<i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice
Pelvic irradiation-induced mucositis secondarily leads to dysbiosis, which seriously affects patients’ quality of life after treatment. No safe and effective radioprotector or mitigator has yet been approved for clinical therapy. Here, we investigated the potential protective effects of fresh biomas...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/3/572 |
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| author | Charlotte Segers Mohamed Mysara Amelie Coolkens Shari Wouters Sarah Baatout Natalie Leys Sarah Lebeer Mieke Verslegers Felice Mastroleo |
| author_facet | Charlotte Segers Mohamed Mysara Amelie Coolkens Shari Wouters Sarah Baatout Natalie Leys Sarah Lebeer Mieke Verslegers Felice Mastroleo |
| author_sort | Charlotte Segers |
| collection | DOAJ |
| description | Pelvic irradiation-induced mucositis secondarily leads to dysbiosis, which seriously affects patients’ quality of life after treatment. No safe and effective radioprotector or mitigator has yet been approved for clinical therapy. Here, we investigated the potential protective effects of fresh biomass of <i>Limnospira indica</i> PCC 8005 against ionizing irradiation-induced mucositis and dysbiosis in respect to benchmark probiotic <i>Lacticaseibacillus rhamnosus</i> GG ATCC 53103. For this, mice were supplemented daily before and after 12 Gy X-irradiation of the pelvis. Upon sacrifice, food supplements’ efficacy was assessed for intestinal barrier protection, immunomodulation and changes in the microbiota composition. While both could not confer barrier protection or significant immunomodulatory effects, 16S microbial profiling revealed that <i>L. indica</i> PCC 8005 and <i>L. rhamnosus</i> GG could prevent pelvic irradiation-induced dysbiosis. Altogether, our data show that—besides benchmarked <i>L. rhamnosus</i> GG—<i>L. indica</i> PCC 8005 is an interesting candidate to further explore as a radiomitigator counteracting pelvic irradiation-induced dysbiosis in the presented in vivo irradiation–gut–microbiota platform. |
| first_indexed | 2024-03-11T07:00:28Z |
| format | Article |
| id | doaj.art-59f262d008d34aa6bcf9d735b0aee3c4 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T07:00:28Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-59f262d008d34aa6bcf9d735b0aee3c42023-11-17T09:16:41ZengMDPI AGAntioxidants2076-39212023-02-0112357210.3390/antiox12030572<i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in MiceCharlotte Segers0Mohamed Mysara1Amelie Coolkens2Shari Wouters3Sarah Baatout4Natalie Leys5Sarah Lebeer6Mieke Verslegers7Felice Mastroleo8Interdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumDepartment of Bioscience Engineering, University of Antwerp, 2020 Antwerp, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumInterdisciplinary Biosciences Group, Belgian Nuclear Research Centre, SCK CEN, 2400 Mol, BelgiumPelvic irradiation-induced mucositis secondarily leads to dysbiosis, which seriously affects patients’ quality of life after treatment. No safe and effective radioprotector or mitigator has yet been approved for clinical therapy. Here, we investigated the potential protective effects of fresh biomass of <i>Limnospira indica</i> PCC 8005 against ionizing irradiation-induced mucositis and dysbiosis in respect to benchmark probiotic <i>Lacticaseibacillus rhamnosus</i> GG ATCC 53103. For this, mice were supplemented daily before and after 12 Gy X-irradiation of the pelvis. Upon sacrifice, food supplements’ efficacy was assessed for intestinal barrier protection, immunomodulation and changes in the microbiota composition. While both could not confer barrier protection or significant immunomodulatory effects, 16S microbial profiling revealed that <i>L. indica</i> PCC 8005 and <i>L. rhamnosus</i> GG could prevent pelvic irradiation-induced dysbiosis. Altogether, our data show that—besides benchmarked <i>L. rhamnosus</i> GG—<i>L. indica</i> PCC 8005 is an interesting candidate to further explore as a radiomitigator counteracting pelvic irradiation-induced dysbiosis in the presented in vivo irradiation–gut–microbiota platform.https://www.mdpi.com/2076-3921/12/3/572ionizing radiationintestinemucositismicrobiomemicrobiotadysbiosis |
| spellingShingle | Charlotte Segers Mohamed Mysara Amelie Coolkens Shari Wouters Sarah Baatout Natalie Leys Sarah Lebeer Mieke Verslegers Felice Mastroleo <i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice Antioxidants ionizing radiation intestine mucositis microbiome microbiota dysbiosis |
| title | <i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice |
| title_full | <i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice |
| title_fullStr | <i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice |
| title_full_unstemmed | <i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice |
| title_short | <i>Limnospira indica</i> PCC 8005 Supplementation Prevents Pelvic Irradiation-Induced Dysbiosis but Not Acute Inflammation in Mice |
| title_sort | i limnospira indica i pcc 8005 supplementation prevents pelvic irradiation induced dysbiosis but not acute inflammation in mice |
| topic | ionizing radiation intestine mucositis microbiome microbiota dysbiosis |
| url | https://www.mdpi.com/2076-3921/12/3/572 |
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