Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
Osteoarthritis (OA) is the most prevalent rheumatic disease in the world. Although its etiology is still unknown, one of the key processes in OA progression and development is oxidative stress. In this context, resveratrol, a well-known anti-oxidant from the stilbene family, could be of particular i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.00538/full |
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| author | Solenn Le Clanche Solenn Le Clanche Tristan Cheminel François Rannou François Rannou Dominique Bonnefont-Rousselot Dominique Bonnefont-Rousselot Dominique Bonnefont-Rousselot Didier Borderie Didier Borderie Didier Borderie Christine Charrueau |
| author_facet | Solenn Le Clanche Solenn Le Clanche Tristan Cheminel François Rannou François Rannou Dominique Bonnefont-Rousselot Dominique Bonnefont-Rousselot Dominique Bonnefont-Rousselot Didier Borderie Didier Borderie Didier Borderie Christine Charrueau |
| author_sort | Solenn Le Clanche |
| collection | DOAJ |
| description | Osteoarthritis (OA) is the most prevalent rheumatic disease in the world. Although its etiology is still unknown, one of the key processes in OA progression and development is oxidative stress. In this context, resveratrol, a well-known anti-oxidant from the stilbene family, could be of particular interest in future OA therapeutic strategies. However, currently, because of its low bioavailability, use of resveratrol in human health is very limited. In this study, we tested two resveratrol self-emulsifying systems previously developed in our laboratory in order to determine if they could improve cellular uptake of resveratrol in a human immortalized chondrocytic cell line (T/C28a2) and enhance protection against oxidative stress. Our results showed that resveratrol self-emulsifying systems were able first to increase cellular tolerance towards resveratrol, and thus decrease resveratrol intrinsic cellular toxicity, allowing the use of higher concentrations, second, to increase resveratrol uptake in membrane and intracellular fractions, and finally, to improve protection against oxidative stress-mediated death in human immortalized chondrocytic cell line T/C28a2. These data suggest that new formulations of resveratrol could be considered as potential beneficial effectors in future OA treatments. |
| first_indexed | 2024-04-13T09:30:04Z |
| format | Article |
| id | doaj.art-59ddc00afae74a9eb4c8c219b55c8b30 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T09:30:04Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-59ddc00afae74a9eb4c8c219b55c8b302022-12-22T02:52:18ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-05-01910.3389/fphar.2018.00538369103Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated DeathSolenn Le Clanche0Solenn Le Clanche1Tristan Cheminel2François Rannou3François Rannou4Dominique Bonnefont-Rousselot5Dominique Bonnefont-Rousselot6Dominique Bonnefont-Rousselot7Didier Borderie8Didier Borderie9Didier Borderie10Christine Charrueau11UMR-S 1124 INSERM Toxicologie, Pharmacologie et Signalisation Cellulaire, CUSP, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceUnité Pédagogique de Biochimie, Faculté de Pharmacie de Paris, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceUnité Pédagogique de Biochimie, Faculté de Pharmacie de Paris, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceUMR-S 1124 INSERM Toxicologie, Pharmacologie et Signalisation Cellulaire, CUSP, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceService de Rééducation et de Réadaptation de l’Appareil Locomoteur et des Pathologies du Rachis, Hôpital Cochin (AP-HP), Paris, FranceUnité Pédagogique de Biochimie, Faculté de Pharmacie de Paris, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceService de Biochimie Métabolique, Hôpitaux Universitaires Pitié-Salpêtrière-Charles Foix (AP-HP), Paris, FranceINSERM U 1022 CNRS UMR 8258, Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Faculté de Pharmacie de Paris, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceUMR-S 1124 INSERM Toxicologie, Pharmacologie et Signalisation Cellulaire, CUSP, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceUnité Pédagogique de Biochimie, Faculté de Pharmacie de Paris, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceService de Diagnostic Biologique Automatisé, Hôpital Cochin (AP-HP), Paris, FranceINSERM U 1022 CNRS UMR 8258, Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Faculté de Pharmacie de Paris, Sorbonne Paris Cité, Université Paris Descartes, Paris, FranceOsteoarthritis (OA) is the most prevalent rheumatic disease in the world. Although its etiology is still unknown, one of the key processes in OA progression and development is oxidative stress. In this context, resveratrol, a well-known anti-oxidant from the stilbene family, could be of particular interest in future OA therapeutic strategies. However, currently, because of its low bioavailability, use of resveratrol in human health is very limited. In this study, we tested two resveratrol self-emulsifying systems previously developed in our laboratory in order to determine if they could improve cellular uptake of resveratrol in a human immortalized chondrocytic cell line (T/C28a2) and enhance protection against oxidative stress. Our results showed that resveratrol self-emulsifying systems were able first to increase cellular tolerance towards resveratrol, and thus decrease resveratrol intrinsic cellular toxicity, allowing the use of higher concentrations, second, to increase resveratrol uptake in membrane and intracellular fractions, and finally, to improve protection against oxidative stress-mediated death in human immortalized chondrocytic cell line T/C28a2. These data suggest that new formulations of resveratrol could be considered as potential beneficial effectors in future OA treatments.https://www.frontiersin.org/article/10.3389/fphar.2018.00538/fullanti-oxidative activitychondrocyteosteoarthritisnano-emulsionresveratrol |
| spellingShingle | Solenn Le Clanche Solenn Le Clanche Tristan Cheminel François Rannou François Rannou Dominique Bonnefont-Rousselot Dominique Bonnefont-Rousselot Dominique Bonnefont-Rousselot Didier Borderie Didier Borderie Didier Borderie Christine Charrueau Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death Frontiers in Pharmacology anti-oxidative activity chondrocyte osteoarthritis nano-emulsion resveratrol |
| title | Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death |
| title_full | Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death |
| title_fullStr | Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death |
| title_full_unstemmed | Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death |
| title_short | Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death |
| title_sort | use of resveratrol self emulsifying systems in t c28a2 cell line as beneficial effectors in cellular uptake and protection against oxidative stress mediated death |
| topic | anti-oxidative activity chondrocyte osteoarthritis nano-emulsion resveratrol |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.00538/full |
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