3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene
Cell culturing methods in its classical 2D approach have limitations associated with altered cell morphology, gene expression patterns, migration, cell cycle and proliferation. Moreover, high throughput drug screening is mainly performed on 2D cell cultures which are physiologically far from proper...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/7/2087 |
| _version_ | 1827623061476605952 |
|---|---|
| author | Aleksey A. Ustyugov Nataliya A. Sipyagina Alena N. Malkova Elena A. Straumal Lyudmila L. Yurkova Anastasiya A. Globa Maria A. Lapshina Maria M. Chicheva Kirill D. Chaprov Aleksey V. Maksimkin Sergey A. Lermontov |
| author_facet | Aleksey A. Ustyugov Nataliya A. Sipyagina Alena N. Malkova Elena A. Straumal Lyudmila L. Yurkova Anastasiya A. Globa Maria A. Lapshina Maria M. Chicheva Kirill D. Chaprov Aleksey V. Maksimkin Sergey A. Lermontov |
| author_sort | Aleksey A. Ustyugov |
| collection | DOAJ |
| description | Cell culturing methods in its classical 2D approach have limitations associated with altered cell morphology, gene expression patterns, migration, cell cycle and proliferation. Moreover, high throughput drug screening is mainly performed on 2D cell cultures which are physiologically far from proper cell functions resulting in inadequate hit-compounds which subsequently fail. A shift to 3D culturing protocols could solve issues with altered cell biochemistry and signaling which would lead to a proper recapitulation of physiological conditions in test systems. Here, we examined porous ultra-high molecular weight polyethylene (UHMWPE) as an inexpensive and robust material with varying pore sizes for cell culturing. We tested and developed culturing protocols for immortalized human neuroblastoma and primary mice hippocampal cells which resulted in high rate of cell penetration within one week of cultivation. UHMWPE was additionally functionalized with gelatin, poly-L-lysine, BSA and chitosan, resulting in increased cell penetrations of the material. We have also successfully traced GFP-tagged cells which were grown on a UHMWPE sample after one week from implantation into mice brain. Our findings highlight the importance of UHMWPE use as a 3D matrix and show new possibilities arising from the use of cheap and chemically homogeneous material for studying various types of cell-surface interactions further improving cell adhesion, viability and biocompatibility. |
| first_indexed | 2024-03-09T11:36:56Z |
| format | Article |
| id | doaj.art-59e021c60d0f482283e758b4e9365eda |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:36:56Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-59e021c60d0f482283e758b4e9365eda2023-11-30T23:39:24ZengMDPI AGMolecules1420-30492022-03-01277208710.3390/molecules270720873D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight PolyethyleneAleksey A. Ustyugov0Nataliya A. Sipyagina1Alena N. Malkova2Elena A. Straumal3Lyudmila L. Yurkova4Anastasiya A. Globa5Maria A. Lapshina6Maria M. Chicheva7Kirill D. Chaprov8Aleksey V. Maksimkin9Sergey A. Lermontov10Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaInstitute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severnij proezd, 142432 Chernogolovka, RussiaCell culturing methods in its classical 2D approach have limitations associated with altered cell morphology, gene expression patterns, migration, cell cycle and proliferation. Moreover, high throughput drug screening is mainly performed on 2D cell cultures which are physiologically far from proper cell functions resulting in inadequate hit-compounds which subsequently fail. A shift to 3D culturing protocols could solve issues with altered cell biochemistry and signaling which would lead to a proper recapitulation of physiological conditions in test systems. Here, we examined porous ultra-high molecular weight polyethylene (UHMWPE) as an inexpensive and robust material with varying pore sizes for cell culturing. We tested and developed culturing protocols for immortalized human neuroblastoma and primary mice hippocampal cells which resulted in high rate of cell penetration within one week of cultivation. UHMWPE was additionally functionalized with gelatin, poly-L-lysine, BSA and chitosan, resulting in increased cell penetrations of the material. We have also successfully traced GFP-tagged cells which were grown on a UHMWPE sample after one week from implantation into mice brain. Our findings highlight the importance of UHMWPE use as a 3D matrix and show new possibilities arising from the use of cheap and chemically homogeneous material for studying various types of cell-surface interactions further improving cell adhesion, viability and biocompatibility.https://www.mdpi.com/1420-3049/27/7/2087UHMWPE3D cell culturescaffoldscell function |
| spellingShingle | Aleksey A. Ustyugov Nataliya A. Sipyagina Alena N. Malkova Elena A. Straumal Lyudmila L. Yurkova Anastasiya A. Globa Maria A. Lapshina Maria M. Chicheva Kirill D. Chaprov Aleksey V. Maksimkin Sergey A. Lermontov 3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene Molecules UHMWPE 3D cell culture scaffolds cell function |
| title | 3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene |
| title_full | 3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene |
| title_fullStr | 3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene |
| title_full_unstemmed | 3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene |
| title_short | 3D Neuronal Cell Culture Modeling Based on Highly Porous Ultra-High Molecular Weight Polyethylene |
| title_sort | 3d neuronal cell culture modeling based on highly porous ultra high molecular weight polyethylene |
| topic | UHMWPE 3D cell culture scaffolds cell function |
| url | https://www.mdpi.com/1420-3049/27/7/2087 |
| work_keys_str_mv | AT alekseyaustyugov 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT nataliyaasipyagina 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT alenanmalkova 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT elenaastraumal 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT lyudmilalyurkova 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT anastasiyaagloba 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT mariaalapshina 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT mariamchicheva 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT kirilldchaprov 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT alekseyvmaksimkin 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene AT sergeyalermontov 3dneuronalcellculturemodelingbasedonhighlyporousultrahighmolecularweightpolyethylene |