yang Li
Yanjiang Qiao
author_facet Zhisheng Wu
Xinyuan Shi
Na Zhao
Yanling Pei
Manfei Xu
Luwei Zhou
Yang Li
Yanjiang Qiao
author_sort Zhisheng Wu
collection DOAJ
description In this work, multivariate detection limits (MDL) estimator was obtained based on the micro-electro-mechanical systems–near infrared (MEMS–NIR) technology coupled with two sampling accessories to assess the detection capability of four quality parameters (glycyrrhizic acid, liquiritin, liquiritigenin and isoliquiritin) in licorice from different geographical regions. 112 licorice samples were divided into two parts (calibration set and prediction set) using Kennard–Stone (KS) method. Four quality parameters were measured using high-performance liquid chromatography (HPLC) method according to Chinese pharmacopoeia and previous studies. The MEMS–NIR spectra were acquired from fiber optic probe (FOP) and integrating sphere, then the partial least squares (PLS) model was obtained using the optimum processing method. Chemometrics indicators have been utilized to assess the PLS model performance. Model assessment using chemometrics indicators is based on relative mean prediction error of all concentration levels, which indicated relatively low sensitivity for low-content analytes (below 1000 parts per million (ppm)). Therefore, MDL estimator was introduced with alpha error and beta error based on good prediction characteristic of low concentration levels. The result suggested that MEMS–NIR technology coupled with fiber optic probe (FOP) and integrating sphere was able to detect minor analytes. The result further demonstrated that integrating sphere mode (i.e., MDL0.05,0.05, 0.22%) was more robust than FOP mode (i.e., MDL0.05,0.05, 0.48%). In conclusion, this research proposed that MDL method was helpful to determine the detection capabilities of low-content analytes using MEMS–NIR technology and successful to compare two sampling accessories.
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spelling doaj.art-59bcf865208e47018113bcd45a55b9382022-12-22T01:54:44ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052015-09-01851550009-11550009-1010.1142/S179354581550009110.1142/S1793545815500091Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessoriesZhisheng Wu0Xinyuan Shi1Na Zhao2Yanling Pei3Manfei Xu4Luwei Zhou5Yang Li6Yanjiang Qiao7Beijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaBeijing University of Chinese Medicine, P. R. China 100102, P. R. ChinaIn this work, multivariate detection limits (MDL) estimator was obtained based on the micro-electro-mechanical systems–near infrared (MEMS–NIR) technology coupled with two sampling accessories to assess the detection capability of four quality parameters (glycyrrhizic acid, liquiritin, liquiritigenin and isoliquiritin) in licorice from different geographical regions. 112 licorice samples were divided into two parts (calibration set and prediction set) using Kennard–Stone (KS) method. Four quality parameters were measured using high-performance liquid chromatography (HPLC) method according to Chinese pharmacopoeia and previous studies. The MEMS–NIR spectra were acquired from fiber optic probe (FOP) and integrating sphere, then the partial least squares (PLS) model was obtained using the optimum processing method. Chemometrics indicators have been utilized to assess the PLS model performance. Model assessment using chemometrics indicators is based on relative mean prediction error of all concentration levels, which indicated relatively low sensitivity for low-content analytes (below 1000 parts per million (ppm)). Therefore, MDL estimator was introduced with alpha error and beta error based on good prediction characteristic of low concentration levels. The result suggested that MEMS–NIR technology coupled with fiber optic probe (FOP) and integrating sphere was able to detect minor analytes. The result further demonstrated that integrating sphere mode (i.e., MDL0.05,0.05, 0.22%) was more robust than FOP mode (i.e., MDL0.05,0.05, 0.48%). In conclusion, this research proposed that MDL method was helpful to determine the detection capabilities of low-content analytes using MEMS–NIR technology and successful to compare two sampling accessories.http://www.worldscientific.com/doi/pdf/10.1142/S1793545815500091Near-infrared spectrometermultivariate detection limitssampling accessorieslicoricepartial least squares regression
spellingShingle Zhisheng Wu
Xinyuan Shi
Na Zhao
Yanling Pei
Manfei Xu
Luwei Zhou
Yang Li
Yanjiang Qiao
Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories
Journal of Innovative Optical Health Sciences
Near-infrared spectrometer
multivariate detection limits
sampling accessories
licorice
partial least squares regression
title Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories
title_full Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories
title_fullStr Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories
title_full_unstemmed Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories
title_short Estimation of multivariate detection limits of four quality parameters in licorice using MEMS–NIR spectrometry coupled with two sampling accessories
title_sort estimation of multivariate detection limits of four quality parameters in licorice using mems nir spectrometry coupled with two sampling accessories
topic Near-infrared spectrometer
multivariate detection limits
sampling accessories
licorice
partial least squares regression
url http://www.worldscientific.com/doi/pdf/10.1142/S1793545815500091
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