In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds
Hydroxyapatite-collagen (HA/Col) composites are potential scaffolds for bone tissue engineering. In this work, three-dimensional (3-D) HA/Col (50/50 wt. (%)) scaffolds were synthesized using a self-assembly method and cross-linked with a 0.125% glutaraldehyde solution. Scaffolds were evaluated in vi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2012-02-01
|
| Series: | Materials Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392012000100021 |
| _version_ | 1819264114591006720 |
|---|---|
| author | Doris Moura Campos Karine Anselme Glória Dulce de Almeida Soares |
| author_facet | Doris Moura Campos Karine Anselme Glória Dulce de Almeida Soares |
| author_sort | Doris Moura Campos |
| collection | DOAJ |
| description | Hydroxyapatite-collagen (HA/Col) composites are potential scaffolds for bone tissue engineering. In this work, three-dimensional (3-D) HA/Col (50/50 wt. (%)) scaffolds were synthesized using a self-assembly method and cross-linked with a 0.125% glutaraldehyde solution. Scaffolds were evaluated in vitro by cytotoxicity testing using MC3T3 cells; proliferation and differentiation were studied using STRO-1A human stromal cells for up to 21 days. Morphological and histological examinations showed a fibrous structure with a good distribution and homogeneous HA particles distribution. By thermogravimetric analysis, a ratio of 1.2 between inorganic and organic phase was found. The scaffolds presented no cytotoxicity when evaluated using three different parameters of cell survival and integrity: 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl] -2H-tetrazolium-5-carboxanilide (XTT), Neutral Red (NR) and Crystal Violet Dye Elution (CVDE). STRO-1A cells were found to adhere, proliferate and differentiate on the 3-D scaffold, but limited cell penetration was observed. |
| first_indexed | 2024-12-23T20:24:20Z |
| format | Article |
| id | doaj.art-59d2feece0d14f2aa9a0e3623da0b0b2 |
| institution | Directory Open Access Journal |
| issn | 1516-1439 |
| language | English |
| last_indexed | 2024-12-23T20:24:20Z |
| publishDate | 2012-02-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj.art-59d2feece0d14f2aa9a0e3623da0b0b22022-12-21T17:32:26ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392012-02-01151151158In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) ScaffoldsDoris Moura CamposKarine AnselmeGlória Dulce de Almeida SoaresHydroxyapatite-collagen (HA/Col) composites are potential scaffolds for bone tissue engineering. In this work, three-dimensional (3-D) HA/Col (50/50 wt. (%)) scaffolds were synthesized using a self-assembly method and cross-linked with a 0.125% glutaraldehyde solution. Scaffolds were evaluated in vitro by cytotoxicity testing using MC3T3 cells; proliferation and differentiation were studied using STRO-1A human stromal cells for up to 21 days. Morphological and histological examinations showed a fibrous structure with a good distribution and homogeneous HA particles distribution. By thermogravimetric analysis, a ratio of 1.2 between inorganic and organic phase was found. The scaffolds presented no cytotoxicity when evaluated using three different parameters of cell survival and integrity: 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl] -2H-tetrazolium-5-carboxanilide (XTT), Neutral Red (NR) and Crystal Violet Dye Elution (CVDE). STRO-1A cells were found to adhere, proliferate and differentiate on the 3-D scaffold, but limited cell penetration was observed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392012000100021biomaterialstissues engineeringhydroxyapatitecollagencell culturescaffolds |
| spellingShingle | Doris Moura Campos Karine Anselme Glória Dulce de Almeida Soares In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds Materials Research biomaterials tissues engineering hydroxyapatite collagen cell culture scaffolds |
| title | In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds |
| title_full | In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds |
| title_fullStr | In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds |
| title_full_unstemmed | In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds |
| title_short | In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds |
| title_sort | in vitro biological evaluation of 3 d hydroxyapatite collagen 50 50 wt scaffolds |
| topic | biomaterials tissues engineering hydroxyapatite collagen cell culture scaffolds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392012000100021 |
| work_keys_str_mv | AT dorismouracampos invitrobiologicalevaluationof3dhydroxyapatitecollagen5050wtscaffolds AT karineanselme invitrobiologicalevaluationof3dhydroxyapatitecollagen5050wtscaffolds AT gloriadulcedealmeidasoares invitrobiologicalevaluationof3dhydroxyapatitecollagen5050wtscaffolds |