First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report
Abstract: (206 words): Purpose: We report the use of a rapid multiplex polymerase chain reaction (PCR) system in the microbiological diagnosis and the therapeutic management of a severe bacterial keratitis case. Observations: During the management of a severe bacterial keratitis case, standard micr...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | American Journal of Ophthalmology Case Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2451993622003474 |
| _version_ | 1798036046335705088 |
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| author | Thomas Ferreira de Moura Anne Limelette Carl Arndt Thomas Guillard Laurent Andreoletti Alexandre Denoyer |
| author_facet | Thomas Ferreira de Moura Anne Limelette Carl Arndt Thomas Guillard Laurent Andreoletti Alexandre Denoyer |
| author_sort | Thomas Ferreira de Moura |
| collection | DOAJ |
| description | Abstract: (206 words): Purpose: We report the use of a rapid multiplex polymerase chain reaction (PCR) system in the microbiological diagnosis and the therapeutic management of a severe bacterial keratitis case. Observations: During the management of a severe bacterial keratitis case, standard microbiological diagnostic methods were performed. At the same time, an additional ocular swab sampling from the cornea was performed and analyzed using two rapid multiplex PCR assays allowing the simultaneous detection of 29 different virus, yeast and bacteria genomes. Using combination of two rapid multiplex PCR systems, the microbiological diagnosis of a severe Pseudomonas aeruginosa induced keratitis was performed within 90 minutes after an ocular sampling. A rapid subsequent adaptation of local antibiotic treatment was performed allowing to the young patient to regain 6 months after her hospital admission a final visual acuity of 20/20 in her right eye. Conclusions and importance: The present case report suggests that the use of a rapid multiplex PCR strategy may result in a decrease of the mean hospital stage duration for severe infectious keratitis and in an improvement of the clinical outcome of severe keratitis infections. Nevertheless, additional prospective studies are needed to evaluate whether this innovative strategy may replace the current standard approach and optimize the therapeutic management of severe corneal infections. |
| first_indexed | 2024-04-11T21:05:47Z |
| format | Article |
| id | doaj.art-59d8d89e48ad4242be7e12100a68bcc0 |
| institution | Directory Open Access Journal |
| issn | 2451-9936 |
| language | English |
| last_indexed | 2024-04-11T21:05:47Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | American Journal of Ophthalmology Case Reports |
| spelling | doaj.art-59d8d89e48ad4242be7e12100a68bcc02022-12-22T04:03:16ZengElsevierAmerican Journal of Ophthalmology Case Reports2451-99362022-09-0127101601First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case reportThomas Ferreira de Moura0Anne Limelette1Carl Arndt2Thomas Guillard3Laurent Andreoletti4Alexandre Denoyer5University of Reims Champagne-Ardenne, University Hospital, Ophthalmology, Reims, France; University of Reims Champagne-Ardenne, Research Team EA4684, CARDIOVIR, Reims, FranceUniversity Hospital, Department of Biology, Reims, FranceUniversity of Reims Champagne-Ardenne, University Hospital, Ophthalmology, Reims, FranceUniversity Hospital, Department of Biology, Reims, France; University of Reims Champagne-Ardenne, Inserm UMRS1250, P3Cell, Reims, FranceUniversity of Reims Champagne-Ardenne, Research Team EA4684, CARDIOVIR, Reims, France; University Hospital, Department of Biology, Reims, FranceUniversity of Reims Champagne-Ardenne, University Hospital, Ophthalmology, Reims, France; University of Reims Champagne-Ardenne, Research Team EA4684, CARDIOVIR, Reims, France; Corresponding author. Department of ophthalmology, Robert Debré University Hospital, rue du general Koenig, 51100, Reims, France.Abstract: (206 words): Purpose: We report the use of a rapid multiplex polymerase chain reaction (PCR) system in the microbiological diagnosis and the therapeutic management of a severe bacterial keratitis case. Observations: During the management of a severe bacterial keratitis case, standard microbiological diagnostic methods were performed. At the same time, an additional ocular swab sampling from the cornea was performed and analyzed using two rapid multiplex PCR assays allowing the simultaneous detection of 29 different virus, yeast and bacteria genomes. Using combination of two rapid multiplex PCR systems, the microbiological diagnosis of a severe Pseudomonas aeruginosa induced keratitis was performed within 90 minutes after an ocular sampling. A rapid subsequent adaptation of local antibiotic treatment was performed allowing to the young patient to regain 6 months after her hospital admission a final visual acuity of 20/20 in her right eye. Conclusions and importance: The present case report suggests that the use of a rapid multiplex PCR strategy may result in a decrease of the mean hospital stage duration for severe infectious keratitis and in an improvement of the clinical outcome of severe keratitis infections. Nevertheless, additional prospective studies are needed to evaluate whether this innovative strategy may replace the current standard approach and optimize the therapeutic management of severe corneal infections.http://www.sciencedirect.com/science/article/pii/S2451993622003474Fortified antibioticCorneaInfectious keratitisRapid multiplex system |
| spellingShingle | Thomas Ferreira de Moura Anne Limelette Carl Arndt Thomas Guillard Laurent Andreoletti Alexandre Denoyer First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report American Journal of Ophthalmology Case Reports Fortified antibiotic Cornea Infectious keratitis Rapid multiplex system |
| title | First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report |
| title_full | First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report |
| title_fullStr | First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report |
| title_full_unstemmed | First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report |
| title_short | First use of a new rapid multiplex PCR system for the microbiological diagnosis and the clinical management of severe infectious keratitis: A case report |
| title_sort | first use of a new rapid multiplex pcr system for the microbiological diagnosis and the clinical management of severe infectious keratitis a case report |
| topic | Fortified antibiotic Cornea Infectious keratitis Rapid multiplex system |
| url | http://www.sciencedirect.com/science/article/pii/S2451993622003474 |
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