Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls
Abstract Rice (Oryza sativa L.) grain hull color is an easily observable trait and regarded as a crucial morphological marker in rice breeding. Here, a purple gradient grain hull mutant (pg) was found from natural mutations of a straw-white grain hull rice variety IARI 6184B (Orzya sativa L. subsp....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-08-01
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| Series: | Rice |
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| Online Access: | https://doi.org/10.1186/s12284-022-00589-x |
| _version_ | 1828154186917740544 |
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| author | Fantao Zhang Limin Yang Wenxue Huang Xiangdong Luo Jiankun Xie Biaolin Hu Yaling Chen |
| author_facet | Fantao Zhang Limin Yang Wenxue Huang Xiangdong Luo Jiankun Xie Biaolin Hu Yaling Chen |
| author_sort | Fantao Zhang |
| collection | DOAJ |
| description | Abstract Rice (Oryza sativa L.) grain hull color is an easily observable trait and regarded as a crucial morphological marker in rice breeding. Here, a purple gradient grain hull mutant (pg) was found from natural mutations of a straw-white grain hull rice variety IARI 6184B (Orzya sativa L. subsp. indica). The color of the mutant grain hulls changed from straw-white to pink, then purple, and finally brownish-yellow. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) identified 217 flavonoids, including 18 anthocyanins, among which cyanidin O-syringic acid had the highest concentration in pink (66.2 × 106) and purple (68.0 × 106) grain hulls. The relative contents of hesperetin O-malonyl-hexoside, apigenin derivatives, genistein derivatives, and kaempferol 3-O derivatives were consistently downregulated during pg grain hull development. Conversely, 12 anthocyanins were upregulated in colored hulls, and cyanidin 3-O-malonylhexoside was abundant only in pink and purple grain hulls. Moreover, the candidate gene was mapped into a 1.38 Mb region on chromosome 4 through bulked segregant analysis based on deep sequencing (BSA-seq) and gene mapping approaches. These results increased our understanding of anthocyanin biosynthesis in rice grains, helping rice breeders to select new rice varieties with desirable grain traits. |
| first_indexed | 2024-04-11T22:36:39Z |
| format | Article |
| id | doaj.art-59d0c85ce45b4387879dd48fffc4f0a9 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-04-11T22:36:39Z |
| publishDate | 2022-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-59d0c85ce45b4387879dd48fffc4f0a92022-12-22T03:59:11ZengSpringerOpenRice1939-84251939-84332022-08-0115111210.1186/s12284-022-00589-xFlavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain HullsFantao Zhang0Limin Yang1Wenxue Huang2Xiangdong Luo3Jiankun Xie4Biaolin Hu5Yaling Chen6Laboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal UniversityLaboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal UniversityLaboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal UniversityLaboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal UniversityLaboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal UniversityRice Research Institute, Jiangxi Academy of Agricultural Sciences/National Engineering Laboratory for Rice (Nanchang)Laboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal UniversityAbstract Rice (Oryza sativa L.) grain hull color is an easily observable trait and regarded as a crucial morphological marker in rice breeding. Here, a purple gradient grain hull mutant (pg) was found from natural mutations of a straw-white grain hull rice variety IARI 6184B (Orzya sativa L. subsp. indica). The color of the mutant grain hulls changed from straw-white to pink, then purple, and finally brownish-yellow. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) identified 217 flavonoids, including 18 anthocyanins, among which cyanidin O-syringic acid had the highest concentration in pink (66.2 × 106) and purple (68.0 × 106) grain hulls. The relative contents of hesperetin O-malonyl-hexoside, apigenin derivatives, genistein derivatives, and kaempferol 3-O derivatives were consistently downregulated during pg grain hull development. Conversely, 12 anthocyanins were upregulated in colored hulls, and cyanidin 3-O-malonylhexoside was abundant only in pink and purple grain hulls. Moreover, the candidate gene was mapped into a 1.38 Mb region on chromosome 4 through bulked segregant analysis based on deep sequencing (BSA-seq) and gene mapping approaches. These results increased our understanding of anthocyanin biosynthesis in rice grains, helping rice breeders to select new rice varieties with desirable grain traits.https://doi.org/10.1186/s12284-022-00589-xRicePurple gradient grain hullCyanidinBSA-seqFlavonoids |
| spellingShingle | Fantao Zhang Limin Yang Wenxue Huang Xiangdong Luo Jiankun Xie Biaolin Hu Yaling Chen Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls Rice Rice Purple gradient grain hull Cyanidin BSA-seq Flavonoids |
| title | Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls |
| title_full | Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls |
| title_fullStr | Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls |
| title_full_unstemmed | Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls |
| title_short | Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls |
| title_sort | flavonoid metabolic profiles and gene mapping of rice oryza sativa l purple gradient grain hulls |
| topic | Rice Purple gradient grain hull Cyanidin BSA-seq Flavonoids |
| url | https://doi.org/10.1186/s12284-022-00589-x |
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