Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
Abstract Biosurfactants are amphiphilic surface-active molecules of microbial origin principally produced by hydrocarbon-degrading bacteria; in addition to the bioremediation properties, they can also present antimicrobial activity. The present study highlights the chemical characterization and the...
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| Format: | Article |
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Nature Portfolio
2021-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-95788-9 |
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| author | Albert D. Patiño Manuela Montoya-Giraldo Marynes Quintero Lizbeth L. López-Parra Lina M. Blandón Javier Gómez-León |
| author_facet | Albert D. Patiño Manuela Montoya-Giraldo Marynes Quintero Lizbeth L. López-Parra Lina M. Blandón Javier Gómez-León |
| author_sort | Albert D. Patiño |
| collection | DOAJ |
| description | Abstract Biosurfactants are amphiphilic surface-active molecules of microbial origin principally produced by hydrocarbon-degrading bacteria; in addition to the bioremediation properties, they can also present antimicrobial activity. The present study highlights the chemical characterization and the antimicrobial activities of biosurfactants produced by deep-sea marine bacteria from the genera Halomonas, Bacillus, Streptomyces, and Pseudomonas. The biosurfactants were extracted and chemically characterized through Chromatography TLC, FT-IR, LC/ESI–MS/MS, and a metabolic analysis was done through molecular networking. Six biosurfactants were identified by dereplication tools from GNPS and some surfactin isoforms were identified by molecular networking. The half-maximal inhibitory concentration (IC50) of biosurfactant from Halomonas sp. INV PRT125 (7.27 mg L−1) and Halomonas sp. INV PRT124 (8.92 mg L−1) were most effective against the pathogenic yeast Candida albicans ATCC 10231. For Methicillin-resistant Staphylococcus aureus ATCC 43300, the biosurfactant from Bacillus sp. INV FIR48 was the most effective with IC50 values of 25.65 mg L−1 and 21.54 mg L−1 for C. albicans, without hemolytic effect (< 1%), and non-ecotoxic effect in brine shrimp larvae (Artemia franciscana), with values under 150 mg L−1, being a biosurfactant promising for further study. The extreme environments as deep-sea can be an important source for the isolation of new biosurfactants-producing microorganisms with environmental and pharmaceutical use. |
| first_indexed | 2024-12-13T17:16:52Z |
| format | Article |
| id | doaj.art-59d6c9e4ca76453689eebb0819152418 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T17:16:52Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-59d6c9e4ca76453689eebb08191524182022-12-21T23:37:25ZengNature PortfolioScientific Reports2045-23222021-08-0111111210.1038/s41598-021-95788-9Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networkingAlbert D. Patiño0Manuela Montoya-Giraldo1Marynes Quintero2Lizbeth L. López-Parra3Lina M. Blandón4Javier Gómez-León5Marine Bioprospecting Line, Marine and Coastal Research Institute “José Benito Vives de Andréis”-INVEMARMarine Bioprospecting Line, Marine and Coastal Research Institute “José Benito Vives de Andréis”-INVEMARMarine Bioprospecting Line, Marine and Coastal Research Institute “José Benito Vives de Andréis”-INVEMARGrupo de Investigación en Electroquímica y Medio Ambiente (GIEMA), Universidad Santiago de CaliMarine Bioprospecting Line, Marine and Coastal Research Institute “José Benito Vives de Andréis”-INVEMARMarine Bioprospecting Line, Marine and Coastal Research Institute “José Benito Vives de Andréis”-INVEMARAbstract Biosurfactants are amphiphilic surface-active molecules of microbial origin principally produced by hydrocarbon-degrading bacteria; in addition to the bioremediation properties, they can also present antimicrobial activity. The present study highlights the chemical characterization and the antimicrobial activities of biosurfactants produced by deep-sea marine bacteria from the genera Halomonas, Bacillus, Streptomyces, and Pseudomonas. The biosurfactants were extracted and chemically characterized through Chromatography TLC, FT-IR, LC/ESI–MS/MS, and a metabolic analysis was done through molecular networking. Six biosurfactants were identified by dereplication tools from GNPS and some surfactin isoforms were identified by molecular networking. The half-maximal inhibitory concentration (IC50) of biosurfactant from Halomonas sp. INV PRT125 (7.27 mg L−1) and Halomonas sp. INV PRT124 (8.92 mg L−1) were most effective against the pathogenic yeast Candida albicans ATCC 10231. For Methicillin-resistant Staphylococcus aureus ATCC 43300, the biosurfactant from Bacillus sp. INV FIR48 was the most effective with IC50 values of 25.65 mg L−1 and 21.54 mg L−1 for C. albicans, without hemolytic effect (< 1%), and non-ecotoxic effect in brine shrimp larvae (Artemia franciscana), with values under 150 mg L−1, being a biosurfactant promising for further study. The extreme environments as deep-sea can be an important source for the isolation of new biosurfactants-producing microorganisms with environmental and pharmaceutical use.https://doi.org/10.1038/s41598-021-95788-9 |
| spellingShingle | Albert D. Patiño Manuela Montoya-Giraldo Marynes Quintero Lizbeth L. López-Parra Lina M. Blandón Javier Gómez-León Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking Scientific Reports |
| title | Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking |
| title_full | Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking |
| title_fullStr | Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking |
| title_full_unstemmed | Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking |
| title_short | Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking |
| title_sort | dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking |
| url | https://doi.org/10.1038/s41598-021-95788-9 |
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