| Summary: | Roghayeh Norouzi,1 Amin Ataei,1 Marzie Hejazy,2 Ayman Noreddin,3,4 Mohamed Ezzat El Zowalaty5 1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 2Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 3Infectious Diseases and Anti-Infective Therapy Research Group, Sharjah Medical Research Institute and College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates; 4Department of Medicine, School of Medicine, University of California Irvine, Irvine, CA, USA; 5Department of Pharmacy, City University College of Ajman, Ajman 18484, United Arab EmiratesCorrespondence: Roghayeh NorouziDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranEmail roghayehnorouzi123@gmail.comMohamed Ezzat El ZowalatyDepartment of Pharmacy, City University College of Ajman, Ajman 18484, United Arab EmiratesEmail elzow005@gmail.comBackground: Echinococcus granulosus is causative agent of cystic echinococcosis (CE), which has a cosmopolitan distribution. The current methods for the treatment of human CE include surgery. Therefore, the development of new scolicidal agents with low side effects and more efficacies is an urgent need.Purpose: The present study aimed to compare the scolicidal efficacies of silver, iron, copper, silica and zinc oxide nanoparticles (NPs) against hydatid cyst protoscolices in vitro.Methods: Hydatid cysts of sheep liver and lung were collected. The cyst fluid containing protoscolices was aspirated aseptically. The scolicidal activities of the silver, iron, copper, silica and zinc nanoparticles (Ag-NP, Fe-NP, Cu-NP, Si-NP and Zn-NP) were tested at different concentrations of 0.25, 0.5 and 1 mg/mL following 10, 30 and 60 min of incubation in triplicate. Viability of protoscolices was confirmed by 0.1% eosin staining.Results: Results showed that Ag-NPs at all concentrations tested had the highest scolicidal effect. Ag-NPs at 1 mg/mL concentration after 60 min of exposure time showed 80% mortality rate. Si-NPs had the high scolicidal activity at 1 mg/mL concentration (52.33%), Cu-NPs at 0.5 mg/mL concentration (41%), Fe-NPs at 1mg/mL concentration (28%) and Zn-NPs at concentration of 1mg/mL after 60 mins (15.67%).Conclusion: The findings of the present study showed that Ag-NPs, Fe-NPs, Cu-NPs, Si-NPs and Zn-NPs had potent scolicidal effects and that Ag-NPs are recommended as effective scolicidal agents. However, further in vivo studies are required to evaluate the efficacy of these nanoparticles.Keywords: scolicidal, hydatid cyst, protoscolices, silver nanoparticle, iron nanoparticle, copper nanoparticle, silica nanoparticle, in vitro
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