Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture
The study of exogenous metabolites of algae-bacterial communities in the laboratory accumulative culture obtained from natural river water was conducted using gas chromatography-mass spectrometry. Exometabolites of the algae-bacterial community (including microalgae and cyanobacteria) in the culture...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/15/22/3879 |
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| author | Evgeny A. Kurashov Yulia V. Bataeva Julia V. Krylova Ivan A. Dyatlov |
| author_facet | Evgeny A. Kurashov Yulia V. Bataeva Julia V. Krylova Ivan A. Dyatlov |
| author_sort | Evgeny A. Kurashov |
| collection | DOAJ |
| description | The study of exogenous metabolites of algae-bacterial communities in the laboratory accumulative culture obtained from natural river water was conducted using gas chromatography-mass spectrometry. Exometabolites of the algae-bacterial community (including microalgae and cyanobacteria) in the culture medium were represented by saturated, unsaturated, and aromatic hydrocarbons, carboxylic acids, phenolic, and terpene compounds and their derivatives. Possible biological activities of the discovered exometabolites are considered. The study has demonstrated that an increase in the number of main groups of microorganisms, along with changes in the composition of algae and cyanobacteria, are responsible for the increase in the composition and concentration of metabolites in the microecosystem’s culture medium after one month of cultivation. The presence of octacosane in high concentration (0.0603 mg/L; 23.78% of the total content of low molecular weight organic compounds) by the end of exposure accumulative culture is associated with the strong development of the cyanobacterium <i>Gloeocapsa</i> sp. in the presence of diatom algae of the genus <i>Navicula</i> and green algae of the genera <i>Chlorella</i> and <i>Scenedesmus</i>. Due to the need to comprehend the ecological and biochemical mechanisms of the formation and functioning of algae-bacterial communities, as well as to predict potential paths of transformation and evolution of aquatic ecosystems, the specificity of exometabolite complexes of algae and microorganisms, as well as their allelopathic and other biochemical interactions in freshwater ecosystems, requires further serious study. |
| first_indexed | 2024-03-09T16:22:36Z |
| format | Article |
| id | doaj.art-59cddd26818a42f4ae5aebdbc458ece1 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T16:22:36Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-59cddd26818a42f4ae5aebdbc458ece12023-11-24T15:11:07ZengMDPI AGWater2073-44412023-11-011522387910.3390/w15223879Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative CultureEvgeny A. Kurashov0Yulia V. Bataeva1Julia V. Krylova2Ivan A. Dyatlov3Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742 Borok, RussiaState Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, RussiaPapanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742 Borok, RussiaState Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, RussiaThe study of exogenous metabolites of algae-bacterial communities in the laboratory accumulative culture obtained from natural river water was conducted using gas chromatography-mass spectrometry. Exometabolites of the algae-bacterial community (including microalgae and cyanobacteria) in the culture medium were represented by saturated, unsaturated, and aromatic hydrocarbons, carboxylic acids, phenolic, and terpene compounds and their derivatives. Possible biological activities of the discovered exometabolites are considered. The study has demonstrated that an increase in the number of main groups of microorganisms, along with changes in the composition of algae and cyanobacteria, are responsible for the increase in the composition and concentration of metabolites in the microecosystem’s culture medium after one month of cultivation. The presence of octacosane in high concentration (0.0603 mg/L; 23.78% of the total content of low molecular weight organic compounds) by the end of exposure accumulative culture is associated with the strong development of the cyanobacterium <i>Gloeocapsa</i> sp. in the presence of diatom algae of the genus <i>Navicula</i> and green algae of the genera <i>Chlorella</i> and <i>Scenedesmus</i>. Due to the need to comprehend the ecological and biochemical mechanisms of the formation and functioning of algae-bacterial communities, as well as to predict potential paths of transformation and evolution of aquatic ecosystems, the specificity of exometabolite complexes of algae and microorganisms, as well as their allelopathic and other biochemical interactions in freshwater ecosystems, requires further serious study.https://www.mdpi.com/2073-4441/15/22/3879algaecyanobacteriaexometabolitesgas chromatography-mass spectrometryalgae-bacterial communitiesaccumulative culture |
| spellingShingle | Evgeny A. Kurashov Yulia V. Bataeva Julia V. Krylova Ivan A. Dyatlov Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture Water algae cyanobacteria exometabolites gas chromatography-mass spectrometry algae-bacterial communities accumulative culture |
| title | Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture |
| title_full | Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture |
| title_fullStr | Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture |
| title_full_unstemmed | Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture |
| title_short | Gas Chromatography-Mass Spectrometric Study of Low-Molecular-Weight Exogenous Metabolites of Algae-Bacterial Communities in the Laboratory Accumulative Culture |
| title_sort | gas chromatography mass spectrometric study of low molecular weight exogenous metabolites of algae bacterial communities in the laboratory accumulative culture |
| topic | algae cyanobacteria exometabolites gas chromatography-mass spectrometry algae-bacterial communities accumulative culture |
| url | https://www.mdpi.com/2073-4441/15/22/3879 |
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