In Vivo Imaging with Genetically Encoded Redox Biosensors
Redox reactions are of high fundamental and practical interest since they are involved in both normal physiology and the pathogenesis of various diseases. However, this area of research has always been a relatively problematic field in the context of analytical approaches, mostly because of the unst...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/21/8164 |
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| author | Alexander I. Kostyuk Anastasiya S. Panova Aleksandra D. Kokova Daria A. Kotova Dmitry I. Maltsev Oleg V. Podgorny Vsevolod V. Belousov Dmitry S. Bilan |
| author_facet | Alexander I. Kostyuk Anastasiya S. Panova Aleksandra D. Kokova Daria A. Kotova Dmitry I. Maltsev Oleg V. Podgorny Vsevolod V. Belousov Dmitry S. Bilan |
| author_sort | Alexander I. Kostyuk |
| collection | DOAJ |
| description | Redox reactions are of high fundamental and practical interest since they are involved in both normal physiology and the pathogenesis of various diseases. However, this area of research has always been a relatively problematic field in the context of analytical approaches, mostly because of the unstable nature of the compounds that are measured. Genetically encoded sensors allow for the registration of highly reactive molecules in real-time mode and, therefore, they began a new era in redox biology. Their strongest points manifest most brightly in in vivo experiments and pave the way for the non-invasive investigation of biochemical pathways that proceed in organisms from different systematic groups. In the first part of the review, we briefly describe the redox sensors that were used in vivo as well as summarize the model systems to which they were applied. Next, we thoroughly discuss the biological results obtained in these studies in regard to animals, plants, as well as unicellular eukaryotes and prokaryotes. We hope that this work reflects the amazing power of this technology and can serve as a useful guide for biologists and chemists who work in the field of redox processes. |
| first_indexed | 2024-03-10T15:10:18Z |
| format | Article |
| id | doaj.art-59bf1bd092cb4f6189e23a7be7370679 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:10:18Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-59bf1bd092cb4f6189e23a7be73706792023-11-20T19:24:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012121816410.3390/ijms21218164In Vivo Imaging with Genetically Encoded Redox BiosensorsAlexander I. Kostyuk0Anastasiya S. Panova1Aleksandra D. Kokova2Daria A. Kotova3Dmitry I. Maltsev4Oleg V. Podgorny5Vsevolod V. Belousov6Dmitry S. Bilan7Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, RussiaRedox reactions are of high fundamental and practical interest since they are involved in both normal physiology and the pathogenesis of various diseases. However, this area of research has always been a relatively problematic field in the context of analytical approaches, mostly because of the unstable nature of the compounds that are measured. Genetically encoded sensors allow for the registration of highly reactive molecules in real-time mode and, therefore, they began a new era in redox biology. Their strongest points manifest most brightly in in vivo experiments and pave the way for the non-invasive investigation of biochemical pathways that proceed in organisms from different systematic groups. In the first part of the review, we briefly describe the redox sensors that were used in vivo as well as summarize the model systems to which they were applied. Next, we thoroughly discuss the biological results obtained in these studies in regard to animals, plants, as well as unicellular eukaryotes and prokaryotes. We hope that this work reflects the amazing power of this technology and can serve as a useful guide for biologists and chemists who work in the field of redox processes.https://www.mdpi.com/1422-0067/21/21/8164fluorescent proteinsgenetically encoded sensorsglutathione (GSH), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), in vivo imagingmycothiol (MSH), NADHNADPHreactive oxygen species (ROS) |
| spellingShingle | Alexander I. Kostyuk Anastasiya S. Panova Aleksandra D. Kokova Daria A. Kotova Dmitry I. Maltsev Oleg V. Podgorny Vsevolod V. Belousov Dmitry S. Bilan In Vivo Imaging with Genetically Encoded Redox Biosensors International Journal of Molecular Sciences fluorescent proteins genetically encoded sensors glutathione (GSH), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), in vivo imaging mycothiol (MSH), NADH NADPH reactive oxygen species (ROS) |
| title | In Vivo Imaging with Genetically Encoded Redox Biosensors |
| title_full | In Vivo Imaging with Genetically Encoded Redox Biosensors |
| title_fullStr | In Vivo Imaging with Genetically Encoded Redox Biosensors |
| title_full_unstemmed | In Vivo Imaging with Genetically Encoded Redox Biosensors |
| title_short | In Vivo Imaging with Genetically Encoded Redox Biosensors |
| title_sort | in vivo imaging with genetically encoded redox biosensors |
| topic | fluorescent proteins genetically encoded sensors glutathione (GSH), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), in vivo imaging mycothiol (MSH), NADH NADPH reactive oxygen species (ROS) |
| url | https://www.mdpi.com/1422-0067/21/21/8164 |
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