Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China
ABSTRACTEmergence of multidrug-resistant (MDR) Salmonella enterica serovar Indiana (S. Indiana), a dominant Salmonella serovar in China, has raised global awareness because the MDR S. Indiana also was rapidly emerged in other countries recently. To improve our understanding of underlying MDR mechani...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
|
| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2018.1558961 |
| _version_ | 1797385761105903616 |
|---|---|
| author | Jiansen Gong Ximin Zeng Ping Zhang Di Zhang Chengming Wang Jun Lin |
| author_facet | Jiansen Gong Ximin Zeng Ping Zhang Di Zhang Chengming Wang Jun Lin |
| author_sort | Jiansen Gong |
| collection | DOAJ |
| description | ABSTRACTEmergence of multidrug-resistant (MDR) Salmonella enterica serovar Indiana (S. Indiana), a dominant Salmonella serovar in China, has raised global awareness because the MDR S. Indiana also was rapidly emerged in other countries recently. To improve our understanding of underlying MDR mechanism and evolution of this emerging zoonotic pathogen, here we examined the standard ATCC51959 strain together with 19 diverse and representative Chinese S. Indiana strains by performing comprehensive microbiological, molecular, and comparative genomics analyses. The findings from S1-PFGE, plasmid origin analysis and Southern blotting suggested the MDR phenotype in the majority of isolates was associated with large integron-carrying plasmids. Interestingly, further in-depth analyses of two recently isolated, plasmid-free MDR S. Indiana revealed a long chromosomal class I integron (7.8 kb) that is not linked to the Salmonella Genome Island 1 (SGI1), which is rare. This unique chromosomal integron shares extremely high similarity to that identified in a MDR E. coli plasmid pLM6771 with respect to both genomic organization and sequence identity. Taken together, both plasmid and chromosomal integron I exist in the examined MDR S. Indiana strains. This timely study represents a significant step toward the understanding of molecular basis of the emerging MDR S. Indiana. |
| first_indexed | 2024-03-08T21:58:55Z |
| format | Article |
| id | doaj.art-59bf7ffd25f9424a8f15b4bdac69fda3 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:58:55Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-59bf7ffd25f9424a8f15b4bdac69fda32023-12-19T16:09:58ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-0181293910.1080/22221751.2018.1558961Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in ChinaJiansen Gong0Ximin Zeng1Ping Zhang2Di Zhang3Chengming Wang4Jun Lin5Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, People’s Republic of ChinaDepartment of Animal Science, The University of Tennessee, Knoxville, TN, USAPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, People’s Republic of ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, People’s Republic of ChinaDepartment of Pathobiology, Auburn University College of Veterinary Medicine, Auburn, AL, USADepartment of Animal Science, The University of Tennessee, Knoxville, TN, USAABSTRACTEmergence of multidrug-resistant (MDR) Salmonella enterica serovar Indiana (S. Indiana), a dominant Salmonella serovar in China, has raised global awareness because the MDR S. Indiana also was rapidly emerged in other countries recently. To improve our understanding of underlying MDR mechanism and evolution of this emerging zoonotic pathogen, here we examined the standard ATCC51959 strain together with 19 diverse and representative Chinese S. Indiana strains by performing comprehensive microbiological, molecular, and comparative genomics analyses. The findings from S1-PFGE, plasmid origin analysis and Southern blotting suggested the MDR phenotype in the majority of isolates was associated with large integron-carrying plasmids. Interestingly, further in-depth analyses of two recently isolated, plasmid-free MDR S. Indiana revealed a long chromosomal class I integron (7.8 kb) that is not linked to the Salmonella Genome Island 1 (SGI1), which is rare. This unique chromosomal integron shares extremely high similarity to that identified in a MDR E. coli plasmid pLM6771 with respect to both genomic organization and sequence identity. Taken together, both plasmid and chromosomal integron I exist in the examined MDR S. Indiana strains. This timely study represents a significant step toward the understanding of molecular basis of the emerging MDR S. Indiana.https://www.tandfonline.com/doi/10.1080/22221751.2018.1558961Salmonella Indianaemerging pathogenmultidrug resistancemolecular basisintegron |
| spellingShingle | Jiansen Gong Ximin Zeng Ping Zhang Di Zhang Chengming Wang Jun Lin Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China Emerging Microbes and Infections Salmonella Indiana emerging pathogen multidrug resistance molecular basis integron |
| title | Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China |
| title_full | Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China |
| title_fullStr | Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China |
| title_full_unstemmed | Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China |
| title_short | Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China |
| title_sort | characterization of the emerging multidrug resistant salmonella enterica serovar indiana strains in china |
| topic | Salmonella Indiana emerging pathogen multidrug resistance molecular basis integron |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2018.1558961 |
| work_keys_str_mv | AT jiansengong characterizationoftheemergingmultidrugresistantsalmonellaentericaserovarindianastrainsinchina AT ximinzeng characterizationoftheemergingmultidrugresistantsalmonellaentericaserovarindianastrainsinchina AT pingzhang characterizationoftheemergingmultidrugresistantsalmonellaentericaserovarindianastrainsinchina AT dizhang characterizationoftheemergingmultidrugresistantsalmonellaentericaserovarindianastrainsinchina AT chengmingwang characterizationoftheemergingmultidrugresistantsalmonellaentericaserovarindianastrainsinchina AT junlin characterizationoftheemergingmultidrugresistantsalmonellaentericaserovarindianastrainsinchina |