MicroRNA profiling of diabetic atherosclerosis in a rat model
Abstract Objective The incidence of diabetic atherosclerosis (DA) is increasing worldwide. The study aim was to identify differentially expressed microRNAs (DE-miRs) potentially associated with the initiation and/or progression of DA, thereby yielding new insights into this disease. Methods Matched...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40001-018-0354-5 |
| _version_ | 1819098695188086784 |
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| author | Yuejin Li Le Xiao Jinyuan Li Ping Sun Lei Shang Jian Zhang Quan Zhao Yiming Ouyang Linhai Li Kunmei Gong |
| author_facet | Yuejin Li Le Xiao Jinyuan Li Ping Sun Lei Shang Jian Zhang Quan Zhao Yiming Ouyang Linhai Li Kunmei Gong |
| author_sort | Yuejin Li |
| collection | DOAJ |
| description | Abstract Objective The incidence of diabetic atherosclerosis (DA) is increasing worldwide. The study aim was to identify differentially expressed microRNAs (DE-miRs) potentially associated with the initiation and/or progression of DA, thereby yielding new insights into this disease. Methods Matched iliac artery tissue samples were isolated from 6 male rats with or without DA. The Affymetrix GeneChip microRNA 4.0 Array was used to detect miRs. Differential expression between atherosclerotic group and non-atherosclerotic group samples was analyzed using the Gene-Cloud of Biotechnology Information platform. Targetscan and miRanda were then used to predict targets of DE-miRs. Functions and pathways were identified for significantly enriched candidate target genes and a DE-miR functional regulatory network was assembled to identify DA-associated core target genes. Results A total of nine DE-miRs (rno-miR-206-3p, rno-miR-133a-5p, rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-325-5p, rno-miR-675-3p, rno-miR-411-5p, rno-miR-329-3p, and rno-miR-126a-3p) were identified, all of which were up-regulated and together predicted to target 3349 genes. The target genes were enriched in known functions and pathways related to lipid and glucose metabolism. The functional regulatory network indicated a modulatory pattern of these metabolic functions with DE-miRs. The miR-gene network suggested arpp19 and MDM4 as possible DA-related core target genes. Conclusion The present study identified DE-miRs and miRNA-gene networks enriched for lipid and glucose metabolic functions and pathways, and arpp19 and MDM4 as potential DA-related core target genes, suggesting DE-miRs and/or arpp19 and MDM4 could act as potential diagnostic markers or therapeutic targets for DA. |
| first_indexed | 2024-12-22T00:35:04Z |
| format | Article |
| id | doaj.art-59e3b270c98840d2af253e8487e669fc |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-22T00:35:04Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-59e3b270c98840d2af253e8487e669fc2022-12-21T18:44:49ZengBMCEuropean Journal of Medical Research2047-783X2018-11-0123111010.1186/s40001-018-0354-5MicroRNA profiling of diabetic atherosclerosis in a rat modelYuejin Li0Le Xiao1Jinyuan Li2Ping Sun3Lei Shang4Jian Zhang5Quan Zhao6Yiming Ouyang7Linhai Li8Kunmei Gong9The First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceThe First Department of General Surgery, The First People’s Hospital of Yunnan ProvinceAbstract Objective The incidence of diabetic atherosclerosis (DA) is increasing worldwide. The study aim was to identify differentially expressed microRNAs (DE-miRs) potentially associated with the initiation and/or progression of DA, thereby yielding new insights into this disease. Methods Matched iliac artery tissue samples were isolated from 6 male rats with or without DA. The Affymetrix GeneChip microRNA 4.0 Array was used to detect miRs. Differential expression between atherosclerotic group and non-atherosclerotic group samples was analyzed using the Gene-Cloud of Biotechnology Information platform. Targetscan and miRanda were then used to predict targets of DE-miRs. Functions and pathways were identified for significantly enriched candidate target genes and a DE-miR functional regulatory network was assembled to identify DA-associated core target genes. Results A total of nine DE-miRs (rno-miR-206-3p, rno-miR-133a-5p, rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-325-5p, rno-miR-675-3p, rno-miR-411-5p, rno-miR-329-3p, and rno-miR-126a-3p) were identified, all of which were up-regulated and together predicted to target 3349 genes. The target genes were enriched in known functions and pathways related to lipid and glucose metabolism. The functional regulatory network indicated a modulatory pattern of these metabolic functions with DE-miRs. The miR-gene network suggested arpp19 and MDM4 as possible DA-related core target genes. Conclusion The present study identified DE-miRs and miRNA-gene networks enriched for lipid and glucose metabolic functions and pathways, and arpp19 and MDM4 as potential DA-related core target genes, suggesting DE-miRs and/or arpp19 and MDM4 could act as potential diagnostic markers or therapeutic targets for DA.http://link.springer.com/article/10.1186/s40001-018-0354-5Diabetic atherosclerosisDifferentially expressed miRNAmiRNA-gene regulatory network |
| spellingShingle | Yuejin Li Le Xiao Jinyuan Li Ping Sun Lei Shang Jian Zhang Quan Zhao Yiming Ouyang Linhai Li Kunmei Gong MicroRNA profiling of diabetic atherosclerosis in a rat model European Journal of Medical Research Diabetic atherosclerosis Differentially expressed miRNA miRNA-gene regulatory network |
| title | MicroRNA profiling of diabetic atherosclerosis in a rat model |
| title_full | MicroRNA profiling of diabetic atherosclerosis in a rat model |
| title_fullStr | MicroRNA profiling of diabetic atherosclerosis in a rat model |
| title_full_unstemmed | MicroRNA profiling of diabetic atherosclerosis in a rat model |
| title_short | MicroRNA profiling of diabetic atherosclerosis in a rat model |
| title_sort | microrna profiling of diabetic atherosclerosis in a rat model |
| topic | Diabetic atherosclerosis Differentially expressed miRNA miRNA-gene regulatory network |
| url | http://link.springer.com/article/10.1186/s40001-018-0354-5 |
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