Abnormalities of glucose metabolism in spontaneously hypertensive rats
Abnormalities in glucose metabolism and insulin action are frequently detected in patients with essential hypertension. Spontaneously hypertensive rats (SHR) have been used as an experimental model to understand this pathological condition. The objective of the present study was to assess glucose me...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2000-11-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100015 |
| _version_ | 1818291810236104704 |
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| author | L.M.F.B. Gouveia I.C. Kettelhut M.C. Foss |
| author_facet | L.M.F.B. Gouveia I.C. Kettelhut M.C. Foss |
| author_sort | L.M.F.B. Gouveia |
| collection | DOAJ |
| description | Abnormalities in glucose metabolism and insulin action are frequently detected in patients with essential hypertension. Spontaneously hypertensive rats (SHR) have been used as an experimental model to understand this pathological condition. The objective of the present study was to assess glucose metabolism and insulin action in SHR and Wistar rats under fed and fasting conditions. Peripheral glucose utilization was estimated by kinetic studies with [6-³H]-glucose and gluconeogenetic activity was measured during continuous [14C]-bicarbonate infusion. Plasma glucose levels were higher in the SHR group. Plasma insulin levels in the fed state were higher in the SHR group (99.8 ± 6.5 µM) than in the control group (70.4 ± 3.6 µM). Muscle glycogen content was reduced in SHR compared to control under the various experimental conditions. Peripheral glucose utilization was slightly lower in the SHR group in the fed state (8.72 ± 0.55 vs 9.52 ± 0.80 mg kg-1 min-1 in controls). Serum free fatty acid levels, hepatic glycogen levels, hepatic phosphoenolpyruvate carboxykinase activity and gluconeogenetic activity were similar in the two groups. The presence of hyperglycemia and hyperinsulinemia and the slightly reduced peripheral glucose utilization suggest the presence of resistance to the action of insulin in peripheral tissues of SHR. Hepatic gluconeogenesis does not seem to contribute to the metabolic alterations detected in these animals. |
| first_indexed | 2024-12-13T02:49:59Z |
| format | Article |
| id | doaj.art-59d0595dc98a46b0a842d08d1343e7f2 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-13T02:49:59Z |
| publishDate | 2000-11-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-59d0595dc98a46b0a842d08d1343e7f22022-12-22T00:02:04ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2000-11-0133111357136210.1590/S0100-879X2000001100015Abnormalities of glucose metabolism in spontaneously hypertensive ratsL.M.F.B. GouveiaI.C. KettelhutM.C. FossAbnormalities in glucose metabolism and insulin action are frequently detected in patients with essential hypertension. Spontaneously hypertensive rats (SHR) have been used as an experimental model to understand this pathological condition. The objective of the present study was to assess glucose metabolism and insulin action in SHR and Wistar rats under fed and fasting conditions. Peripheral glucose utilization was estimated by kinetic studies with [6-³H]-glucose and gluconeogenetic activity was measured during continuous [14C]-bicarbonate infusion. Plasma glucose levels were higher in the SHR group. Plasma insulin levels in the fed state were higher in the SHR group (99.8 ± 6.5 µM) than in the control group (70.4 ± 3.6 µM). Muscle glycogen content was reduced in SHR compared to control under the various experimental conditions. Peripheral glucose utilization was slightly lower in the SHR group in the fed state (8.72 ± 0.55 vs 9.52 ± 0.80 mg kg-1 min-1 in controls). Serum free fatty acid levels, hepatic glycogen levels, hepatic phosphoenolpyruvate carboxykinase activity and gluconeogenetic activity were similar in the two groups. The presence of hyperglycemia and hyperinsulinemia and the slightly reduced peripheral glucose utilization suggest the presence of resistance to the action of insulin in peripheral tissues of SHR. Hepatic gluconeogenesis does not seem to contribute to the metabolic alterations detected in these animals.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100015peripheral glucose utilizationgluconeogenesisinsulin resistancespontaneously hypertensive rats |
| spellingShingle | L.M.F.B. Gouveia I.C. Kettelhut M.C. Foss Abnormalities of glucose metabolism in spontaneously hypertensive rats Brazilian Journal of Medical and Biological Research peripheral glucose utilization gluconeogenesis insulin resistance spontaneously hypertensive rats |
| title | Abnormalities of glucose metabolism in spontaneously hypertensive rats |
| title_full | Abnormalities of glucose metabolism in spontaneously hypertensive rats |
| title_fullStr | Abnormalities of glucose metabolism in spontaneously hypertensive rats |
| title_full_unstemmed | Abnormalities of glucose metabolism in spontaneously hypertensive rats |
| title_short | Abnormalities of glucose metabolism in spontaneously hypertensive rats |
| title_sort | abnormalities of glucose metabolism in spontaneously hypertensive rats |
| topic | peripheral glucose utilization gluconeogenesis insulin resistance spontaneously hypertensive rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100015 |
| work_keys_str_mv | AT lmfbgouveia abnormalitiesofglucosemetabolisminspontaneouslyhypertensiverats AT ickettelhut abnormalitiesofglucosemetabolisminspontaneouslyhypertensiverats AT mcfoss abnormalitiesofglucosemetabolisminspontaneouslyhypertensiverats |