Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio
Abstract Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine def...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-27612-5 |
| _version_ | 1797952547044982784 |
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| author | Catherine A. Richter Allison N. Evans Scott A. Heppell James L. Zajicek Donald E. Tillitt |
| author_facet | Catherine A. Richter Allison N. Evans Scott A. Heppell James L. Zajicek Donald E. Tillitt |
| author_sort | Catherine A. Richter |
| collection | DOAJ |
| description | Abstract Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality and recruitment failure in farmed animals and wild salmonine populations around the world. In the North American Great Lakes, consumption of the non-native prey fish alewife (Alosa pseudoharengus) by native lake trout (Salvelinus namaycush) led to thiamine deficiency in the trout, contributed to elevated fry mortality, and impeded natural population recruitment. Several thiaminases have been genetically characterized in bacteria and unicellular eukaryotes, and the source of thiaminase in multicellular organisms has been hypothesized to be gut microflora. In an unexpected discovery, we identified thiaminase I genes in zebrafish (Danio rerio) with homology to bacterial tenA thiaminase II. The biochemical activity of zebrafish thiaminase I (GenBank NP_001314821.1) was confirmed in a recombinant system. Genes homologous to the zebrafish tenA-like thiaminase I were identified in many animals, including common carp (Cyprinus carpio), zebra mussel (Dreissena polymorpha) and alewife. Thus, the source of thiaminase I in alewife impacting lake trout populations is likely to be de novo synthesis. |
| first_indexed | 2024-04-10T22:49:17Z |
| format | Article |
| id | doaj.art-59c6aa091f6d4138bb0dc275fd34e28a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T22:49:17Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-59c6aa091f6d4138bb0dc275fd34e28a2023-01-15T12:09:06ZengNature PortfolioScientific Reports2045-23222023-01-0113111010.1038/s41598-023-27612-5Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerioCatherine A. Richter0Allison N. Evans1Scott A. Heppell2James L. Zajicek3Donald E. Tillitt4U.S. Geological Survey, Columbia Environmental Research CenterDepartment of Fisheries, Wildlife, and Conservation Sciences, Oregon State UniversityDepartment of Fisheries, Wildlife, and Conservation Sciences, Oregon State UniversityU.S. Geological Survey, Columbia Environmental Research CenterU.S. Geological Survey, Columbia Environmental Research CenterAbstract Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality and recruitment failure in farmed animals and wild salmonine populations around the world. In the North American Great Lakes, consumption of the non-native prey fish alewife (Alosa pseudoharengus) by native lake trout (Salvelinus namaycush) led to thiamine deficiency in the trout, contributed to elevated fry mortality, and impeded natural population recruitment. Several thiaminases have been genetically characterized in bacteria and unicellular eukaryotes, and the source of thiaminase in multicellular organisms has been hypothesized to be gut microflora. In an unexpected discovery, we identified thiaminase I genes in zebrafish (Danio rerio) with homology to bacterial tenA thiaminase II. The biochemical activity of zebrafish thiaminase I (GenBank NP_001314821.1) was confirmed in a recombinant system. Genes homologous to the zebrafish tenA-like thiaminase I were identified in many animals, including common carp (Cyprinus carpio), zebra mussel (Dreissena polymorpha) and alewife. Thus, the source of thiaminase I in alewife impacting lake trout populations is likely to be de novo synthesis.https://doi.org/10.1038/s41598-023-27612-5 |
| spellingShingle | Catherine A. Richter Allison N. Evans Scott A. Heppell James L. Zajicek Donald E. Tillitt Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio Scientific Reports |
| title | Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio |
| title_full | Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio |
| title_fullStr | Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio |
| title_full_unstemmed | Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio |
| title_short | Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio |
| title_sort | genetic basis of thiaminase i activity in a vertebrate zebrafish danio rerio |
| url | https://doi.org/10.1038/s41598-023-27612-5 |
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