Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer
Background and objective Epidermal growth factor receptor (EGFR) gene mutation in lung cancer is a reliable predictor of the efficacy of tyrosine kinase inhibitors, so detection of gene mutation has very important clinical significance. The aim of this study is to optimize conditions for restriction...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2011-08-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2011.08.01 |
| _version_ | 1818989226008510464 |
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| author | Fan YANG Kezhong CHEN Guanchao JIANG Jianfeng LI Jun WANG |
| author_facet | Fan YANG Kezhong CHEN Guanchao JIANG Jianfeng LI Jun WANG |
| author_sort | Fan YANG |
| collection | DOAJ |
| description | Background and objective Epidermal growth factor receptor (EGFR) gene mutation in lung cancer is a reliable predictor of the efficacy of tyrosine kinase inhibitors, so detection of gene mutation has very important clinical significance. The aim of this study is to optimize conditions for restriction enzyme-based mutant enriched detection of EGFR mutation in lung cancer specimens. Methods EGFR somatic mutations identified in DNA specimens from 251 lung adenocarcinomas with modified mutant enriched method and direct sequencing were compared. The sensitivity of the modified method was determined by serial dilution of mutant-carrying lung cancer cells in wild-type cells. Results 46 deletion mutations in exon 19 and 26 point mutations in exon 21 were found by sequencing, while modified method identified another 78 deletions in exon 19 and 57 substitutions in exon 21 with a mutation rate of 53.8%. Sensitivity of this modified method was 0.5% (mutant/wild type) in serial diluted cells. Conclusion The modified restriction enzyme-based method is convenient for clinical EGFR mutation screening in non-small cell lung cancer for its simpleness, cost-saving and high sensitivity. |
| first_indexed | 2024-12-20T19:35:06Z |
| format | Article |
| id | doaj.art-59f29bb5f9504883aa8283134724c77c |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-20T19:35:06Z |
| publishDate | 2011-08-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-59f29bb5f9504883aa8283134724c77c2022-12-21T19:28:41ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872011-08-0114863764110.3779/j.issn.1009-3419.2011.08.01Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung CancerFan YANGKezhong CHENGuanchao JIANGJianfeng LIJun WANGBackground and objective Epidermal growth factor receptor (EGFR) gene mutation in lung cancer is a reliable predictor of the efficacy of tyrosine kinase inhibitors, so detection of gene mutation has very important clinical significance. The aim of this study is to optimize conditions for restriction enzyme-based mutant enriched detection of EGFR mutation in lung cancer specimens. Methods EGFR somatic mutations identified in DNA specimens from 251 lung adenocarcinomas with modified mutant enriched method and direct sequencing were compared. The sensitivity of the modified method was determined by serial dilution of mutant-carrying lung cancer cells in wild-type cells. Results 46 deletion mutations in exon 19 and 26 point mutations in exon 21 were found by sequencing, while modified method identified another 78 deletions in exon 19 and 57 substitutions in exon 21 with a mutation rate of 53.8%. Sensitivity of this modified method was 0.5% (mutant/wild type) in serial diluted cells. Conclusion The modified restriction enzyme-based method is convenient for clinical EGFR mutation screening in non-small cell lung cancer for its simpleness, cost-saving and high sensitivity.http://dx.doi.org/10.3779/j.issn.1009-3419.2011.08.01Lung neoplasmsEpidermal growth factor receptorMutation |
| spellingShingle | Fan YANG Kezhong CHEN Guanchao JIANG Jianfeng LI Jun WANG Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer Chinese Journal of Lung Cancer Lung neoplasms Epidermal growth factor receptor Mutation |
| title | Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer |
| title_full | Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer |
| title_fullStr | Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer |
| title_full_unstemmed | Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer |
| title_short | Modified Restriction Enzyme-based Detection of EGFR Mutations in Lung Cancer |
| title_sort | modified restriction enzyme based detection of egfr mutations in lung cancer |
| topic | Lung neoplasms Epidermal growth factor receptor Mutation |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2011.08.01 |
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