Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.)
Clubroot is caused by Plasmodiophora brassicae, which threatens Brassicaceae crop production worldwide. In recent years, there has been an outbreak and rapid spread of clubroot in many major cruciferous crop-producing areas of China. In this study, we identified a cabbage material DingWen (DW) with...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.898108/full |
| _version_ | 1817992927496896512 |
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| author | Yanyan Wang Xianyu Xiang Fan Huang Wenlin Yu Xueqing Zhou Baojun Li Baojun Li Yunyun Zhang Yunyun Zhang Peng Chen Chunyu Zhang |
| author_facet | Yanyan Wang Xianyu Xiang Fan Huang Wenlin Yu Xueqing Zhou Baojun Li Baojun Li Yunyun Zhang Yunyun Zhang Peng Chen Chunyu Zhang |
| author_sort | Yanyan Wang |
| collection | DOAJ |
| description | Clubroot is caused by Plasmodiophora brassicae, which threatens Brassicaceae crop production worldwide. In recent years, there has been an outbreak and rapid spread of clubroot in many major cruciferous crop-producing areas of China. In this study, we identified a cabbage material DingWen (DW) with different resistant capabilities from Huashuang5R (H5R) and Huayouza62R of Brassica napus, which are currently used as the main resistant cultivars for clubroot management in China. We used a next-generation sequencing-based bulked segregant analysis approach, combined with genetic mapping to identify clubroot-resistant (CR) genes from F1 population generated from a cross between the DW (CR) and HZSX (clubroot susceptible). The CR locus of DW (named CRA8.1) was mapped to a region between markers A08-4346 and A08-4853, which contains two different loci CRA8.1a and CRA8.1b after fine mapping. The CRA8.1b loci contain a fragment of 395 kb between markers A08-4624 and A08-4853 on A08 chromosome, and it is responsible for the resistance to PbZj and PbXm isolates. However, together with CRA8.1a, corresponding to a 765-kb region between markers A08-4346 and A08-4624, then it can confer resistance to PbXm+. Finally, through expression analysis between resistant and susceptible materials, two genes encoding TIR-NBS-LRR proteins (BraA08g039211E and BraA08g039212E) and one gene encoding an RLP protein (BraA08g039193E) were identified to be the most likely CR candidates for the peculiar resistance in DW. |
| first_indexed | 2024-04-14T01:32:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T01:32:44Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-59db44541ad74195b2b30b0b3c1707ba2022-12-22T02:20:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.898108898108Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.)Yanyan Wang0Xianyu Xiang1Fan Huang2Wenlin Yu3Xueqing Zhou4Baojun Li5Baojun Li6Yunyun Zhang7Yunyun Zhang8Peng Chen9Chunyu Zhang10College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaHybrid Rape Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement, Yangling, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaIndustrial Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaClubroot is caused by Plasmodiophora brassicae, which threatens Brassicaceae crop production worldwide. In recent years, there has been an outbreak and rapid spread of clubroot in many major cruciferous crop-producing areas of China. In this study, we identified a cabbage material DingWen (DW) with different resistant capabilities from Huashuang5R (H5R) and Huayouza62R of Brassica napus, which are currently used as the main resistant cultivars for clubroot management in China. We used a next-generation sequencing-based bulked segregant analysis approach, combined with genetic mapping to identify clubroot-resistant (CR) genes from F1 population generated from a cross between the DW (CR) and HZSX (clubroot susceptible). The CR locus of DW (named CRA8.1) was mapped to a region between markers A08-4346 and A08-4853, which contains two different loci CRA8.1a and CRA8.1b after fine mapping. The CRA8.1b loci contain a fragment of 395 kb between markers A08-4624 and A08-4853 on A08 chromosome, and it is responsible for the resistance to PbZj and PbXm isolates. However, together with CRA8.1a, corresponding to a 765-kb region between markers A08-4346 and A08-4624, then it can confer resistance to PbXm+. Finally, through expression analysis between resistant and susceptible materials, two genes encoding TIR-NBS-LRR proteins (BraA08g039211E and BraA08g039212E) and one gene encoding an RLP protein (BraA08g039193E) were identified to be the most likely CR candidates for the peculiar resistance in DW.https://www.frontiersin.org/articles/10.3389/fpls.2022.898108/fullclubrootPlasmodiophora brassicaeBrassica rapaCRA8.1fine mapping |
| spellingShingle | Yanyan Wang Xianyu Xiang Fan Huang Wenlin Yu Xueqing Zhou Baojun Li Baojun Li Yunyun Zhang Yunyun Zhang Peng Chen Chunyu Zhang Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.) Frontiers in Plant Science clubroot Plasmodiophora brassicae Brassica rapa CRA8.1 fine mapping |
| title | Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.) |
| title_full | Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.) |
| title_fullStr | Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.) |
| title_full_unstemmed | Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.) |
| title_short | Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.) |
| title_sort | fine mapping of clubroot resistance loci cra8 1 and candidate gene analysis in chinese cabbage brassica rapa l |
| topic | clubroot Plasmodiophora brassicae Brassica rapa CRA8.1 fine mapping |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.898108/full |
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