Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy)
Domestic dogs (<i>Canis lupus familiaris</i>) used for wild boar (<i>Sus scrofa</i>) hunting may represent incidental hosts for several zoonotic pathogens. This investigation aimed to evaluate the presence of anti-<i>Leptospira</i> antibodies and the occurrence, a...
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MDPI AG
2021-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/1139 |
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| author | Giovanni Cilia Filippo Fratini Barbara Turchi Valentina Virginia Ebani Luca Turini Stefano Bilei Teresa Bossù Maria Laura De Marchis Domenico Cerri Fabrizio Bertelloni |
| author_facet | Giovanni Cilia Filippo Fratini Barbara Turchi Valentina Virginia Ebani Luca Turini Stefano Bilei Teresa Bossù Maria Laura De Marchis Domenico Cerri Fabrizio Bertelloni |
| author_sort | Giovanni Cilia |
| collection | DOAJ |
| description | Domestic dogs (<i>Canis lupus familiaris</i>) used for wild boar (<i>Sus scrofa</i>) hunting may represent incidental hosts for several zoonotic pathogens. This investigation aimed to evaluate the presence of anti-<i>Leptospira</i> antibodies and the occurrence, antimicrobial resistance, and virulence of <i>Salmonella</i> spp., <i>Yersinia enterocolitica</i>, and <i>Listeria monocytogenes</i> in sera and rectal swabs collected from 42 domestic hunting dogs in the Tuscany region (Italy). Regarding <i>Leptospira</i>, 31 out of 42 serum samples (73.8%) were positive and serogroup Pomona was the most detected (71.4%) at titers between 1:100 and 1:400. Four <i>Salmonella</i> isolates (9.52%) were obtained, all belonging to serotype Infantis; two of them showed antimicrobial resistance to streptomycin, while <i>pipB</i> and <i>sopE</i> presence was assessed in all but one isolate. Concerning <i>Yersinia enterocolitica</i>, seven isolates (16.7%) were obtained, six belonging to biotype 1 and one to biotype 4. Resistance to amoxicillin–clavulanic acid, cephalothin, and ampicillin was detected. Biotype 4 presented three of the virulence genes searched (<i>ystA</i>, <i>ystB</i>, <i>inv</i>), while isolates of biotype 1 showed only one gene. No <i>Listeria monocytogenes</i> was isolated from dog rectal swabs. The results suggest that hunting dogs are exposed to different bacterial zoonotic agents, potentially linked to their work activity, and highlight the possible health risks for humans. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:16:20Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-59d6209812ea476099a53aa29c01e5ae2023-11-21T15:49:49ZengMDPI AGAnimals2076-26152021-04-01114113910.3390/ani11041139Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy)Giovanni Cilia0Filippo Fratini1Barbara Turchi2Valentina Virginia Ebani3Luca Turini4Stefano Bilei5Teresa Bossù6Maria Laura De Marchis7Domenico Cerri8Fabrizio Bertelloni9Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Rome, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDomestic dogs (<i>Canis lupus familiaris</i>) used for wild boar (<i>Sus scrofa</i>) hunting may represent incidental hosts for several zoonotic pathogens. This investigation aimed to evaluate the presence of anti-<i>Leptospira</i> antibodies and the occurrence, antimicrobial resistance, and virulence of <i>Salmonella</i> spp., <i>Yersinia enterocolitica</i>, and <i>Listeria monocytogenes</i> in sera and rectal swabs collected from 42 domestic hunting dogs in the Tuscany region (Italy). Regarding <i>Leptospira</i>, 31 out of 42 serum samples (73.8%) were positive and serogroup Pomona was the most detected (71.4%) at titers between 1:100 and 1:400. Four <i>Salmonella</i> isolates (9.52%) were obtained, all belonging to serotype Infantis; two of them showed antimicrobial resistance to streptomycin, while <i>pipB</i> and <i>sopE</i> presence was assessed in all but one isolate. Concerning <i>Yersinia enterocolitica</i>, seven isolates (16.7%) were obtained, six belonging to biotype 1 and one to biotype 4. Resistance to amoxicillin–clavulanic acid, cephalothin, and ampicillin was detected. Biotype 4 presented three of the virulence genes searched (<i>ystA</i>, <i>ystB</i>, <i>inv</i>), while isolates of biotype 1 showed only one gene. No <i>Listeria monocytogenes</i> was isolated from dog rectal swabs. The results suggest that hunting dogs are exposed to different bacterial zoonotic agents, potentially linked to their work activity, and highlight the possible health risks for humans.https://www.mdpi.com/2076-2615/11/4/1139<i>Leptospira</i><i>Salmonella</i><i>Yersinia</i><i>Listeria</i>zoonoseshunting dogs |
| spellingShingle | Giovanni Cilia Filippo Fratini Barbara Turchi Valentina Virginia Ebani Luca Turini Stefano Bilei Teresa Bossù Maria Laura De Marchis Domenico Cerri Fabrizio Bertelloni Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy) Animals <i>Leptospira</i> <i>Salmonella</i> <i>Yersinia</i> <i>Listeria</i> zoonoses hunting dogs |
| title | Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy) |
| title_full | Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy) |
| title_fullStr | Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy) |
| title_full_unstemmed | Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy) |
| title_short | Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (<i>Canis lupus familiaris</i>) in Tuscany (Italy) |
| title_sort | presence and characterization of zoonotic bacterial pathogens in wild boar hunting dogs i canis lupus familiaris i in tuscany italy |
| topic | <i>Leptospira</i> <i>Salmonella</i> <i>Yersinia</i> <i>Listeria</i> zoonoses hunting dogs |
| url | https://www.mdpi.com/2076-2615/11/4/1139 |
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