The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus
Rotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2004-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013 |
| _version_ | 1797757890173337600 |
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| author | Juliana Galera Castilho Maria Valéria Jacobucci Botelho Flávio Lauretti Noemi Taniwaki Rosa Elisa Carvalho Linhares Carlos Nozawa |
| author_facet | Juliana Galera Castilho Maria Valéria Jacobucci Botelho Flávio Lauretti Noemi Taniwaki Rosa Elisa Carvalho Linhares Carlos Nozawa |
| author_sort | Juliana Galera Castilho |
| collection | DOAJ |
| description | Rotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic disease. The current work describes the cytopathic changes in cultured MA-104 cells infected by a simian (SA-11) and a porcine (1154) rotavirus strains. Trypan blue exclusion staining showed increased cell permeability after infection by both strains, as demonstrated by cell viability. This effect was confirmed by the leakage of infected cells evaluated by chromium release. Nuclear fragmentation was observed by acridine orange and Wright staining but specific DNA cleavage was not detected. Ultrastructural changes, such as chromatin condensation, cytoplasm vacuolisation, and loss of intercellular contact were shown in infected cells for both strains. In situ terminal deoxynucleotidyl transferase (Tunel) assay did not show positive result. In conclusion, we demonstrated that both strains of rotavirus induced necrosis as the major degenerative effect. |
| first_indexed | 2024-03-12T18:22:04Z |
| format | Article |
| id | doaj.art-59c8985055d4481fb740601da8397255 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:22:04Z |
| publishDate | 2004-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-59c8985055d4481fb740601da83972552023-08-02T08:46:56ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602004-05-0199331331710.1590/S0074-02762004000300013The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirusJuliana Galera CastilhoMaria Valéria Jacobucci BotelhoFlávio LaurettiNoemi TaniwakiRosa Elisa Carvalho LinharesCarlos NozawaRotaviruses have been implicated as the major causal agents of acute diarrhoea in mammals and fowls. Experimental rotavirus infection have been associated to a series of sub-cellular pathologic alterations leading to cell lysis which may represent key functions in the pathogenesis of the diarrhoeic disease. The current work describes the cytopathic changes in cultured MA-104 cells infected by a simian (SA-11) and a porcine (1154) rotavirus strains. Trypan blue exclusion staining showed increased cell permeability after infection by both strains, as demonstrated by cell viability. This effect was confirmed by the leakage of infected cells evaluated by chromium release. Nuclear fragmentation was observed by acridine orange and Wright staining but specific DNA cleavage was not detected. Ultrastructural changes, such as chromatin condensation, cytoplasm vacuolisation, and loss of intercellular contact were shown in infected cells for both strains. In situ terminal deoxynucleotidyl transferase (Tunel) assay did not show positive result. In conclusion, we demonstrated that both strains of rotavirus induced necrosis as the major degenerative effect.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013rotaviruscytopathologynecrosisapoptosiscell culture |
| spellingShingle | Juliana Galera Castilho Maria Valéria Jacobucci Botelho Flávio Lauretti Noemi Taniwaki Rosa Elisa Carvalho Linhares Carlos Nozawa The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus Memorias do Instituto Oswaldo Cruz rotavirus cytopathology necrosis apoptosis cell culture |
| title | The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus |
| title_full | The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus |
| title_fullStr | The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus |
| title_full_unstemmed | The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus |
| title_short | The in vitro cytopathology of a porcine and the simian (SA-11) strains of rotavirus |
| title_sort | in vitro cytopathology of a porcine and the simian sa 11 strains of rotavirus |
| topic | rotavirus cytopathology necrosis apoptosis cell culture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000300013 |
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