The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Incubation of rat hepatocytes with glucagon results in a time- and dose-dependent decrease in the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase. We demonstrate, using immunoprecipitation of radiolabeled enzyme, that 10 nM glucagon inhibits the synthesis of the enzyme by approximately 5...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1988-06-01
|
| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520388210 |
| _version_ | 1830372433004068864 |
|---|---|
| author | P A Edwards S F Lan A M Fogelman |
| author_facet | P A Edwards S F Lan A M Fogelman |
| author_sort | P A Edwards |
| collection | DOAJ |
| description | Incubation of rat hepatocytes with glucagon results in a time- and dose-dependent decrease in the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase. We demonstrate, using immunoprecipitation of radiolabeled enzyme, that 10 nM glucagon inhibits the synthesis of the enzyme by approximately 50%, but that the apparent rate of degradation of the enzyme is not affected by the hormone. We also demonstrate that the intact reductase polypeptide contained phosphoserine. We conclude that glucagon inhibits the activity of the reductase by inhibition of enzyme synthesis. |
| first_indexed | 2024-12-20T07:02:44Z |
| format | Article |
| id | doaj.art-59dc66c29654482fbca83560ff6920bf |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T07:02:44Z |
| publishDate | 1988-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-59dc66c29654482fbca83560ff6920bf2022-12-21T19:49:10ZengElsevierJournal of Lipid Research0022-22751988-06-01274398403The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.P A EdwardsS F LanA M FogelmanIncubation of rat hepatocytes with glucagon results in a time- and dose-dependent decrease in the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase. We demonstrate, using immunoprecipitation of radiolabeled enzyme, that 10 nM glucagon inhibits the synthesis of the enzyme by approximately 50%, but that the apparent rate of degradation of the enzyme is not affected by the hormone. We also demonstrate that the intact reductase polypeptide contained phosphoserine. We conclude that glucagon inhibits the activity of the reductase by inhibition of enzyme synthesis.http://www.sciencedirect.com/science/article/pii/S0022227520388210 |
| spellingShingle | P A Edwards S F Lan A M Fogelman The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Journal of Lipid Research |
| title | The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| title_full | The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| title_fullStr | The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| title_full_unstemmed | The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| title_short | The effect of glucagon on the synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. |
| title_sort | effect of glucagon on the synthesis and degradation of 3 hydroxy 3 methylglutaryl coenzyme a reductase |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520388210 |
| work_keys_str_mv | AT paedwards theeffectofglucagononthesynthesisanddegradationof3hydroxy3methylglutarylcoenzymeareductase AT sflan theeffectofglucagononthesynthesisanddegradationof3hydroxy3methylglutarylcoenzymeareductase AT amfogelman theeffectofglucagononthesynthesisanddegradationof3hydroxy3methylglutarylcoenzymeareductase AT paedwards effectofglucagononthesynthesisanddegradationof3hydroxy3methylglutarylcoenzymeareductase AT sflan effectofglucagononthesynthesisanddegradationof3hydroxy3methylglutarylcoenzymeareductase AT amfogelman effectofglucagononthesynthesisanddegradationof3hydroxy3methylglutarylcoenzymeareductase |