Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus)
Abstract: Sleep deprivation-induced stress decreases the quality of spermatozoa due to the increase of glucocorticoid levels through the hypothalamic-pituitary-adrenal axis. Sleep recovery (SR) restores the effect of stress due to sleep deprivation by decreasing lipid peroxides and other free radic...
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| Format: | Article |
| Language: | English |
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Universitas Lambung Mangkurat
2023-02-01
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| Series: | Berkala Kedokteran |
| Subjects: | |
| Online Access: | https://ppjp.ulm.ac.id/journal/index.php/jbk/article/view/15719 |
| _version_ | 1797315585331167232 |
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| author | Fitranto Arjadi Sindhu Wisesa Vitasari Indriani |
| author_facet | Fitranto Arjadi Sindhu Wisesa Vitasari Indriani |
| author_sort | Fitranto Arjadi |
| collection | DOAJ |
| description | Abstract:
Sleep deprivation-induced stress decreases the quality of spermatozoa due to the increase of glucocorticoid levels through the hypothalamic-pituitary-adrenal axis. Sleep recovery (SR) restores the effect of stress due to sleep deprivation by decreasing lipid peroxides and other free radicals and increasing glutathione antioxidants. This study aims to determine the spermatozoa quality in male albino rats (Rattus norvegicus) after the stress induction by diverse sleep deprivation protocols. This study is experimental research with posttest-only control group design in 30 male albino rats divided into five groups consisting of group I for negative control (no induction of sleep deprivation), group II for paradoxical sleep deprivation (PSD), group III for total sleep deprivation (TSD), group IV for PSD followed by SR, and group V for TSD followed by SR. PSD and TSD were performed by depriving rats sleep for 20 and 24 hours/day, respectively, for five days. SR was done for the next five days after PSD or TSD. Shapiro-Wilk test was used for normality of data distribution, and Levene's test was used for variance homogeneity. Comparative hypothesis was then analyzed with One Way Analysis of Variance (ANOVA) test, and significance between groups was analyzed with Tukey's Post-Hoc test. Group III (TSD) demonstrated the lowest mean of spermatozoa count (41,53±2,29), spermatozoa morphology (13,00±2,91), spermatozoa viability (31,00±7,39), and percentage of motility rate (37±8,58%) among all groups. In conclusion, total sleep deprivation (TSD) decreases the quality of spermatozoa in male albino rats (Rattus norvegicus). |
| first_indexed | 2024-03-08T03:05:52Z |
| format | Article |
| id | doaj.art-59d0516908ff4b04bd1bfdb2fa9f7ded |
| institution | Directory Open Access Journal |
| issn | 1412-0550 2548-5660 |
| language | English |
| last_indexed | 2024-03-08T03:05:52Z |
| publishDate | 2023-02-01 |
| publisher | Universitas Lambung Mangkurat |
| record_format | Article |
| series | Berkala Kedokteran |
| spelling | doaj.art-59d0516908ff4b04bd1bfdb2fa9f7ded2024-02-13T09:27:32ZengUniversitas Lambung MangkuratBerkala Kedokteran1412-05502548-56602023-02-01191697810.20527/jbk.v19i1.157198370Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus)Fitranto Arjadi0Sindhu Wisesa1Vitasari Indriani2Jenderal Soedirman UniversityJenderal Soedirman UniversityJenderal Soedirman UniversityAbstract: Sleep deprivation-induced stress decreases the quality of spermatozoa due to the increase of glucocorticoid levels through the hypothalamic-pituitary-adrenal axis. Sleep recovery (SR) restores the effect of stress due to sleep deprivation by decreasing lipid peroxides and other free radicals and increasing glutathione antioxidants. This study aims to determine the spermatozoa quality in male albino rats (Rattus norvegicus) after the stress induction by diverse sleep deprivation protocols. This study is experimental research with posttest-only control group design in 30 male albino rats divided into five groups consisting of group I for negative control (no induction of sleep deprivation), group II for paradoxical sleep deprivation (PSD), group III for total sleep deprivation (TSD), group IV for PSD followed by SR, and group V for TSD followed by SR. PSD and TSD were performed by depriving rats sleep for 20 and 24 hours/day, respectively, for five days. SR was done for the next five days after PSD or TSD. Shapiro-Wilk test was used for normality of data distribution, and Levene's test was used for variance homogeneity. Comparative hypothesis was then analyzed with One Way Analysis of Variance (ANOVA) test, and significance between groups was analyzed with Tukey's Post-Hoc test. Group III (TSD) demonstrated the lowest mean of spermatozoa count (41,53±2,29), spermatozoa morphology (13,00±2,91), spermatozoa viability (31,00±7,39), and percentage of motility rate (37±8,58%) among all groups. In conclusion, total sleep deprivation (TSD) decreases the quality of spermatozoa in male albino rats (Rattus norvegicus).https://ppjp.ulm.ac.id/journal/index.php/jbk/article/view/15719albino ratquality of spermatozoatotal sleep deprivation |
| spellingShingle | Fitranto Arjadi Sindhu Wisesa Vitasari Indriani Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) Berkala Kedokteran albino rat quality of spermatozoa total sleep deprivation |
| title | Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) |
| title_full | Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) |
| title_fullStr | Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) |
| title_full_unstemmed | Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) |
| title_short | Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) |
| title_sort | total sleep deprivation reduces quality of spermatozoa in male albino rat rattus norvegicus |
| topic | albino rat quality of spermatozoa total sleep deprivation |
| url | https://ppjp.ulm.ac.id/journal/index.php/jbk/article/view/15719 |
| work_keys_str_mv | AT fitrantoarjadi totalsleepdeprivationreducesqualityofspermatozoainmalealbinoratrattusnorvegicus AT sindhuwisesa totalsleepdeprivationreducesqualityofspermatozoainmalealbinoratrattusnorvegicus AT vitasariindriani totalsleepdeprivationreducesqualityofspermatozoainmalealbinoratrattusnorvegicus |