Baroreflex Modulation During Acute High-Altitude Exposure in Rats
Baroreflex (BR) control is critically dependent of sympathetic and parasympathetic modulation. It has been documented that during acute hypobaric hypoxia there is a BR control impairment, however, the effect of a natural hypoxic environment on BR function is limited and controversial. Therefore, the...
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| Format: | Article |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.01049/full |
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| author | Ana Rosa Beltrán Ana Rosa Beltrán Alexis Arce-Álvarez Rodrigo Ramirez-Campillo Rodrigo Ramirez-Campillo Manuel Vásquez-Muñoz Magdalena von Igel Marco A. Ramírez Rodrigo Del Rio Rodrigo Del Rio Rodrigo Del Rio David C. Andrade David C. Andrade David C. Andrade |
| author_facet | Ana Rosa Beltrán Ana Rosa Beltrán Alexis Arce-Álvarez Rodrigo Ramirez-Campillo Rodrigo Ramirez-Campillo Manuel Vásquez-Muñoz Magdalena von Igel Marco A. Ramírez Rodrigo Del Rio Rodrigo Del Rio Rodrigo Del Rio David C. Andrade David C. Andrade David C. Andrade |
| author_sort | Ana Rosa Beltrán |
| collection | DOAJ |
| description | Baroreflex (BR) control is critically dependent of sympathetic and parasympathetic modulation. It has been documented that during acute hypobaric hypoxia there is a BR control impairment, however, the effect of a natural hypoxic environment on BR function is limited and controversial. Therefore, the aim of this study was to determine the effect of acute High-Altitude exposure on sympathetic/parasympathetic modulation of BR control in normal rats. Male Sprague Dawley rats were randomly allocated into Sea-Level (n = 7) and High-Altitude (n = 5) (3,270 m above sea level) groups. The BR control was studied using phenylephrine (Phe) and sodium nitroprusside (SNP) through sigmoidal analysis. The autonomic control of the heart was estimated using heart rate variability (HRV) analysis in frequency domain. Additionally, to determine the maximum sympathetic and parasympathetic activation of BR, spectral non-stationary method analysis, during Phe (0.05 μg/mL) and SNP administration (0.10 μg/mL) were used. Compared to Sea-Level condition, the High-Altitude group displayed parasympathetic withdrawal (high frequency, 0.6–2.4 Hz) and sympathoexcitation (low frequency, 0.04–0.6 Hz). Regarding to BR modulation, rats showed a significant decrease (p < 0.05) of curvature and parasympathetic bradycardic responses to Phe, without significant differences in sympathetic tachycardic responses to SNP after High-Altitude exposure. In addition, the non-stationary analysis of HRV showed a reduction of parasympathetic activation (Phe) in the High-Altitude group. Our results suggest that acute exposure to High-Altitude produces an autonomic and BR control impairment, characterized by parasympathetic withdrawal after 24 h of high-altitude exposure. |
| first_indexed | 2024-12-19T15:03:18Z |
| format | Article |
| id | doaj.art-59cfba1a4ec64cd7a83aade9f31b1699 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-19T15:03:18Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-59cfba1a4ec64cd7a83aade9f31b16992022-12-21T20:16:30ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-08-011110.3389/fphys.2020.01049539864Baroreflex Modulation During Acute High-Altitude Exposure in RatsAna Rosa Beltrán0Ana Rosa Beltrán1Alexis Arce-Álvarez2Rodrigo Ramirez-Campillo3Rodrigo Ramirez-Campillo4Manuel Vásquez-Muñoz5Magdalena von Igel6Marco A. Ramírez7Rodrigo Del Rio8Rodrigo Del Rio9Rodrigo Del Rio10David C. Andrade11David C. Andrade12David C. Andrade13Departamento de Educación, Facultad de Educación, Universidad de Antofagasta, Antofagasta, ChileLaboratorio de Fisiología Celular, Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, ChileEscuela de Kinesiología, Facultad de Salud, Universidad Católica Silva Henríquez, Santiago, ChileLaboratory of Human Performance, Quality of Life and Wellness Research Group, Department of Physical Activity Sciences, Universidad de Los Lagos, Osorno, ChileCentro de Investigación en Fisiología del Ejercicio, Facultad de Ciencias, Universidad Mayor, Santiago, ChileCentro de Investigación en Fisiología del Ejercicio, Facultad de Ciencias, Universidad Mayor, Santiago, ChileCentro de Investigación en Fisiología del Ejercicio, Facultad de Ciencias, Universidad Mayor, Santiago, ChileLaboratorio de Fisiología Celular, Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, ChileLaboratory of Cardiorespiratory Control, Department of Physiology, Pontificia Universidad Católica de Chile, Santiago, ChileCentro de Envejecimiento y Regeneración (CARE), Pontificia Universidad Católica de Chile, Santiago, ChileCentro de Excelencia en Biomedicina de Magallanes (CEBIMA), Universidad de Magallanes, Punta Arenas, ChileCentro de Investigación en Fisiología del Ejercicio, Facultad de Ciencias, Universidad Mayor, Santiago, ChileLaboratory of Cardiorespiratory Control, Department of Physiology, Pontificia Universidad Católica de Chile, Santiago, ChilePedagogía en Educación Física, Deportes y Recreación, Universidad Mayor, Santiago, ChileBaroreflex (BR) control is critically dependent of sympathetic and parasympathetic modulation. It has been documented that during acute hypobaric hypoxia there is a BR control impairment, however, the effect of a natural hypoxic environment on BR function is limited and controversial. Therefore, the aim of this study was to determine the effect of acute High-Altitude exposure on sympathetic/parasympathetic modulation of BR control in normal rats. Male Sprague Dawley rats were randomly allocated into Sea-Level (n = 7) and High-Altitude (n = 5) (3,270 m above sea level) groups. The BR control was studied using phenylephrine (Phe) and sodium nitroprusside (SNP) through sigmoidal analysis. The autonomic control of the heart was estimated using heart rate variability (HRV) analysis in frequency domain. Additionally, to determine the maximum sympathetic and parasympathetic activation of BR, spectral non-stationary method analysis, during Phe (0.05 μg/mL) and SNP administration (0.10 μg/mL) were used. Compared to Sea-Level condition, the High-Altitude group displayed parasympathetic withdrawal (high frequency, 0.6–2.4 Hz) and sympathoexcitation (low frequency, 0.04–0.6 Hz). Regarding to BR modulation, rats showed a significant decrease (p < 0.05) of curvature and parasympathetic bradycardic responses to Phe, without significant differences in sympathetic tachycardic responses to SNP after High-Altitude exposure. In addition, the non-stationary analysis of HRV showed a reduction of parasympathetic activation (Phe) in the High-Altitude group. Our results suggest that acute exposure to High-Altitude produces an autonomic and BR control impairment, characterized by parasympathetic withdrawal after 24 h of high-altitude exposure.https://www.frontiersin.org/article/10.3389/fphys.2020.01049/fullbaroreflexautonomic nervous systemsympathetic nervous systemparasympathetic nervous systembaroreceptors |
| spellingShingle | Ana Rosa Beltrán Ana Rosa Beltrán Alexis Arce-Álvarez Rodrigo Ramirez-Campillo Rodrigo Ramirez-Campillo Manuel Vásquez-Muñoz Magdalena von Igel Marco A. Ramírez Rodrigo Del Rio Rodrigo Del Rio Rodrigo Del Rio David C. Andrade David C. Andrade David C. Andrade Baroreflex Modulation During Acute High-Altitude Exposure in Rats Frontiers in Physiology baroreflex autonomic nervous system sympathetic nervous system parasympathetic nervous system baroreceptors |
| title | Baroreflex Modulation During Acute High-Altitude Exposure in Rats |
| title_full | Baroreflex Modulation During Acute High-Altitude Exposure in Rats |
| title_fullStr | Baroreflex Modulation During Acute High-Altitude Exposure in Rats |
| title_full_unstemmed | Baroreflex Modulation During Acute High-Altitude Exposure in Rats |
| title_short | Baroreflex Modulation During Acute High-Altitude Exposure in Rats |
| title_sort | baroreflex modulation during acute high altitude exposure in rats |
| topic | baroreflex autonomic nervous system sympathetic nervous system parasympathetic nervous system baroreceptors |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.01049/full |
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