Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks
Perkinsus species, which are parasitic pathogens of mollusks, have been transmitted and dispersed to various molluscan species along the coastal waters of many countries. However, few studies have addressed the diversity and distribution of Perkinsus spp. along the coast of China. Here we used conve...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.989261/full |
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| author | Lingtong Ye Lingtong Ye Lin Wu Lin Wu Jie Lu Jie Lu Tuo Yao Tuo Yao Jiangyong Wang Shaokun Shi Gang Yu Wang Zhao |
| author_facet | Lingtong Ye Lingtong Ye Lin Wu Lin Wu Jie Lu Jie Lu Tuo Yao Tuo Yao Jiangyong Wang Shaokun Shi Gang Yu Wang Zhao |
| author_sort | Lingtong Ye |
| collection | DOAJ |
| description | Perkinsus species, which are parasitic pathogens of mollusks, have been transmitted and dispersed to various molluscan species along the coastal waters of many countries. However, few studies have addressed the diversity and distribution of Perkinsus spp. along the coast of China. Here we used conventional PCR amplification and sequencing techniques, combined with a qPCR assay as a confirmatory test, to evaluate the prevalence of Perkinsus species in molluscan species among different sea regions of China. Three Perkinsus species—P. olseni, P. beihaiensis, and P. chesapeaki—were detected, with P. chesapeaki reported for the first time along the Chinese coast. Seven of eight molluscan species carried Perkinsus species, including Crassostrea gigas, C. hongkongensis, Sinonovacula constricta, Ruditapes philippinarum, Scapharca subcrenata, Meretrix lyrate, and Haliotis diversicolor. Perkinsus olseni was prominent in the Yellow and Bohai Sea and East China Sea, while P. beihaiensis was prominent in the South China Sea. Most of the molluscan species carried Perkinsus spp. with the medium or low levels of PCR-prevalence (<30%). The three Perkinsus species possess high levels of internal transcriber spacer haplotypes, some of which are shared among many countries. The much higher PCR-prevalence of Perkinsus spp. in the clam Ruditapes philippinarum and the oyster Crassostrea hongkongensis suggests that Perkinsus species may be transmitted and dispersed to other mollusks through the transportation of Perkinsus-carried R. philippinarum and C. hongkongensis. Perkinsus carrying tended to be generally linked with a broader geographic range, lower prevalence, more diversified molluscan hosts, and more diversified Perkinsus haplotypes. |
| first_indexed | 2024-04-11T10:02:49Z |
| format | Article |
| id | doaj.art-59cb4c21dbc54d47a49c30f34796cc1f |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-11T10:02:49Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-59cb4c21dbc54d47a49c30f34796cc1f2022-12-22T04:30:19ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-10-01910.3389/fmars.2022.989261989261Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusksLingtong Ye0Lingtong Ye1Lin Wu2Lin Wu3Jie Lu4Jie Lu5Tuo Yao6Tuo Yao7Jiangyong Wang8Shaokun Shi9Gang Yu10Wang Zhao11Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSchool of Life Science, Huizhou University, Huizhou, ChinaShenzhen Fisheries Development Research Center, Shenzhen, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaPerkinsus species, which are parasitic pathogens of mollusks, have been transmitted and dispersed to various molluscan species along the coastal waters of many countries. However, few studies have addressed the diversity and distribution of Perkinsus spp. along the coast of China. Here we used conventional PCR amplification and sequencing techniques, combined with a qPCR assay as a confirmatory test, to evaluate the prevalence of Perkinsus species in molluscan species among different sea regions of China. Three Perkinsus species—P. olseni, P. beihaiensis, and P. chesapeaki—were detected, with P. chesapeaki reported for the first time along the Chinese coast. Seven of eight molluscan species carried Perkinsus species, including Crassostrea gigas, C. hongkongensis, Sinonovacula constricta, Ruditapes philippinarum, Scapharca subcrenata, Meretrix lyrate, and Haliotis diversicolor. Perkinsus olseni was prominent in the Yellow and Bohai Sea and East China Sea, while P. beihaiensis was prominent in the South China Sea. Most of the molluscan species carried Perkinsus spp. with the medium or low levels of PCR-prevalence (<30%). The three Perkinsus species possess high levels of internal transcriber spacer haplotypes, some of which are shared among many countries. The much higher PCR-prevalence of Perkinsus spp. in the clam Ruditapes philippinarum and the oyster Crassostrea hongkongensis suggests that Perkinsus species may be transmitted and dispersed to other mollusks through the transportation of Perkinsus-carried R. philippinarum and C. hongkongensis. Perkinsus carrying tended to be generally linked with a broader geographic range, lower prevalence, more diversified molluscan hosts, and more diversified Perkinsus haplotypes.https://www.frontiersin.org/articles/10.3389/fmars.2022.989261/fullPerkinsus spp. distribution in China PerkinsusmollusksdiversitydistributionPerkinsus chesapeakiChinese coast |
| spellingShingle | Lingtong Ye Lingtong Ye Lin Wu Lin Wu Jie Lu Jie Lu Tuo Yao Tuo Yao Jiangyong Wang Shaokun Shi Gang Yu Wang Zhao Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks Frontiers in Marine Science Perkinsus spp. distribution in China Perkinsus mollusks diversity distribution Perkinsus chesapeaki Chinese coast |
| title | Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks |
| title_full | Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks |
| title_fullStr | Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks |
| title_full_unstemmed | Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks |
| title_short | Diversity and distribution of Perkinsus spp. along the coast of China: Implications for widespread transmission of Perkinsus spp. in mollusks |
| title_sort | diversity and distribution of perkinsus spp along the coast of china implications for widespread transmission of perkinsus spp in mollusks |
| topic | Perkinsus spp. distribution in China Perkinsus mollusks diversity distribution Perkinsus chesapeaki Chinese coast |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.989261/full |
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