Microbiological synthesis of methyl ethyl ketone
Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins’ medium con...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2016-01-01
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| Series: | MATEC Web of Conferences |
| Online Access: | http://dx.doi.org/10.1051/matecconf/20168501022 |
| _version_ | 1818351615519752192 |
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| author | Astashkina Anna Kolbysheva Yuliya Nikiforova Alena Bakibayev Abdigali |
| author_facet | Astashkina Anna Kolbysheva Yuliya Nikiforova Alena Bakibayev Abdigali |
| author_sort | Astashkina Anna |
| collection | DOAJ |
| description | Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins’ medium containing 3% (vol.) of the substrate, stirring speed – 80-100 rev/min, temperature – 37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%. |
| first_indexed | 2024-12-13T18:40:33Z |
| format | Article |
| id | doaj.art-59cf6d37b72d477d8d56a86df2776ea2 |
| institution | Directory Open Access Journal |
| issn | 2261-236X |
| language | English |
| last_indexed | 2024-12-13T18:40:33Z |
| publishDate | 2016-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | MATEC Web of Conferences |
| spelling | doaj.art-59cf6d37b72d477d8d56a86df2776ea22022-12-21T23:35:15ZengEDP SciencesMATEC Web of Conferences2261-236X2016-01-01850102210.1051/matecconf/20168501022matecconf_cct2016_01022Microbiological synthesis of methyl ethyl ketoneAstashkina AnnaKolbysheva YuliyaNikiforova AlenaBakibayev AbdigaliOptimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins’ medium containing 3% (vol.) of the substrate, stirring speed – 80-100 rev/min, temperature – 37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.http://dx.doi.org/10.1051/matecconf/20168501022 |
| spellingShingle | Astashkina Anna Kolbysheva Yuliya Nikiforova Alena Bakibayev Abdigali Microbiological synthesis of methyl ethyl ketone MATEC Web of Conferences |
| title | Microbiological synthesis of methyl ethyl ketone |
| title_full | Microbiological synthesis of methyl ethyl ketone |
| title_fullStr | Microbiological synthesis of methyl ethyl ketone |
| title_full_unstemmed | Microbiological synthesis of methyl ethyl ketone |
| title_short | Microbiological synthesis of methyl ethyl ketone |
| title_sort | microbiological synthesis of methyl ethyl ketone |
| url | http://dx.doi.org/10.1051/matecconf/20168501022 |
| work_keys_str_mv | AT astashkinaanna microbiologicalsynthesisofmethylethylketone AT kolbyshevayuliya microbiologicalsynthesisofmethylethylketone AT nikiforovaalena microbiologicalsynthesisofmethylethylketone AT bakibayevabdigali microbiologicalsynthesisofmethylethylketone |