Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN
Although miR-193a-3p has been found to be dysregulated in variety of human tumors, little is known about its role in renal cell carcinoma. This study was designed to investigate the function and underlying mechanism of miR-193a-3p in human renal cell carcinoma tissues and cell lines. Here, we demons...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IOS Press
2017-06-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317711951 |
| _version_ | 1818573589263155200 |
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| author | Lingqi Liu Yanqin Li Shuchao Liu Qixin Duan Liang Chen Tianpeng Wu Huijun Qian Sixing Yang Dianqi Xin |
| author_facet | Lingqi Liu Yanqin Li Shuchao Liu Qixin Duan Liang Chen Tianpeng Wu Huijun Qian Sixing Yang Dianqi Xin |
| author_sort | Lingqi Liu |
| collection | DOAJ |
| description | Although miR-193a-3p has been found to be dysregulated in variety of human tumors, little is known about its role in renal cell carcinoma. This study was designed to investigate the function and underlying mechanism of miR-193a-3p in human renal cell carcinoma tissues and cell lines. Here, we demonstrated that the expression of miR-193-3p was increased in renal cell carcinoma tissues and cell lines. In addition, knockdown of miR-193a-3p significantly inhibited cell proliferation and colony formation and induced cells into G1 phase arrest. Meanwhile, the migration potential of 786-O cells was also decreased compared to control group. Furthermore, we identified PTEN as a direct and functional target of miR-193a-3p, at least partly responsible for promoting tumor effect of miR-193a-3p in renal cell carcinoma. Taken together, the findings indicated for the first time that miR-193a-3p functions as a tumor-promoting microRNA by directly targeting PTEN in renal cell carcinoma. |
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| format | Article |
| id | doaj.art-59def5e4f0c24dbdb7eac36740d865e1 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-15T00:13:22Z |
| publishDate | 2017-06-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-59def5e4f0c24dbdb7eac36740d865e12022-12-21T22:42:30ZengIOS PressTumor Biology1423-03802017-06-013910.1177/1010428317711951Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTENLingqi Liu0Yanqin Li1Shuchao Liu2Qixin Duan3Liang Chen4Tianpeng Wu5Huijun Qian6Sixing Yang7Dianqi Xin8Department of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaSchool of Pharmaceutical Sciences, Wuhan University, Wuhan, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Peking University First Hospital, Beijing, ChinaAlthough miR-193a-3p has been found to be dysregulated in variety of human tumors, little is known about its role in renal cell carcinoma. This study was designed to investigate the function and underlying mechanism of miR-193a-3p in human renal cell carcinoma tissues and cell lines. Here, we demonstrated that the expression of miR-193-3p was increased in renal cell carcinoma tissues and cell lines. In addition, knockdown of miR-193a-3p significantly inhibited cell proliferation and colony formation and induced cells into G1 phase arrest. Meanwhile, the migration potential of 786-O cells was also decreased compared to control group. Furthermore, we identified PTEN as a direct and functional target of miR-193a-3p, at least partly responsible for promoting tumor effect of miR-193a-3p in renal cell carcinoma. Taken together, the findings indicated for the first time that miR-193a-3p functions as a tumor-promoting microRNA by directly targeting PTEN in renal cell carcinoma.https://doi.org/10.1177/1010428317711951 |
| spellingShingle | Lingqi Liu Yanqin Li Shuchao Liu Qixin Duan Liang Chen Tianpeng Wu Huijun Qian Sixing Yang Dianqi Xin Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN Tumor Biology |
| title | Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN |
| title_full | Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN |
| title_fullStr | Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN |
| title_full_unstemmed | Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN |
| title_short | Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN |
| title_sort | downregulation of mir 193a 3p inhibits cell growth and migration in renal cell carcinoma by targeting pten |
| url | https://doi.org/10.1177/1010428317711951 |
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